넙치(Paralichthys olivaceus)의 비특이적 면역반응 및 병 저항성에 대한 Bacillus subtilis MD-02의 효과
Dietary effect of Bacillus subtilis MD-02 on Innate Immune Response and Disease Resistance in Olive Flounder, Paralichthys olivaceus
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초록
Among several marine-derived microorganisms isolated from the coast of Jeju Island that had antimicrobial activity against fish disease pathogens, Bacillus subtilis MD-02 was tested for its dietary effect on the innate immune response and disease resistance of olive flounder. Strain MD-02 was fed to the olive flounder at a concentration of 1.2 × 104, 1.2 × 106, or 1.2 × 108 CFU/100 g, respectively. Consequently, the hematocrit was higher in these three groups than that in the control group at 4 weeks, and the aspartate aminotransferase and alanine aminotransferase levels were decreased in the 1.2 × 108 and 1.2 × 104 CFU/100 groups compared with the control group levels. The amylase activity and total protein were significantly increased in the 1.2 × 104 CFU/100 g group at 3 weeks. The innate immune response, determined from the lysozyme and macrophage activities, was higher in the 1.2 × 108 CFU/100 g group than in the control group. In addition, treatment of the olive flounders with Streptococcus parauberis at 1.2 × 106 CFU/ml confirmed the mortality rate, which was 100% in the control group and 40−60% in the groups fed B. subtilis MD-02, indicating that the fish had resistance to fish disease pathogens. Therefore, it was confirmed that when fed MD-02, olive flounder builds an innate immune response and acquires resistance to fish disease pathogens, indicating that B. subtilis MD-02 can be developed as a beneficial feed additive.
Abstract
Among several marine-derived microorganisms isolated from the coast of Jeju Island that had antimicrobial activity against fish disease pathogens, Bacillus subtilis MD-02 was tested for its dietary effect on the innate immune response and disease resistance of olive flounder. Strain MD-02 was fed to the olive flounder at a concentration of 1.2 × 104, 1.2 × 106, or 1.2 × 108 CFU/100 g, respectively. Consequently, the hematocrit was higher in these three groups than that in the control group at 4 weeks, and the aspartate aminotransferase and alanine aminotransferase levels were decreased in the 1.2 × 108 and 1.2 × 104 CFU/100 groups compared with the control group levels. The amylase activity and total protein were significantly increased in the 1.2 × 104 CFU/100 g group at 3 weeks. The innate immune response, determined from the lysozyme and macrophage activities, was higher in the 1.2 × 108 CFU/100 g group than in the control group. In addition, treatment of the olive flounders with Streptococcus parauberis at 1.2 × 106 CFU/ml confirmed the mortality rate, which was 100% in the control group and 40−60% in the groups fed B. subtilis MD-02, indicating that the fish had resistance to fish disease pathogens. Therefore, it was confirmed that when fed MD-02, olive flounder builds an innate immune response and acquires resistance to fish disease pathogens, indicating that B. subtilis MD-02 can be developed as a beneficial feed additive.
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