배양기의 작동인자가 Thraustochytrium aureum ATCC 34304의 성장 및 Docosahexaenoic acid(DHA)의 생합성에 미치는 영향규명
Effect of Operating Factors on the Growth and DHA Biosynthesis of Thraustochytrium aureum ATCC34304
1
조회
0
다운로드
0
인용
0
참고문헌
초록
교반속도와 배양온도가 T. aureum ATCC 34304의 성장과 지질 및 DHA 생합성에 미치는 영향을 규명하였다. 배양온도 4oC 내지 39oC 범위에서 균체성장에 최적인 온도는32oC이었으며, DHA와 지질의 생성량이 가장 높은 온도는18oC이였다. 배양온도 39oC 이상과 11oC이하에서는 균체의성장량과 DHA 및 지질의 생성량이 대단히 적었으나, 온도가 낮을수록 단위균체당 축적되는 지질과 DHA의 함량은 증가하여 배양온도 4oC에서는 YL/X의 값이 32%까지, YDHA/X의 값은 15%까지 증가하였다.교반속도 50 내지 250 rpm 사이에서 균체성장이 가장 높은 교반속도는 100 rpm이였다. 교반속도 100과 250 rpm 사이에서는 교반속도가 균체의 성장량과 지질 및 DHA 생합성량에는 영향을 미치지 아니하였으나, 교반속도가 50 rpm으로 감소하면 균체 성장량과 지질 및 DHA 생성량이 모두크게 감소하였다.
Abstract
The effect of rotation speed and culture temperature on the growth of T. aureum and also the biosynthesis of lipid and DHA was investigated. The optimal temperature for the growth was 32oC, but the best temperature for the maximum production of lipid and DHA was 18oC in the range of 4oC to 39oC. In the case that the temperature was higher than 39oC and lower than 11oC, the growth was very slow and the production of DHA was also very low. However, the lipid content in the biomass became higher with decrease in the culture temperature. The rotation speed for the maximum growth was 100 rpm. But the growth and the production of DHA were not affected by the change of rotation speed in the range of 100 to 250 rpm. When the rotation speed was 50 rpm, the growth as well as the production of DHA and lipid was greatly reduced.
참고문헌 (17)
- "Optimizationof production of docosahexaenoic acid", pp.509~68 514 (1991)
- "Dietary ploysaturatedfat in relation to mammorial carcinogenesis in rats", pp.285~288 (1986)
- "Arachidonic acid status of humaninfants influence of gestational age at birth and diets withvery long chain n-3 and n-6 fatty acids", 126 (1996)
- "Fattyacid composition of Spirulina strains grown under variousenvironmental conditions", pp.2255~2258 (1987)
- "Health effects of polyunsaturatedfatty acid in seafoods", pp.319~330 (1986), Academic PressInc
- "Linolenate-derived polyunsaturated fattyacids and prevention of Atherosclerosis", pp.125~134 (1986)
- "Plasma lipoprotein profile and lipolytic activitiesin response to the substitution of lean white fish for otheranimal protein sources in premenopausal women", 63, pp.315~321 (1996)
- "Effects of medium glucose concentrationand pH on docosahexaenoic acid content of heterotrophicCrypthecodinium cohnii", 35, pp.1205~1209 (2000)
- "Effect of temperature on lipid unsaturation", 5, pp.245~268 (1987)
- "Toomer", 13, pp.123~131
- "Arachidonic acidfrom fungi utilizing fatty acids with short chains as solesources of carbon and energy", pp.387~393 (1988)
- "Arachidonicacid production by Mortierella sp", dobro(va), pp.455~17 456 (1990)
- "Health effects of polyunsaturatedfatty acids in seafoods", pp.49~60 (1986), Academic PressInc
- "Effectof cultural conditions on production of eicosapentaenoic acidby Pythium irregulare", pp.171~178 (1991)
- "microbial production of long-chainPUFAs", 6, pp.683~688 (1995)
- "Alternative sources of production", 24, pp.117~125 (1989)
- "Optimization of docosahexaenoic acid production bySchizochytrium limacium SR21", 49, pp.72~76