갯메꽃 뿌리로부터 분리된 내생진균의 식물생장촉진활성
Plant Growth Promotion Activity of Endophytic Fungi Isolated from the Roots of Calystegia soldanella
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초록
서해안에 자생하는 갯메꽃의 뿌리로부터 8종의 내생진균을 분리하였다. 그리고 식물생장촉진검정을 확인하기 위하여 분리된 내생진균의 배양여액을 난장이벼에 처리하였다. Cs-9-7 균주의 배양여액 처리구에서 난장이벼의 생장촉진활성이 가장 높았다. 그리고 Cs-9-7 균주의 배양여액은 GC/MS-SIM을 사용하여 이차대사산물을 분석하였을 때, GA₃(1.229 ng/mL), GA₄(3.535 ng/mL), GA_7(1.408 ng/mL) 그리고 GA_12(0.378 ng/mL)의 지베렐린이 확인되었다. 그리고분리된 내생진균의 동정을 위하여, 유니버샬 프라이머 ITS1과 ITS4를 사용하여 ITS 영역의 염기서열을 결정하였다. Cs-9-7 균주의 동정결과 A. tubingensis와 99%의 유사성이 확인되었으며, A. tubingensis Cs-9-7라고 명명하였다.
Abstract
Eight endophytic fungal strains were isolated from the roots of Calystegia soldanella from the western coast of South Korea. The culture filtrate of the eight endophytic fungi were applied to waito-c rice seedlings in order to verify potential plant growth promotion activities. The results of bioassay indicated that the Cs-9-7 fungal strain possessed the highest plant growth promotion activity. Fungal culture filtrates were analyzed to verify secondary metabolites using gas chromatography and mass spectroscopy with selected ion monitoring (GC/MS-SIM). The culture filtrate of the Cs-9-7 fungal strain was confirmed to contain gibberellins GA3 (1.229 ng/mL), GA4 (3.535 ng/mL), GA7 (1.408 ng/mL) and GA12 (0.378 ng/mL). Polymerase chain reactions (PCR) were performed so as to determine the internal transcribed spacer (ITS) regions for the identification of isolated strains with universal primers ITS-1 and ITS-4. The Cs-9-7 fungal strain, isolated from the root of C. soldanella, has been named Aspergillus tubingensis Cs-9-7.
참고문헌 (22)
- Basiacik, K. S, "Optimization of carbonnitrogen ratio for production of gibberellic acid by Pseudomonassp", Pol. J. Microbiol, 53, pp.20~117 (2004)
- Bottini, R., "Gibberellinproduction by bacteria and its involvement in plant growthpromotion and yield increase", Appl. Microbiol. Biotechnol, 65, pp.497~503 (2004)
- Carter,R.W.G, "Near future sea level impacts oncoastal dunes landscapes", Landscape Ecol, 6, pp.29~39 (1991)
- 김종국, "생강나무와 산앵두나무의 뿌리에서 분리한 Penicillium spp.의 지배렐린 생산성", 한국균학회지, 32(1), pp.16~22 (2004), 한국균학회
- Garia-Mora, M. R., "Plant diversity as a suitable tool for coastaldune vulnerability assessment", J. Coastal Res, 16, pp.990~995 (2000)
- Hasan, H. A, "Gibberellin and auxin-production byplant root-fungi and their biosynthesis under salinity-calciuminteraction", Acta Microbiol. Immunol. Hung, 49, pp.105~118 (2002)
- 강상모, "Gibberellin production by pure cultures of a new strain of Aspergillus fumigatus", World Journal of Microbiology and Biotechnology, 25(10), pp.1785~1792
- Hedden, P, "Gibberellin metabolism:new insights revealed by the genes", Trands in Plant Sci, 5, pp.523~530 (2000)
- Hwang, J. S., "Effects of Endophytic Fungal Secondary Metabolites on the Growth and Physiological Response of Carex kobomugi Ohwi", JOURNAL OF COASTAL RESEARCH, 27(3), pp.544~548 (2011), COASTAL EDUCATION RESEARCH FOUNDATION
- Kawaide, H, "Accumulation of gibberellinA1 and the metabolism of gibberellin A9 to gibberellin A1 ina Phaeosphaeria sp. L 487 culture", Biosci. Biotech. Biochem, 57, pp.1403~1405 (1993)
- Khan, S. A., "Gibberellin production and plantgrowth promotion by a newly isolated strain of Gliomastixmurorum", World J. Microbiol. Biotechnol, 25, pp.829~833 (2009)
- Khan, S. A., "A new strain of Arthrinium phaeospermum isolatedfrom Carex kobomugi Ohwi is capable of gibberellinproduction", Biotechnol. Lett, 31, pp.283~287 (2009)
- Khan, S. A., "Plant growthpromotion and Penicillium citrinum", BMC Microbiol, 8, pp.231~ (2008)
- Kawanabe, Y., "Identification of gibberellin A3 inmycelia Neurospora Crassa", Agric. Biol. Chem, 47, pp.1693~1694 (1983)
- KIM, "Remarkable Bacterial Diversity in the Tidal Flat Sediment as Revealed by16S rDNA Analysis", Journal of Microbiology and Biotechnology, 14(1), pp.205~211 (2004), 한국미생물·생명공학회
- Lee, I. J., "Photoperiodcontrol of gibberellin levels and flowering in sorghum", Plantphysiol, 116, pp.1003~1011 (1998)
- Opelt, K, "Diversity and antagonisticpotential of bacteria associated with bryophytes fromnutrient poor habitats of baltic sea coast", Appl. Microbiol, 70, pp.6569~6579 (2004)
- Rachev, R., "Gibberellin biosynthesis by Fusariummoniliforme in the presence of hydrophobic resin AmberliteXAD-2", Bulg. J. Plant Physi, 12, pp.24~31 (1997)
- RIM, "Fusarium proliferatum KGL0401 as a New Gibberellin-Producing Fungus", Journal of Microbiology and Biotechnology, 15(4), pp.809~814 (2005), 한국미생물·생명공학회
- Vazquez, M. M., "Interactionbetween arbuscular mycorrhizal fungi and othermicrobial inoculants (Azospirillum, Pseudomonas, Trichoderma)and their effects on microbial population andenzyme activities in the rhizosphere of maize plants", Appl.Soil Ecol, 15, pp.261~272 (2000)
- Williams,A.T, "Integrated management methodsmonitoring environmental changes in coastal dune ecosystem", pp.642~653 (1998), Brusells
- Yamada, A., "Isolationof Tricholoma matsutake and T. bakamatsutake culturesfrom field-collected ectomycorrhizas", Mycoscience, 42, pp.43~50 (2001)