신두리 해안사구에 자생하는 사구식물 내생진균의 다양성 분석
Endophytic Fungal Diversity Associated with the Roots of Coastal Sand-dune Plants in the Sindu-ri Coastal Sand Dune, Korea.
2
조회
0
다운로드
7
인용
0
참고문헌
키워드
초록
98주의 내생진균은 신두리 해안사구에 자생하고 있는 해안 사구식물의 뿌리로부터 분리하였다. 8종의 해안사구식물 샘플은 모래지치(Argusia sibirica), 갯메꽃(Calystegia soldanella), 갯그령(Elymus mollis), 반디지치(Lithospermum zollingeri), 갯무(Raphanus sativus), 솔장다리(Salsola collina), 왕잔디(Zoysia macrostachya) 및 갯잔디(Zoysia sinica)이며, 신두리 해안사구로부터 채집되었다. 그리고 분리된 내생진균들은 ITS1, 5.8S와 ITS2를 포함하는 ITS-rDNA 영역에 의해 분석되었다. 해안사구식물로부터 분리된 내생진균에 대하여 다양한 지수를 적용하여 분석하였다. 해안사구식물로부터 분리된 모든 내생진균은 Capnodiales (3.09%), Eurotiales(70.10%), Glomerellales (1.03%), Helotiales (3.09%), Hypocreales (9.28%), Mortierellales (2.06%), Onygenales(1.03%), Ophiostomatales (1.03%), Pleosporales (1.03%), Polyporales (1.03%), Russulales (1.03%), Saccharomycetales(2.06%), Xylariales (1.03%)로 13개 목과 분류체계가 명확하지 않은 Incertae sedis (3.09%)으로 확인되었다. 본 연구에서는 8종의 식물로부터 내생진균을 분석한 결과 Eurotiales 목과 Hypocreales 목의 Penicillium 속(59.18%)과 Fusarium속(5.10%)이 가장 많이 분포하는 것으로 나타났다. 갯메꽃으로부터 분리된 내생진균이 다른 해안사구식물들로부터 분리된 내생진균의 다양성 보다 높은 것으로 확인되었다.
Abstract
The coastal sand-dune plants of eight species; Argusia sibirica, Calystegia soldanella, Elymus mollis, Lithospermum zollingeri,Raphanus sativus, Salsola collina, Zoysia macrostachya, and Zoysia sinica were collected from the Shindu-ri coastal sand dune. Ninety-eight endophytic fungal strains were isolated from the roots of these plants, analyzed, and identified by sequences in their internal transcribed spacers (ITS) at the ITS1, 5.8S, and ITS2 regions. The diversity of endophytic fungi isolated from coastal sand-dune plants was confirmed with various diversity indices. The fungal strains belonged to thirteen orders: Capnodiales (3.09%), Eurotiales (70.10%), Glomerellales (1.03%), Helotiales (3.09%), Hypocreales (9.28%), Mortierellales (2.06%), Onygenales (1.03%), Ophiostomatales (1.03%), Pleosporales (1.03%), Polyporales (1.03%), Russulales (1.03%), Saccharomycetales (2.06%), and Xylariales (1.03%). Of the endophytic fungal strains collected, Penicillium (59.18%in Eurotiales) and Fusarium (5.10% in Hypocreales) were the most abundant in coastal sand-dune plants. The endophytic fungal strains isolated from C. soldanella were more diverse compared to strains from the other coastal sand-dune plants.
