Production of methionine γ- lyase in recombinant Citrobacter freundii bearing the hemoglobin gene
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The production of antileukemic enzyme methionine γ-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine γ- liyase production. Methionine γ- liyase production by Citrobacter freundii and its vgb− and vgb＋ bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30oC in a time-course manner. The vgb＋ and especially the carbon type had a dramatic effect on methionine γ- liyase production. The vgb+ strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb− strain, respectively. [BMB reports 2011; 44(9): 590-594]
Abstract
The production of antileukemic enzyme methionine γ-lyase (MGL) in distinctly related bacteria, Citrobacter freundii and in their recombinants expressing the Vitresocilla hemoglobin (VHb) has been studied. This study concerns the potential of Citrobacter freundii expressing the Vitreoscilla hemoglobin gene (vgb) for the methionine γ- liyase production. Methionine γ- liyase production by Citrobacter freundii and its vgb− and vgb＋ bearing recombinant strain was studied in shake-flasks under 200 rpm agitation, culture medium and 30oC in a time-course manner. The vgb＋ and especially the carbon type had a dramatic effect on methionine γ- liyase production. The vgb+ strain of C. freundii had about 2-fold and 3.1-fold higher levels of MGL than the host and vgb− strain, respectively. [BMB reports 2011; 44(9): 590-594]
참고문헌 (22)
- Geckil, H, "Effect of Vitreoscilla hemoglobin on production of a chemotherapeutic enzyme, L-asparaginase, by Pseudomonas aeruginosa", Biotechnol. J, 1, pp.203~208 (2006)
- Takakura, T, "High-level expression and bulk crystallization of recombinant L-methionine γ-lyase, an anticancer agent", Appl. Microbiol. Biotechnol, 70, pp.183~192 (2006)
- Takakura, T, "Assay method for antitumor L-methionine γ-lyase: comprehensive kinetic analysis of the complex reaction with L-methionine", Anal. Biochem, 327, pp.233~240 (2004)
- Weimer, B, "Sulfur metabolism in bacteria associated with cheese", Anton. Leeuw, 76, pp.247~261 (1999)
- Manukhov, I. V, "L-Methionine γ-lyase from Citrobacter freundii: cloning of the gene and kinetic parameters of the enzyme", Biochem, 71, pp.361~369 (2006)
- Nikulin, A, "Highresolution structure of methionine γ-lyase from Citrobacter freundii", Acta Cryst, 64, pp.211~218 (2008)
- Kudou, D, "Structure of the antitumour enzyme L-methionine γ-lyase from Pseudomonas putida at 1.8A° Resolution", J. Biochem, 141, pp.535~544 (2007)
- Manukhov, I. V, "A gene encoding L-methionine γ-lyase is present in Enterobacteriaceae family genomes: Identification and characterization of Citrobacter freundii L-methionine γ-lyase", J. Bacteriol, 187, pp.3889~3893 (2005)
- Mamaeva, D. V, "Structure of Citrobacter freundii L-methionine γ-lyase", Acta Cryst. Section F, 61, pp.546~549 (2005)
- Yoshimura, M, "L-Methionine- γ-lyase, as a target to inhibit malodorous bacterial growth by trifluoromethionine", Biochem. Bioph. Res. Co, 292, pp.964~968 (2002)
- Coombs, G. H, "Trifluoromethionine, a prodrug designed against methionine γ-lyase-containing pathogens, has efficacy in vitro and in vivo against Trichomonas vaginalis", Antimicrob. Agents. Chem, 45, pp.1743~1745 (2001)
- Kokkinakis, D. M, "Effect of long-term depletion of plasma methionine on the growth and survival of human brain tumor xenografts in athmic mice", Nutr. Cancer, 29, pp.195~204 (1997)
- Takakura, T, "Physicochemical and pharmacokinetic characterization of highly potent recombinant L-methionine γ-lyase conjugated with polyethylene glycol as an antitumor agent", Cancer Res, 66, pp.2807~2814 (2006)
- Lishko, V. K, "Depletion of serum methionine by methioninase in mice", Anticancer Res, 13, pp.1465~1468 (1993)
- Borenshtein, D, "The genus Citrobacter", Prokaryotes, 6, pp.90~98 (2006)
- Wang, J, "Comparison of antimicrobial susceptibility of Citrobacter freundii isolates in two different time periods", 33, pp.258~262 (2000)
- Geckil, H, "Genetic engineering of Enterobacter aerogenes with Vitreoscilla hemoglobin gene: cell growth, survival, and antioxidant enzyme status under oxidative stress", Res. Microbiol, 154, pp.425~431 (2003)
- Geckil, H, "Cell growth and oxygen uptake of Escherichia coli and Pseudomonas aeruginosa are differently affected by the genetically engineered Vitreoscilla hemoglobin gene", J. Biotechnol, 85, pp.57~66 (2001)
- Yang, J, "ArcA Works with Fnr as a positive regulator of Vitreoscilla (bacterial) hemoglobin gene expression in Escherichia coli", Microbiol. Res, 160, pp.405~415 (2005)
- Kurt, A. G, "Production of L- DOPA and dopamine in recombinant bacteria bearing the Vitreoscilla hemoglobin gene", Biotechnol. J, 4, pp.1077~1088 (2009)
- Tanaka, H, "Selective determination of L-methionine and L-cysteine with bacterial L-methionine γ-lyase and anti-tumor activity of the enzyme", J. Appl. Biochem, 2, pp.439~444 (1980)
- Soda, K, "Microdetermination of D-amino acids and D-amino acid oxidase activity with 3-methyl-2-benzothiazolone hydrazone hydrochloride", Anal. Biochem, 25, pp.228~235 (1968)