Pseudomonas otitidis PS 균주의 항생물질 생산과 작용 기작
Antibiotic Production of Pseudomonas otitidis PS and Mode of Action
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Gram 양성세균의 생육을 억제하는 세균을 청주시 무심천토양에서 분리하였으며, 16S rRNA 유전자 염기서열 분석 결과 Pseudomonas otitidis PS로 동정하였다. PS 균주는 0.5% 의 glucose가 포함된 1% soybean meal 배지에서 2차 대사 산물로서 최대 약 0.1%의 수율로 항생물질을 생산하였다. 항생물질 성분은 ethyl acetate로 추출하였으며, Staphylococcus aureus KCTC 1261에 대한 minimum inhibitory concentration은2 μg/ml이었다. 이 성분은 siderophore 활성을 띠어서 chrome azurol S 평판배지에서 주황색 halo를 나타내었으며, 철이 제거되면 생육 억제 효과는 감소하였다. Ascorbic acid 같은 환원제를 첨가하거나 혐기적 환경에서는 항생물질 활성을 잃으므로 PS 항생물질은 활성산소를 방출하여bactericidal activity를 갖는 것으로 보인다.
Abstract
An isolate capable of inhibiting the growth of gram-positive bacteria was obtained from the soil of Mushim stream, Cheongju. The isolate was identified as Pseudomonas otitidis PS by 16S rRNA gene sequence analysis. P. otitidis PS produced antibiotics as a secondary metabolite when cultured in 1% soybean meal with 0.5% glucose. The maximum yield was about 0.1%. The antibiotic substance of P. otitidis PS extracted using ethyl acetate displayed a minimum inhibitory concentration of 2 μg/ml for Staphylococcus aureus KCTC 1261. The antibiotic substance produced an orange halo on chrome azurol S agar due to siderophore activity. Growth inhibition was decreased when the iron was depleted. Since the antibiotic activity was lost upon the addition of the reducing agent ascorbic acid or during anaerobic culture, it was considered that antibiotic of P. otitidis PS strain exerts its bactericidal effect by the generation of reactive oxygen species.
참고문헌 (26)
- Baron SS, "Antibiotic action of pyocyanin", Antimicrob. Agent. Chemother., 20, pp.814~820 (1981)[DOI]
- Linda ST, "Production of the antibiotic phenazin-1- carboxylic acid by fluorescent Pseudomonas species in the rhizosphere of wheat", Appl. Environ. Microbiol., 56, pp.908~912 (1990)
- Arima K, "Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas", Agric. Biol. Chem., 28, pp.575~576 (1964)[DOI]
- Howell CR, "Suppression of Pythium ultimum- induced damping-off of cotton seedlings by Pseudomonas fluorescens and its antibiotics, pyoluteorin", Phytopathology, 70, pp.712~715 (1980)[DOI]
- Cox CD, "Pyochelin: Novel structure of an iron-chelating growth promoter for Pseudomonas aeruginosa", Pro. Natl. Acad. Sci. USA, 78, pp.4256~4260 (1981)[DOI]
- Shanahan P, "Isolation of 2,4-diacetylphloroglucinol from a fluorescent pseudomonad and investigation of physiological parameters influencing its production", Appl. Environ. Microbiol., 58, pp.353~358 (1992)
- Vandenbergh PA, "Ironchelating compounds produced by soil Pseudomonas: Correlation with fungal growth inhibition", Appl. Environ. Microbiol., 46, pp.128~132 (1983)
- Cornelis P, "Diversity of siderophore-mediated iron uptake systems in fluorescent pseudomonads: not only pyoverdines", Environ. Microbiol., 4, pp.787~798 (2002)
- Sheldon JR, "Recent developments in understanding the iron acquisition strategies of Gram positive pathogens", FEMS Microbiol. Rev., 39, pp.592~630 (2015)
- Endicott NP, "Structural basis for xenosiderophore utilization by the human pathogen Staphylococcus aureus", ACS Infect. Dis., 3, pp.542~553 (2017)[DOI]
- Philson SB, "Siderochromes from Pseudomonas fluorescens", J. Biol. Chem., 257, pp.8081~8085 (1982)
- Cox CD, "Siderophore activity of pyoverdine for Pseudomonas aeruginosa", Infect. Immun., 48, pp.130~138 (1985)
- Ankenbauer RG, "Synthesis and biological activity of pyochelin, a siderophore of Pseudomonas aeruginosa", J. Bacteriol., 170, pp.5344~5351 (1988)[DOI]
- Adler C, "Catecholate siderophore protect bacteria from pyochelin toxicity", PLoS One, 7, pp.e46754~ (2012)[DOI]
- Clark LL, "Psedomonas otitidis sp. nov., isolated from patients with otic infections", Int. J. Syst. Evol. Microbiol., 56, pp.709~714 (2006)[DOI]
- 상미경, "Biocontrol of Phytophthora Blight and Anthracnose in Pepper by Sequentially Selected Antagonistic Rhizobacteria against Phytophthora capsici", The Plant Pathology Journal, 29(2), pp.154~167 (2013), 한국식물병리학회[DOI]
- Wegner DL, "Direct method to determine the antibiotic susceptibility of rapid growing blood pathogens", Antimicrob. Agent. Chemother., 9, pp.861~862 (1976)[DOI]
- Leisinger T, "Secondary metabolites of the fluorescent pseudomonads", Microbiol. Rev., 43, pp.422~442 (1979)
- Miethke M, "Siderophore-based iron acquisition and pathogen control", Microbiol. Mol. Biol. Rev., 71, pp.413~451 (2007)[DOI]
- Schwyn B, "Universal chemical assay for the detection and determination of siderophores", Anal. Biochem., 160, pp.47~56 (1987)[DOI]
- Reeves M, "Absence of siderophore activity in Legionella sp. grown in iron deficient media", J. Bacteriol., 154, pp.324~329 (1983)
- Gillam A, "Quantitative determination of hydroxamic acid", Anal. Chem., 53, pp.841~844 (1981)
- Roosenberg JM, "Studies and syntheses of siderophore, microbial iron chelators, and analogs as potential drug delivery agents", Curr. Med. Chem., 7, pp.159~197 (2000)[DOI]
- Mollman U, "Siderophores as drug delivery agents: application of the "Trojan Horse" strategy", Biometals, 22, pp.615~624 (2009)[DOI]
- Braun V, "Sideromycin: tools and antibiotics", Biometals, 22, pp.3~13 (2009)[DOI]
- Ji C, "Iron Transport-mediated drug delivery: Practical syntheses and in vitro antibacterial studies of tris-catecholates siderophore-aminopenicillin conjugates reveals selectively potent anti-pseudomonal activity", J. Am. Chem. Soc., 134, pp.9898~9901 (2012)[DOI]