Production of α-Glucosidase Inhibitor and 1-Deoxynojirimycin by Bacillus subtilis MORI
2
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Galactose and soybean meal were selected as the best carbon and nitrogen sources, repectively, for the efficient production of α-glucosidase inhibitor (AGI) and 1-deoxynojirimycin (DNJ) by a newly isolated Bacillus subtilis MORI. The optimal concentrations of the galactose and soybean meal for the production of AGI and DNJ were investigated by response surface methodology. For the production of AGI, the optimal galactose and soybean meal concentrations were 4.3% (w/v) and 3.2% (w/v), respectively, and for DNJ, 4.5% (w/v) and 3.0% (w/v), respectively. The nearly identical optimal concentrations of galactose and soybean meal for the production of both AGI and DNJ indicated a close correlation between the production of AGI and DNJ. The maximum production of AGI (50,880 GIU/ml) and DNJ (824 μg/ml) obtained from the statistically optimized medium in a jar fermenter was 2.33 and 2.38-fold, respectively, higher than those (21,798 GIU/ml and 346 μg/ml, respectively) of the pre-optimized medium. The production of both AGI and DNJ was greatly increased by jar fermentation (AGI of 38,524 GIU/ml and DNJ of 491 μg/ml) compared with flask fermentation.
Abstract
Galactose and soybean meal were selected as the best carbon and nitrogen sources, repectively, for the efficient production of α-glucosidase inhibitor (AGI) and 1-deoxynojirimycin (DNJ) by a newly isolated Bacillus subtilis MORI. The optimal concentrations of the galactose and soybean meal for the production of AGI and DNJ were investigated by response surface methodology. For the production of AGI, the optimal galactose and soybean meal concentrations were 4.3% (w/v) and 3.2% (w/v), respectively, and for DNJ, 4.5% (w/v) and 3.0% (w/v), respectively. The nearly identical optimal concentrations of galactose and soybean meal for the production of both AGI and DNJ indicated a close correlation between the production of AGI and DNJ. The maximum production of AGI (50,880 GIU/ml) and DNJ (824 μg/ml) obtained from the statistically optimized medium in a jar fermenter was 2.33 and 2.38-fold, respectively, higher than those (21,798 GIU/ml and 346 μg/ml, respectively) of the pre-optimized medium. The production of both AGI and DNJ was greatly increased by jar fermentation (AGI of 38,524 GIU/ml and DNJ of 491 μg/ml) compared with flask fermentation.
참고문헌 (42)
- DeFronzo RA, "Pathogenesis of type 2 diabetes mellitus", Med. Clin. North Am, 88, pp.787~835 (2004)
- Frandsen TP, "Plant α-glucosidases of glycoside hydrolase family 31. Molecular properties, substrate specificity, reaction mechanism, and comparison with family members of different origin", Plant Mol. Biol, 37, pp.1~13 (1998)
- Alain DB, "Postprandial hyperglycaemia and α-glucosidase inhibitors", Diabetes Res. Clin. Pract, 40, pp.51~55 (1998)
- Floris AV, "α-Glucosidase inhibitors for patients with type 2 diabetes", Diabetic Care, 28, pp.154~162 (2005)
- Holman RR, "Assessing the potential for α-glucosidase inhibitors in prediabetic states", Diabetes Res. Clin. Pract, 40, pp.21~25 (1998)
- Patricia MH, "Effects of a medical food containing an herbal α-glucosidase on postprandial glycaemia and insulinemia in healthy adults", J. Am. Diet Assoc, 105, pp.65~71 (2005)
- Clissold SP, "Acarbose, a preliminary review of its pharmacodynamic and pharmacokinetic properties, and therapeutic potential", Drugs, 35, pp.214~243 (1998)
- Toeller M, "α-Glucosidase inhibitors in diabetes: efficacy in NIDDM subjects", Eur. J. Clin. Invest, 24, pp.31~35 (1994)
- Murai A, "Control of postprandial hyperglycaemia by galactosyl maltobionolactone and its novel anti-amylase effect in mice", Life Sci, 71, pp.1405~1415 (2002)
- Chen J, "Anti-α-glucosidase activity of Chinese traditionally fermented soybean (douche)", Food Chem, 103, pp.1091~1096 (2007)
- Kwon DY, "Antidiabetic effects of fermented soybean products on type 2 diabetes", Nutr. Res, 30, pp.1~13 (2010)
- Yoshikawa M, "Absolute stereostructure of potent α-glucosidase inhibitor, salacinol, with unique thiosugar sulfonium sulfate inner salt structure from Salacia reticulata", Bioorg. Med. Chem, 10, pp.1547~1554 (2002)
- Liu Y, "Synthesis of xanthone derivatives with extended π-sytems as α-glucosidase inhibitors:Insight into the probable binding mode", Bioorg. Med. Chem, 15, pp.2810~2814 (2007)
