김치 유산균 배양물의 부상용 배합사료내 첨가시 넙치P(aralichthys olivaceus) 치어의 성장, 체조성 및 면역성 반응에 미치는 효과
Effects of the Inclusion of Kimchi Lactic Acid Bacterial Culture in Extruded Pellets on the Growth, Body Composition and Immune Response of Juvenile Olive Flounder Paralichthys olivaceus
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters,lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.
Abstract
This study was performed to determine effects of the supplementation of Kimchi lactic acid bacterial culture in extruded pellets (EP) on the growth, body composition, blood chemistry and immune response of olive flounder Paralichthys olivaceus. Four hundred eighty individuals averaging 16.1 g were randomly distributed into 12, 180L flow-through tanks (forty fish per tank). Four concentrations of Kimchi lactic acid bacterial culture (KL) were prepared: Control (0%), 0.1%, 0.2% and 0.5%. Three concentrations (0.1%, 0.2% and 0.5%) of Kimchi lactic acid bacterial culture were each diluted to 10% of EP weight and then fully absorbed by EP for 10 minutes. Each diet was fed to triplicate groups of fish. Fish were hand-fed to apparent satiation twice a day for 8 weeks. At the end of the 8-week feeding trial, the plasma lysozyme and bacterial activities of fish were determined. In addition, the cumulative mortality of fish was monitored for 8 days after their artificial infection with Edwardsiella tarda. The weight gain, specific growth rate, feed efficiency ratio, protein efficiency ratio, protein retention, hepatosomatic index and condition factor of fish were not affected by dietary supplementation with KL. None of the proximate composition, plasma parameters,lysozyme or bactericidal activities of fish was affected by dietary supplementation with KL. However, the cumulative mortalities of fish fed EP containing 0.1% and 0.5% Kimchi lactic acid bacterial culture were relatively low compared to that of fish fed the control diet. In conclusion, dietary supplementation with KL did not effectively improve growth, feed utilization, body composition, plasma chemistry, lysozyme, bactericidal activities or immune response of olive flounder after E. tarda infection under these experimental conditions.
참고문헌 (34)
- AOAC, "Official methods of analysis, 15th edition" (1990), Association of Official Analytical Chemists
- Cho SH, "Compensatory growth of juvenile flounder Paralichthys olivaceus L. and changes in biochemical composition and body condition indices during starvation and after refeeding in winter season", J World Aquac Soc, 36, pp.508~514 (2005)[DOI]
- Cho SH, "Effects of putative growth or health-enhancing dietary additives on juvenile olive flounder, Paralichthys ol-ivaceus, performance", J World Aquac Soc, 42, pp.90~95 (2011)[DOI]
- Cho S, "Onion powder in the diet of the olive flounder, Paralichthys olivaceus: Effects on the growth, body composition, and lysozyme activity", J World Aquac Soc, 43, pp.30~38 (2012)[DOI]
- Cho SH, "Effect of feeding ratio on growth and body composition of juvenile olive flounder Paralichthys olivaceus fed extruded pellets during the summer season", Aquaculture, 251, pp.78~84 (2006)[DOI]
- Cho SH, "Compensatory growth of juvenile olive flounder Paralichthys olivaceus L. and changes in proximate composition and body condition indexes during fasting and after refeeding in summer season", J World Aqua Soc, 37, pp.168~174 (2006)[DOI]
- Cho SH, "Effect of daily feeding ratio on growth and body composition of sub-adult olive flounder, Paralichthys olivaceus, fed an extruded diet during the summer season", J World Aquac Soc, 38, pp.68~73 (2007)
