Identification of a Bioactive Compound, Violacein, from Microbulbifer sp. Isolated from a Marine Sponge Hymeniacidon sinapium on the West Coast of Korea
1
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of 25℃, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.
Abstract
Microbial secondary metabolites of marine organisms are regarded as major sources of structurally and biologically novel compounds with numerous potential uses. Sponge-microbe associations are among the most interesting sources for exploring bioactive compounds. In this study, the bacterial strain Microbulbifer sp. (127CP7-12) was isolated from the Asian marine sponge Hymeniacidon sinapium collected at an intertidal zone on the west coast of Korea. Cultured bacteria produced a violet pigment, and optimal culture conditions for violet pigment production were investigated. Maximum production of the violet pigment from the strain culture was observed under the conditions of 25℃, pH 6.0, and 3% NaCl. Acetone provided better extraction of the pigment from fermented broth compared with ethanol and methanol. The proposed structure of the major component in the extracted crude pigment was determined via high-performance liquid chromatography, nuclear magnetic resonance, mass spectrometry, and UV spectra analyses, which showed that the metabolite was the promising bioactive compound violacein. This study describes the examination of marine bioactive materials from microbe-engaged metabolites and the ecological implications of the sponge-microbe association in a changing ocean.
참고문헌 (30)
- Halpern BS, "A global map of human impact on marine ecosystems", Science, 319, pp.948~952 (2008)
- Belkin IM, "Rapid warming of large marine ecosystems", Progress in Oceanography, 81, pp.207~213 (2009)
- Poloczanska ES, "Global imprint of climate change on marine life", Nature Climate Change, 3, pp.919~925 (2013)
- Morán XAG, "Presented at the Proc. R. Soc. B", ~ (2015)
- Barton AD, "Anthropogenic climate change drives shift and shuffle in North Atlantic phytoplankton communities", Proceedings of the National Academy of Sciences, 113, pp.2964~2969 (2016)
- Dias DA, "A historical overview of natural products in drug discovery", Metabolites, 2, pp.303~336 (2012)
- Harvey AL, "The re-emergence of natural products for drug discovery in the genomics era", Nature Reviews. Drug Discovery, 14, pp.111~129 (2015)
- D'Orazio N, "Marine bioactives: pharmacological properties and potential applications against inflammatory diseases", Marine Drugs, 10, pp.812~833 (2012)
- Skropeta D, "Recent advances in deep-sea natural products", Natural Product Reports, 31, pp.999~1025 (2014)
- Choi SY, "High-level production of violacein by the newly isolated Duganella violaceinigra str. NI28 and its impact on Staphylococcus aureus", Scientific Reports, 5, pp.15598~ (2015)
- Laport MS, "Marine sponges: potential sources of new antimicrobial drugs", Curr. Pharm. Biotechnol, 10, pp.86~105 (2009)
- El-Shitany NA, "Evaluation of the anti-inflammatory, antioxidant and immunomodulatory effects of the organic extract of the red sea marine sponge xestospongia testudinaria against carrageenan induced rat paw inflammation", PLoS One, 10, pp.e0138917~ (2015)
- Li Z, "Advances in marine microbial symbionts in the china sea and related pharmaceutical metabolites", Marine Drugs, 7, pp.113~129 (2009)
- Matz C, "Off the hook--how bacteria survive protozoan grazing", Trends Microbiol, 13, pp.302~307 (2005)
- Matz C, "Marine biofilm bacteria evade eukaryotic predation by targeted chemical defense", PLoS One, 3, pp.e2744~ (2008)
- Rusch DB, "The Sorcerer II Global Ocean Sampling expedition: northwest Atlantic through eastern tropical Pacific", PLoS Biol, 5, pp.e77~ (2007)
- Seshadri R, "CAMERA:a community resource for metagenomics", PLoS Biology, 5, pp.e75~ (2007)
- 박미현, "Identification of Genes Suitable for DNA Barcoding of Morphologically Indistinguishable Korean Halichondriidae Sponges", Molecules and Cells, 23(2), pp.220~227 (2007), 한국분자세포생물학회
- Hoshino S, "Contrasting genetic structure of two Pacific Hymeniacidon species", Hydrobiologia, 603, pp.313~326 (2008)
- Fuller T, "Molecular investigation of the invasive sponge Hymeniacidon sinapium (de Laubenfels, 1930) in Elkhorn Slough, California", Aquatic Invasions, 8, pp.59~66 (2013)
- 정종빈, "주황해변해면(Hymeniacidon sinapium) 공생세균 군집의 계절적 차이", 미생물학회지, 48(4), pp.262~269 (2012), 한국미생물학회[DOI]
- Yang LH, "Effect of agitation on violacein production in Pseudoalteromonas luteoviolacea isolated from a marine sponge", Lett. Appl. Microbiol, 44, pp.625~630 (2007)
- Ventosa A, "Salinicoccus roseus gen. nov., sp. nov., a New Moderately Halophilic Gram-Positive Coccus", Syst. Appl. Microbiol, 13, pp.29~33 (1990)
- Wakabayashi M, "Isolation and characterization of Microbulbifer species 6532A degrading seaweed thalli to single cell detritus particles", Biodegradation, 23, pp.93~105 (2012)
- Rettori D, "Production, extraction and purificationof violacein: an antibiotic pigment producedby Chromobacterium violaceum", World J. Microbiol. Biotechnol, 14, pp.685~688 (1998)
- Nakamura Y, "Isolation of a psychrotrophic bacterium from the organic residue of a water tank keeping rainbow trout and antibacterial effect of violet pigment produced from the strain", Biochem. Eng. J, 12, pp.79~86 (2002)
- Yoon JH, "Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov", Int. J. Syst. Evol. Microbiol, 53, pp.1357~1361 (2003)
- Webster NS, "Temperature thresholds for bacterial symbiosis with a sponge", The ISME J, 2, pp.830~842 (2008)
- Goodwin C, "Effects of ocean acidification on sponge communities", Mar. Ecol, 35, pp.41~49 (2014)
- Das S, "Ocean acidification and marine microorganisms:responses and consequences", Oceanologia, 57, pp.349~361 (2015)