- Muraoka O, "Synthesis and biological evaluation of deoxy salacinols, the role of polar substituents in the side chain on the α-glucosidase inhibitory activity", Bioorg. Med. Chem, 14, pp.500~509 (2006)
- Iwasa R, "Studies on validamycins, new antibiotic I. Streptomyces hygroscopicus var. limoneus. validamycin producing organiam", J. Antibiotechnol, 23, pp.595~602 (1970)
- Schmidt DD, "α-glucosidase inhibitors, new complex oligosaccharides of microbial origin", Naturwissenschaften, 64, pp.535~536 (1977)
- Kameda Y, "New cyclitols, degradation of validamycin A by Flavobacterium saccharophilum", J. Antibiot.(Tokyo), 33, pp.1573~1574 (1980)
- Zhu YP, "Improvement of the antioxidant activity of Chinese traditional fermented okara (Meitauza)using Bacillus subtilis B2", Food Control, 19, pp.654~661 (2008)
- Zhu YP, "Effects of sources of carbon and nitrogen on production of α-glucosidase inhibitor by a newly isolated of Bacillus subtilis B2", Food Chem, 109, pp.737~742 (2008)
- Romaniouk AV, "Synthesis of a novel photoaffinity derivative of 1-deoxynojirimycin for active sitedirected labeling of glucosidase I", Glycobiol, 14, pp.301~310 (2004)
- Yagi M, "The structure of moraoline, a piperidine alkaloid from Morus species", Nippon Nougei kagaku kaishi, 50, pp.571~572 (1976)
- Kimura T, "Simple and rapid determination of 1-deoxynojirimycin in mulberry leaves", Biofactors, 22, pp.341~345 (2004)
- Stein DC, "Characterization of Bacillus subtilis DSM704 and its production of 1-deoxynojirimycin", Appl. Environ. Microbiol, 8, pp.280~284 (1984)
- Schmidt DD, "Glucosidase inhibitiors from Bacilli", Naturwissenschaften, 66, pp.584~585 (1979)
- Murao S, "Isolation and characterization of a new trehalase inhibitor, S-GI", Agric. Biol. Chem, 44, pp.219~221 (1980)
- Ezure Y, "Moranoline(1-deoxynojirimycin) fermentation and its improvement", Agric. Biol. Chem, 49, pp.1119~1125 (1985)
- Chen HM, "A new method for screening alpha-glucosidase inhibitors and application to marine microorganisms", Pharm. Biol, 42, pp.416~421 (2004)
- Paek NS, "Production of 1-deoxynojirimycin by Streptomyces sp. SID9135", J. Microbiol. Biotechnol, 7, pp.262~266 (1997)
- Haaland PD., "Experimental design in biotechnology" (1989), Dekker
- Box GEP, "On the experimental attainment of optimum condition", J. Roy. Stat. Soc. B, 13, pp.1~45 (1951)
- Lim JS, "Optimization of culture medium and conditions for neofructooligosaccharides production by Penicillium citrinum", Eur. Food Res. Technol, 221, pp.639~644 (2005)
- Vichasilp C, "Optimization of 1-deoxynojirimycin extraction from mulberry leaves by using response surface methodology", Biosci. Biotechnol. Biochem, 73, pp.2684~2689 (2009)
- Kalil SJ, "Response surface analysis and simulation as a tool for bioprocess design and optimization", Process Biochem, 35, pp.539~550 (2000)
- 김현수, "알파글루코시다아제 저해제 1-deoxynojirimycin을 생산하는 Bacillus 균주의 분리 및 동정", 한국미생물·생명공학회지, 39(1), pp.49~55 (2011), 한국미생물·생명공학회
- Montgomery DC., "Design and analysis of experiments" (1991), Wiley
- Matsui T, "α-glucosidase inhibitory action of natural acylated anthocyanins. 1. Survey of natural pigments with potent inhibitory activity", J. Agric. Food Chem, 49, pp.1948~1951 (2001)
- Kim JW, "Determination of 1-deoxynojirimycin in Morus alba L. leaves by derivatization with 9-fluorenylmethyl chloroformate followed by reversedphase high-performance liquid chromatography", J. Chromatogr. A, 1002, pp.93~99 (2003)
- Kada S, "Identification of two major ammonia-releasing reactions involved in secondary fermentation", Biosci. Biotechnol. Biochem, 72, pp.1869~1876 (2008)
- Hei Chan Lee, "Statistical Optimization of Medium Components for the Improved Production of Cystocin by Streptomyces sp. GCA0001", Journal of Industrial and Engineering Chemistry, 8(5), pp.427~431 (2002), 한국공업화학회
- Yatsunami K, "The relationship between 1-deoxynojirimycin content and α-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto, Japan", J. Nat. Med, 62, pp.63~66 (2008)
- Zhu YP, "Purification and identification of 1-deoxynojirimycin (DNJ) in okara fermented by Bacillus subtilis B2 from Chinese traditional food (Meitaoza)", J. Agric. Food Chem, 58, pp.4097~4103 (2010)
- Pavlova K, "Production and properties of exopolysaccharide by Rhodotorula acheniorum MC", Food Res. Int, 32, pp.473~477 (1999)