- Cho SH, "Effect of dietary inclusion of various sources of green tea on growth, body composition and blood chemistry of juvenile olive flounder Paralichthys olivaceus", Fish Physiol Biochem, 33, pp.49~57 (2007)[DOI]
- S.H. Cho, "Effects of Dietary Scutellaria baicalensis Extract on Growth, Feed Utilization and Challenge Test of Olive Flounder (Paralichthys olivaceus)", ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 26(1), pp.90~96 (2013), 아세아·태평양축산학회[DOI]
- 최세민, "치어기 넙치에 있어서 사료 첨가제로서 송강약돌의 이용 가능성", 한국양식학회지, 17(1), pp.39~45 (2004), 한국양식학회
- Duncan DB, "Multiple range and multiple F tests", Biometrics, 11, pp.1~42 (1955)
- Han JH, "Effect of cultural materials from Kimchi lactic acid bacteria on growth performance and prevention of disease in pigs", pp.2006
- Kim KD, "Requirement of dietary n-3 highly unsaturated fatty acids for Japanese flounder (Paralichthysolivaceus)", J Aquaculture, 229, pp.315~323 (2004)[DOI]
- Kim DS, "Utilization of obosan (dietary herbs)Ⅰ. Effects on survival, growth, feed conversion ratio and condition factor in olive flounder, Paralichthys olivaceus", J Aquaculture, 11, pp.213~221 (1998)
- Kim JH, "Utilization of dietary herb obosan Ⅲ. Growth of juvenile olive flounder, Paralichthys olivaceus", J Aquaculture, 13, pp.231~238 (2000)
- 김정대, "일일 및 격일 습사료 공급방법이 육성 넙치의 성장과 영양소 이용효율에 미치는 영향", 한국양식학회지, 15(1), pp.15~21 (2002), 한국양식학회
- Kim K, "Effects of dietary Chlorella ellipsoidea supplementation on growth, blood characteristic and whole-body composition in juvenile olive flounder Paralichthys olivaceus", J World Aquac Soc, 33, pp.425~431 (2002)
- 김종태, "키토산 사료첨가제 급이가 양식넙치의 생장과 품질특성에 미치는 영향", Journal of Chitin and Chitosan, 10(3), pp.121~127 (2005), 한국키틴키토산학회
- 김경덕, "사료급이율이 여름철에 사육된 넙치 성어의 성장 및 체조성에 미치는 영향", 한국양식학회지, 22(1), pp.1~4 (2009), 한국양식학회
- 김강웅, "넙치 사료내 어분대체 단백질원으로써 오징어간분말의 평가", 수산해양교육연구, 23(3), pp.461~467 (2011), 한국수산해양교육학회
- Kim KT, "Effects of dietary inclusion of various concentrations of Scutellaria baicalensis Georgi extract on growth, body composition, serum chemistry and challenge test of far eastern catfish (Silurus asotus)", Aquacult Res (2013)[DOI]
- KOSIS, "Korean Statistical Information Service", ~ (2013)
- Kikuchi K, "Use of defatted soybean meal as a substitute for fish meal in diets of Japanese flounder (Paralichthysolivaceus)", Aquaculture, 179, pp.3~11 (1999)[DOI]
- Kikuchi K, "Partial replacement of fish meal with corn gluten meal in diets for Japanese flounder Paralichthys olivaceus", J World Aquac Soc, 30, pp.357~363 (1999)[DOI]
- Kikuchi K, "Utilization of feather meal as a protein source in the diet of juvenile flounder", Fish Sci, 60, pp.203~206 (1994)
- Kikuchi K, "Use of meat and bone meal as protein source in the diet of juvenile Japanese flounder", Fish Sci, 63, pp.29~32 (1997)
- 권문경, "숯첨가 사료투여가 넙치의 면역반응에 미치는 영향", 한국어병학회지, 15(1), pp.17~24 (2002), 한국어병학회
- Lee KH, "Utilization of obosan (dietary herbs) Ⅱ. Muscle quality of olive flounder, Paralichthys olivaceus fed with diet containing obosan", J Aquaculture, 11, pp.319~325 (1998)
- Lee SM, "Dietary protein requirement of young Japanese flounder Paralichthys olivaceus fed isocaloric diets", Fish Sci, 68, pp.158~164 (2002)[DOI]
- Miles AA, "The estimate of the bactericidal power of the blood", J Hyg, 38, pp.873~885 (1938)
- 박상언, "배합사료에 미역, 어보산 및 고추냉이 첨가가 넙치 치어의 성장, 체성분, 혈액성상 및 비특이적 면역능에 미치는 효과", 한국양식학회지, 16(4), pp.210~215 (2003), 한국양식학회
- Parry RM, "A rapid and sensitive assay of muramidase", Proc Soc Exp Biol Med, 119, pp.384~386 (1965)
- 원경미, "β-1,3", 1,6-linked glucan 첨가사료가 넙치의 비특이적면역반응에 미치는 영향, 2004, pp.17(1), 29~38[DOI]
- Yoo B, "Bacterial action by complement of fish serum", J Fish Pathol, 5, pp.9~18 (1992)