산약 추출물의 항산화 및 항염증에 관한 실험적 연구
Experimental Study on the Antioxidant and Antimicrobial Properties of Dioscoreae Rhizoma
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Objectives : This study evaluated the effect of Dioscoreae Rhizoma on antioxidant and antimicrobial activity in vitro. Methods : In vitro experiments were made on antioxidant by DPPH radical scavenging activity, enzyme activity of SOD, protective effect about H2O2 and DNA fragmentation in RAW264.7 cells, as well as antimicrobial by Cox-2 expression in LPS- induced RAW264.7 cells. Results :1. Dioscoreae Rhizoma's antioxidant ability was improved by DPPH radical scavenging activity. 2. Antioxidant ability of Dioscoreae Rhizoma produced good results at more than 0.025 ㎎/㎖. 3. On protective effect of Dioscoreae Rhizoma about H2O2 on RAW264.7 cells, with addition of Dioscoreae Rhizoma extract the cells' survival increased compared to adding H2O2 only. 4. About revelation-control of Cox-2 at RAW264.7 cells which induce by LPS, when Dioscoreae Rhizoma's concentration was higher from 0.0125 ㎎/㎖ to 1 ㎎/㎖, its control activity was stronger. Thus we found Dioscoreae Rhizoma has antimicrobial activity. 5. The power of DNA fragmentation control of Dioscoreae Rhizoma was time-independent. Conclusions : From this study we conclude that Dioscoreae Rhizoma has effects on antioxidant and antimicrobial activity. However, considerable work still needs to be done studying antioxidants by concentration, as well as a variety of experiments about antimicrobial activity.
Abstract
Objectives : This study evaluated the effect of Dioscoreae Rhizoma on antioxidant and antimicrobial activity in vitro. Methods : In vitro experiments were made on antioxidant by DPPH radical scavenging activity, enzyme activity of SOD, protective effect about H2O2 and DNA fragmentation in RAW264.7 cells, as well as antimicrobial by Cox-2 expression in LPS- induced RAW264.7 cells. Results :1. Dioscoreae Rhizoma's antioxidant ability was improved by DPPH radical scavenging activity. 2. Antioxidant ability of Dioscoreae Rhizoma produced good results at more than 0.025 ㎎/㎖. 3. On protective effect of Dioscoreae Rhizoma about H2O2 on RAW264.7 cells, with addition of Dioscoreae Rhizoma extract the cells' survival increased compared to adding H2O2 only. 4. About revelation-control of Cox-2 at RAW264.7 cells which induce by LPS, when Dioscoreae Rhizoma's concentration was higher from 0.0125 ㎎/㎖ to 1 ㎎/㎖, its control activity was stronger. Thus we found Dioscoreae Rhizoma has antimicrobial activity. 5. The power of DNA fragmentation control of Dioscoreae Rhizoma was time-independent. Conclusions : From this study we conclude that Dioscoreae Rhizoma has effects on antioxidant and antimicrobial activity. However, considerable work still needs to be done studying antioxidants by concentration, as well as a variety of experiments about antimicrobial activity.
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