토마토시들음병의 생물학적 방제를 위한 토착길항세균 Bacillus thuringiensis BK4의 선발과 길항기작
Selection and Antagonistic Mechanism of Bacillus thuringiensis BK4 against Fusarium Wilt Disease of Tomato.
2
조회
0
다운로드
5
인용
0
참고문헌
초록
토마토시들음병의 원인균인 Fusarium oxysporum의 생물학적 방제를 위해 경북지역의 저병해경작지로부터 항진균성 항생물질 생산성 길항미생물인 BK4 균주를 선발하였으며, 길항균주 BK4의 16s rDNA 분석 및 생화학적 특성 조사 후 최종적으로 Biolog사 동정시스템인 MicroLogTM 3.0을 이용하여 선발 길항균주 BK4를 Bacillus thuringiensis BK4로 동정하였다. B. thuringiensis BK4는 pH 9.0로 적정한 Nutrient broth를 이용하여 30oC에서 배양할 경우 항생물질의 생산은 최대였다. B. thuringiensis BK4가 생산하는 항생물질은 121oC에서 열처리시에도 80% 잔존활성을 유지한 내열성 항생물질이었으며, pH 3.0~12.0의 범위에서 활성의 변화를 조사한 결과 산성보다는 알칼리성에서 다소 안정적이였으나 pH 3.0에서도 50% 이상의 잔존 활성을 가지고 있다. B. thuringiensis BK4는 F. oxysporum에 의한 토마토시들음병에 대하여 in vivo pot상에서도 충분한 방제력을 나타내었으므로 토마토시들음병을 위시한 Fusarium속 균주가 원인이 되는 질병을 방제할 수 있는 미생물제제의 개발이 가능하게 되었다.
Abstract
In oder to select an antifungal substance-producing antagonistic bacterium against Fusarium oxysporum casuing fusarium wilt on tomato, strain BK4 was isolated from local soil of Gyeoungbuk and was identified as Bacillus thuringiensis by 16S rDNA analysis, biochemical test, and Mcirolog TM 3.0 System. The antibiotic of B. thuringiensis BK 4 was highly produced at 30oC in nutrient broth (pH 9.0). The crude antibiotic was even stable at 121oC and more stable at slight alkalic condition than acid condition. It was also remained 50% activity at pH 3.0. B. thuringiensis BK4 showed the inhibition of spore germination and the biocontrol ability against F. oxysporum causing fusarium wilt of tomato in vivo test. According to these results, B. thuringiensis BK4 was enough to use with a microbial agent for biocontrol against fusarium wilt.
참고문헌 (12)
- "Biological control with Streptomyces sp", pp.231~238 (1991)
- "Effect of a soil amendment for controling fusarium wilt of cucumber caused by Fusarium oxysporum f", 24, pp.93~104 (1996)
- "Bergey's Manual of Systematic Bacteriology" (1994)
- "Selection and antagonistic mechanism of Pseudomonas fluorescens 4059 against phytophthora blight disease", 32, pp.312~316 (2004)
- "Antifungal activities of Metarhizium anisopliae against Fusarium oxysporum", 24, pp.49~55 (1996)
- "Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtilis YB-70 as a biocontrol agent", pp.296~304 (1994)
- "The mechanism of biological control of Pseudomonas spp", 23, pp.91~98 (1995)
- "Isolation and antifungal activity of the chitinase producing bacterium Serratia sp 3095 as antagonistic bacterium against Fusarium sp", 42
- "and bioassay of suppression of fusarium wilt of radish", 25, pp.10~20
- "The role and characterization of Β-1 3-glucanase in biocontrol of Fusarium solani by Pseudomonas stutzeri YPL-1", pp.295~301 (1995)
- "The Production and enzymatic properties of extracellular chitinase from Pseudomonas stutzeri YPL-1", pp.134~140 (1994)
- "Selection of non-pathogen Fusarium oxysporum for biocontrol against Fusarium oxysporum f" (1993)