참고문헌 (25)
- Carter RWG, "Near future sea level impacts on coastal dunes landscapes", Landscape. Ecol, 6, pp.29~39 (1991)
- Fisher RA, "The relation between the number of species and the number of individuals in a random sample of an animal population", J. Anim. Ecol, 12, pp.42~58 (1943)
- Garia-Mora MR, "Plant diversity as a suitable tool for coastal dune vulnerability assessment", J. Coastal Res, 16, pp.990~995 (2000)
- Hasan HA, "Gibberellin and auxin-production by plant root-fungi and their biosynthesis under salinity-calcium inter-action", Acta Microbiol. Immunol. Hung, 49, pp.105~118 (2002)
- Hwang JS, "Effects of endophytic fungal secondary metabolites on the growth and physiological response of Carex kobomugi Ohwi", J. Coastal. Res, 27, pp.544~548 (2011)
- Khan SA, "Gibberellin production and plant growth promotion by a newly isolated strain of Gliomastix murorum", World J. Microbiol. Biotechnol, 25, pp.829~833 (2009)
- Khan SA, "A new strain of Arthrinium phaeospermum isolated from Carex kobomugi Ohwi is capable of gibberellin production", Biotechnol. Lett, 31, pp.283~287 (2009)
- Khan SA, "Plant growth promotion and Penicillium citrinum", BMC Microbiol, 8, pp.231~ (2008)
- 김봉수, "Remarkable Bacterial Diversity in the Tidal Flat Sediment as Revealed by16S rDNA Analysis", Journal of Microbiology and Biotechnology, 14(1), pp.205~211 (2004), 한국미생물·생명공학회
- 길이종, "보은 지역의 소나무(Pinus densiflora)에서 분리한 내생균의 동정과 다양성", 한국균학회지, 37(2), pp.130~133 (2009), 한국균학회
- Lambshead PJD, "Detection of differences among assemblages of marine benthic species based on anssessment of dominance and diversity", J. Nat. Hist, 17, pp.859~874 (1983)
- 임동옥, "태안해안국립공원 일대의 특정식물과 귀화식물", 한국환경생태학회지, 24(2), pp.117~129 (2010), 한국환경생태학회
- Margalef R., "Information theory in ecology", Gen. Syst, 3, pp.36~71 (1958)
- Opelt K, "Diversity and antagonistic potential of bacteria associated with bryophytes from nutrient poor habitats of baltic sea coast", Appl. Microbiol, 70, pp.6569~6579 (2004)
- Pielou EC, "Ecological diversity", pp.165~ (1975), John Wiley
- Simpson EH, "Measurement of diversity", Nature, 163, pp.688~ (1949)
- Tao G, "Whole rDNA analysis reveals novel and endophytic fungi in Bletilla ochracea (Orchidaceae)", Fungal. Divers, 33, pp.101~122 (2008)
- Tamura K, "MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods", Mol. Biol. Evol, 28, pp.2731~2739 (2011)
- Vazquez MM, "Interaction between arbuscular mycorrhizal fungi and other microbial inoculants (Azospirillum, Pseudomonas, Trichoderma) and their effects on microbial population and enzyme activities in the rhizosphere of maize plants", Appl. Soil. Ecol, 15, pp.261~272 (2000)
- Waller F, "The endophytic fungus Piriformospora indica reprograms barley to saltstress tolerance, disease resistance, and higher yield", PNAS, 102, pp.13386~13391 (2005)
- Williams AT, "Integrated management methods monitoring environmental changes in coastal dune ecosystem", Porc. 3rd European Marine Science and Technology Conference, Brusells, European Commission 2, pp.642~653 (1998)
- Whittaker RH., "Evolution of species diversity in land communities", Evol. Biol, 10, pp.1~67 (1977)
- Yamada A, "Isolation of Tricholoma matsutake and T. bakamatsutake cultures from field-collected ectomycorrhizas", Mycoscience, 42, pp.43~50 (2001)
- 유영현, "독도의 자생식물 뿌리에서 분리한 내생진균의 다양성과 생장촉진활성", 생명과학회지, 21(7), pp.992~996 (2011), 한국생명과학회
- You YH, "Cadophora malorum Cs-8-1 as a new fungal strain producing gibberellins isolated from Calystegia soldanella", J. Basic Microbiol, 52, pp.1~5 (2012)