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Zooshikella sp. JE-34가 생산하는 Prodigiosin계 색소의 분리 및 최적 배양조건

Isolation and Optimal Culture Conditions of Prodigiosin-like Pigment Produced by Zooshikella sp. JE-34

이경준|김만철|허문수|김주상
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2009.09|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제37권 제3호|pp. 219-255

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참고문헌

키워드

Red pigmentoptimal conditionsZooshikella sp. JE-34prodigiosin-like compound
주제분류생물학

초록

A bacterial strain, JE-34, producing a high concentration of red pigment was isolated from a sediment in East China Sea. It was identified as Zooshikella sp. JE-34 based on the 16S rRNA gene sequence analysis. The red pigment was purified by solvent extraction and HPLC was identified as prodigiosin-like compound. Nutritional and cultural conditions were optimized for the production of prodigiosin-like pigment in the flask level. Optimal culture conditions were at initial medium pH 6.0~7.0, 30℃ and 4 days incubation. For carbon and, nitrogen sources were soluble starch and malt extract.

Abstract

A bacterial strain, JE-34, producing a high concentration of red pigment was isolated from a sediment in East China Sea. It was identified as Zooshikella sp. JE-34 based on the 16S rRNA gene sequence analysis. The red pigment was purified by solvent extraction and HPLC was identified as prodigiosin-like compound. Nutritional and cultural conditions were optimized for the production of prodigiosin-like pigment in the flask level. Optimal culture conditions were at initial medium pH 6.0~7.0, 30℃ and 4 days incubation. For carbon and, nitrogen sources were soluble starch and malt extract.

참고문헌 (25)

  1. Allen, E. G, "Conditions of the colour change of prodigiosin", Nature, 216, pp.929~931 (1967)
  2. Choi, B. B, "Studies on the effect of glyoxylate on the biosynthesis of prodigiosin in Serratia marcescens", Kor. J. Food Nutr., 10, pp.475~479 (1997)
  3. Clements-Jewery, S, "The reversal of glucose repressed prodigiosin production in serratia marcscens by the cyclic 3', 5'-adenosine monophosphate inhibitor theophyline", Biochem. Biophys. Acta., 15, pp.421~422 (1976)
  4. Custro, A. J, "Products from Serratia marcescens", J. Org. Chem., 24, pp.455~459 (1959)
  5. D’Alessio, R, "Synthesis and immunosuppressive activity of novel prodigiosin derivatives", J. Med. Chem, 43, pp.2557~2565 (2000)
  6. Deol, B. S, "Isolation and structure confirmation of norprodigiosin from a Serratia marcescens mutant", Aus. J. Chem, 27, pp.2657~2662 (1974)
  7. Huh, J. E, "Prodigiosin isolated from Hahella chejuensis suppresses lipopolysaccharide-induced NO production by inhibiting p38 MARK, JNK and NK-κB activation in murine peritoneal macrophages", Int. Immunopharmacol, 7, pp.1825~1833 (2007)
  8. Hwang, E. I, "Screening system for chitin systhase II inhibitors from natural resources and its inhibitor prodigiosin", J. Microbiol. Biotechnol, 10, pp.251~257 (2000)
  9. Jeong, H. Y, "Genomic blueprint of Hahella chejuensis, a marine microbe producing an algicidal agent", Nucl. Acids Res., 33, pp.7066~7073 (2005)
  10. Kalesperis, G. S, "Toxigenic studies with the antibiotic pigment from Serratia marcescans", Can. J. Microbiol, 21, pp.213~220 (1975)
  11. Kim, C. H, "Isolation and characteristics of prodigiosin-like red pigment produced by Serratia sp. KH-95", Kor. J. Appl. Microbiol. Biotechnol., 26, pp.283~289 (1998)
  12. Kim, D, "Biosynthesis of antibiotic prodiginines in the marine bacterium Hahella chejuensis KCTC 2396", J. Appl. Microbiol., 102, pp.937~944
  13. Lim, D. V, "Biosynthesis of prodigiosin by non-proliferating wildtype Serratia marcescens and mutant deficient in catabolism of alanine, histidine and proline", J. Bacteriol, 129, pp.124~129 (1977)
  14. Montaner, B, "Prodigiosin from the supernatant of Serratia marcescens induces apoptosis in haematopoietic cancer cell lines", Br J. Pharmacol, 131, pp.585~593 (2000)
  15. Mortellaro, A, "New immunosuppressive drug PNU156804 blocks IL-2-dependent proliferation and NF-kappa B and AP-1 activation", J. Immunol, 162, pp.7102~7109 (1999)
  16. Natarajan, V, "Biosynthesis UV stable pigment prodigiosin", Paintindia, 45, pp.23~33 (1995)
  17. Qadri, S. M. H, "Role of methionine in biosynthesis of prodigiosin by Serratia marcescens", J. Bacteriol., 116, pp.1191~1198 (1973)
  18. Qadri, S. M. H, "Incorporation of amino acid carbon into prodigiosin synthesized by nonproliferating cells of Serratia marcescens", Can. J. Microbiol, 20, pp.461~468 (1974)
  19. Slater, H, "Phosphate availability regulates biosynthesis of two antibiotics, prodigiosin and carbapenem, in Serratia via both quorum-sensing-dependent and -independent pathways", Mol. Microbiol., 47, pp.303~320 (2003)
  20. Sole, M, "The effect of pH on prodigiosin production by non-proliferating cells of Serratia marcescens", Lett. Appl. Microbiol., 19, pp.341~344 (1994)
  21. Song, M. J, "Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95", J. Biosci. Bioeng., 101, pp.157~161 (2006)
  22. Tanaka, S, "Fermentation processes in screening for new bioactive substances. in: The search for bioactive compound from microorganism.", pp.303~326 (1992), Springer-Verlag
  23. Wasserman, H. H, "Biosynthesis of prodigiosin Incorporation patterns of 13-carbon-labeled alanine, proline, glycine, and serine elucidated by Fourier transform nuclear magnetic resonance", J. Am. Chem. Sor, 95, pp.6874~6875 (1973)
  24. Witney, F. R, "Phosphate inhibition of secondary metabolism in Serratia marcescens", Appl. Environ. Microbiol, 33, pp.1042~1046 (1977)
  25. Yi, H, "Zooshikella ganghwensis gen. nov., sp. nov., isolated from tidal flat sediments", Int. J. Syst. Evol. Microbiol, 53, pp.1013~1018 (2003)

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