항생물질을 생산하는 Pseudomonas sp. BCNU 2001 균주의 특성
Characterization of an Antimicrobial Substance-producing Pseudomonas sp. BCNU 2001.
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본 연구팀은 태백산 지역에서 토양 시료들을 채취하여 다양한 균주들을 분리 탐색하는 과정에서 BCNU 2001 균주를 분리하였다. 분리균주의 생화학적 특징과 16S 리보좀RNA 유전자 염기서열 분석 결과, 분리균주가 Pseudomonas aeruginosa에 속함이 확인되었다. 분리균주 BCNU 2001의상등액은 다양한 세균과 진균에 대해 항균활성이 있었으며특히 분리균주 BCNU 2001는 Micrococcus luteus, Proteus mirabilis, Proteus vulgaris, 그리고 Aspergillus niger의 생육을 크게 저해할 수 있었으며, Micrococcus luteus, Proteus mirabilis, Proteus vulgaris, 그리고 Aspergillus niger에 대하여 각각 18.5 mm, 19.0 mm, 17.0 mm 그리고 13.5 mm 크기의 저해환을 나타내었다. 분리균주 BCNU 2001의 핵산분획물과 디클로로메탄 분획물은 세균에 대해 높은 항균력을 보였으며 디클로로메탄 분획물과 에틸아세테이트 분획물은 진균에 대해 높은 항균력을 보였다. 또한 Pseudomonas sp. BCNU 2001 균주는 그람양성 세균, 그람음성 세균 그리고 진균을 포함한 다양한 미생물들에 대하여 항균력이 있는항균 펩타이드를 가지고 있음이 확인되었다. 실험을 통하여얻은 결과는 Pseudomonas sp. BCNU 2001 균주의 유용성에 대한 예비적인 기초를 제공한다.
Abstract
Strain BCNU 2001 was isolated from soil samples collected from Tea-baek Mountain area. The biochemical characteristics and 16S ribosomal RNA gene sequences of the isolate revealed that the strain belonged to the Pseudomonas aeruginosa. The supernatants had an antimicrobial effect on various kind of bacteria and fungi. Especially BCNU 2001 was able to greatly inhibit the growth of Micrococcus luteus, Proteus mirabilis, Proteus vulgaris, and Aspergillus niger, and its inhibition zone was measured as 18.5 mm against Micrococcus luteus, 19.0mm against Proteus mirabilis, 17.0mm against Proteus vulgaris, and 13.5mm against Aspergillus niger, respectively. Hexane and dichloromethane extracts of BCNU 2001 exhibited significant activity against bacteria, and dichloromethane and ethylacetate extracts showed significant activity against fungi. Pseudomonas strain BCNU 2001 was also determined to have antimicrobial peptide against various microorganisms including Gram positive bacteria, Gram negative bacteria and fungi. The obtained results may provide preliminary support for the usefulness of Pseudomonas strain BCNU 2001.
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