MITF 발현 조절을 통한 개똥쑥 추출물 및 아테미시닌의 미백 개선 효과
The Skin Whitening Effect of Artemisia annua L. Extracts and Artemisinin through Modulation of MITF Expression
2
조회
0
다운로드
6
인용
0
참고문헌
키워드
초록
Artemisinin is known as a constituent of the medicinal plant, Artemisia annua L. and has been used in folk medicine to treat various ailments for centuries. In studies to investigate the whitening effect of Artemisia annua L. extracts and artemisinin as an active ingredient for whitening cosmetics, the effects of Artemisia annua L. extracts and artemisinin on melanogenesis in B16-F10 melanoma cells were identified. In a cell viability assay, Artemisia annua L. extracts and artemisinin did not show any obvious cytotoxicity, and they also significantly reduced both tyrosinase activity and the cellular melanin content in a concentration-dependent manner. Furthermore, it was found that artemisinin decreased α-MSH (melanocyte-stimulating hormone)-induced tyrosinase activity and microphthalmia associated transcription factor (MITF) protein expression. These data indicate that Artemisia annua L. extracts and artemisinin attenuates α-MSH-stimulated melanin synthesis by modulating MITF expression and that they might be a useful therapeutic agent for treating hyperpigmentation and an ingredient of whitening cosmetics.
Abstract
Artemisinin is known as a constituent of the medicinal plant, Artemisia annua L. and has been used in folk medicine to treat various ailments for centuries. In studies to investigate the whitening effect of Artemisia annua L. extracts and artemisinin as an active ingredient for whitening cosmetics, the effects of Artemisia annua L. extracts and artemisinin on melanogenesis in B16-F10 melanoma cells were identified. In a cell viability assay, Artemisia annua L. extracts and artemisinin did not show any obvious cytotoxicity, and they also significantly reduced both tyrosinase activity and the cellular melanin content in a concentration-dependent manner. Furthermore, it was found that artemisinin decreased α-MSH (melanocyte-stimulating hormone)-induced tyrosinase activity and microphthalmia associated transcription factor (MITF) protein expression. These data indicate that Artemisia annua L. extracts and artemisinin attenuates α-MSH-stimulated melanin synthesis by modulating MITF expression and that they might be a useful therapeutic agent for treating hyperpigmentation and an ingredient of whitening cosmetics.
참고문헌 (24)
- Lim J. Y., "Melanocyte biology and skin pigmentation", Nature, 445, pp.843~850 (2007)
- 윤미연, "질경이 seed 추출물의 항산화 활성 및 미백작용에 관한 연구", 한국인체미용예술학회지, 14(2), pp.259~269 (2013), 한국인체미용예술학회
- Prota G., "Some new aspects of eumelanin chemistry", Progress in Clinical and Biological Research, 256, pp.101~124 (1998)
- Hearing V. J., "Mammalian tyrosinase-the critical regulatory control point in melanocyte pigmentation", International Journal of Biochemistry, 19, pp.1141~1147 (1987)
- Jackson,I. J, "A second tyrosinase-related protein, TRP-2, maps to and is mutated at the mouse slaty locus", The EMBO Journal, 11(2), pp.527~535 (1992)
- Kobayashi T., "Tyrosinase related protein 1(TRP1)functions as a DHICA oxidase in melanin biosynthesis", The EMBO Journal, 13(24), pp.5818~5825 (1994)
- Busca R., "Cyclic Camp a key messenger in the regulation of skin pigmentation", Pigment Cell Research, 13, pp.60~69 (2000)
- Wakamatsu K., "Characterisation of ACTH peptides in human skin and their activation of the melanocortin-1 receptor", Pigment Cell Research, 10, pp.288~297 (1997)
- Costin G. E., "Human skin pigmentation : melanocytes modulate skin color in response to stress", The FASEB Journal, 21, pp.976~994 (2007)
- Hodgkinson C. A., "Mutations at the mouse microphthalmia locus are associated with defects in a gene encoding a novel basic-helix-loop-helix-zipper protein", Cell, 74, pp.395~404 (1993)
- Yasumoto K., "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosianse family genes", The Journal of Biological Chemistry, 272, pp.503~509 (1997)
- Briganti S., "Chemical and instrumental approaches to treat hyperpigmentation", Pigment Cell Research, 16, pp.101~110 (2003)
- Maeda K., "Arbutin : mechanism of its depigmenting action in human melanocyte culture", Journal of Pharmacology and Experimental Therapeutics, 276, pp.765~769 (1996)
- Hien T. T., "Qinghaosu", Lancet, 341, pp.603~608 (1993)
- Bhakuni R. S., "Secondary metabolites of Artemisa annua and their biological activity", Current Science India, 80, pp.35~48 (2001)
- Efferth T., "Willmar Schwabe Award 2006 : antiplasmodial and antitumor activity of artemisinin-from bench to bedside", Planta Medica, 73, pp.299~309 (2007)
- Ferreira J. F., "Flavonoids from Artemisa annua L. as antioxidants and their potential synergism with artemisinin aganinst malaria and cancer", Molecules, 15, pp.3135~3170 (2010)
- 김미경, "블루베리 잎 추출물의 항산화 및 항염증 효과", 한국인체미용예술학회지, 15(4), pp.109~120 (2014), 한국인체미용예술학회
- 김연주, "조협과 천궁혼합물의 미백효과", 한국인체미용예술학회지, 8(2), pp.3~14 (2007)
- Yasumoto K., "Functional analysis of microphthalmia-associated transcription factor in pigment cell-specific transcription of the human tyrosinase family gene", The Journal of Biological Chemistry, 272, pp.503~509 (1997)
- Steingrimsson E., "Melanocytes and the microphthalmia transcription factor network", Annual Review of Genetics, 38, pp.365~411 (2004)
- Yamaguchi Y., "Physiological factors that regulate skin pigmentation", Biofactors, 35(2), pp.193~199 (2009)
- Goding C. R., "Melanocytes : the new Black", The International Journal of Biochemistry & Cell Biology, 39, pp.275~279 (2007)
- Bentley N. J., "Melanocyte-specific expression of the human tyrosinase promoter : activation by the microphthalmia gene product and role of the initiator", Molecular and Cellular Biology, 14, pp.7996~8006 (1994)