브로콜리 꽃송이 및 줄기의 항산화, 항균 및 대장암 세포 생육억제효과
Antioxidative, Antimicrobial, and Anti-proliferative Activities of the Floret and Stalk of Broccoli (Brassica oleracea L.)
1
조회
0
다운로드
10
인용
0
참고문헌
키워드
초록
In the course of study for a use for non-edible parts of broccoli (Brassica oleracea L), and the development of processed foodutilizing these parts, edible floret and non-edible stalk were extracted with ethanol and different organic solvent fractions wereprepared. With 10 different extracts and fractions, their useful components and various biological activities, such as antioxidant,antimicrobial and anti-proliferation activity, were investigated. The stalk has more abundant water soluble carbohydrate whencompared with the floret, and floret has higher hexane-soluble pigments. Analysis of total flavonoid and total polyphenol contentsshowed that the floret has 1.5~1.99 times higher concentrations than the stalk. Among the fractions, ethylacetate (EA)fractions have the highest amount of total flavonoid and total polyphenol. The stalk and floret possessed 9.45 and 42.01 mgtotalflavonoid/g, respectively. In the antioxidation activity assay, the EA fraction of floret showed strong radical scavengingactivity and reducing power, while the n-hexane fraction of the stalk exhibited nitrite scavenging activity. In the antimicrobialactivity assay, the EA fraction of floret showed a strong and broad-range of antibacterial activity, irrespective of gram positive orgram negative bacteria. In a while, the hexane and EA fractions revealed anti-proliferative effects against the human colorectalcancer cell HCT-116. Strong anti-proliferative activities were found in the hexane fraction of stalk (18.4% of cell viability), andthe n-butanol fraction of floret (6.9% of cell viability). Our results suggest that the further study of the characterization of activefractions and the identification of active components from different parts of broccoli are needed to develop functional foods ornovel plant-derived medicines.
Abstract
In the course of study for a use for non-edible parts of broccoli (Brassica oleracea L), and the development of processed foodutilizing these parts, edible floret and non-edible stalk were extracted with ethanol and different organic solvent fractions wereprepared. With 10 different extracts and fractions, their useful components and various biological activities, such as antioxidant,antimicrobial and anti-proliferation activity, were investigated. The stalk has more abundant water soluble carbohydrate whencompared with the floret, and floret has higher hexane-soluble pigments. Analysis of total flavonoid and total polyphenol contentsshowed that the floret has 1.5~1.99 times higher concentrations than the stalk. Among the fractions, ethylacetate (EA)fractions have the highest amount of total flavonoid and total polyphenol. The stalk and floret possessed 9.45 and 42.01 mgtotalflavonoid/g, respectively. In the antioxidation activity assay, the EA fraction of floret showed strong radical scavengingactivity and reducing power, while the n-hexane fraction of the stalk exhibited nitrite scavenging activity. In the antimicrobialactivity assay, the EA fraction of floret showed a strong and broad-range of antibacterial activity, irrespective of gram positive orgram negative bacteria. In a while, the hexane and EA fractions revealed anti-proliferative effects against the human colorectalcancer cell HCT-116. Strong anti-proliferative activities were found in the hexane fraction of stalk (18.4% of cell viability), andthe n-butanol fraction of floret (6.9% of cell viability). Our results suggest that the further study of the characterization of activefractions and the identification of active components from different parts of broccoli are needed to develop functional foods ornovel plant-derived medicines.
참고문헌 (28)
- 안선미, "체리(Prunus avium L.)의 항균 및 항산화 활성", 한국미생물·생명공학회지, 37(4), pp.371~376 (2009), 한국미생물·생명공학회
- 안선미, "해조류 추출물의 항산화 및 nitrite 소거활성 평가", 생명과학회지, 21(4), pp.576~583 (2011), 한국생명과학회
- 최영현, "HepG2 인체간암세포의 세포주기조절인자 발현에 미치는 sulforaphane의 영향", 생명과학회지, 16(7), pp.1235~1242 (2006), 한국생명과학회
- 조경련, "브로콜리 분말을 첨가한 설기떡의 품질 특성", 한국식품영양학회지, 22(2), pp.229~237 (2009), 한국식품영양학회
- Jayakumar T, "A novel antithrombotic effect of sulforaphane via activation of platelet adenylate cyclase: ex vivo and in vivo studies", J. Nutr. Biochem, 24, pp.1086~1095 (2013)
- 정병걸, "브로콜리 유래 sulforaphane에 의한 NAG-1과 p21 유전자의 발현 조절", 생명과학회지, 22(3), pp.360~365 (2012), 한국생명과학회[DOI]
- 장민우, "추출용매에 따른 브로콜리의 항산화와 항염증에 대한 효과", 한국식품위생안전성학회지, 27(4), pp.461~465 (2012), 한국식품위생안전성학회
- 김지인, "마(Dioscorea batatas Decne)의 항균, 항산화 및 항혈전 활성 평가", 한국미생물·생명공학회지, 37(2), pp.133~139 (2009), 한국미생물·생명공학회
- 김지영, "조리된 브로콜리의 항산화 효과 및 Sulforaphane 함량 분석", 동아시아식생활학회지, 19(4), pp.564~569 (2009), 동아시아식생활학회
- Kim MR, "Effect of processing on the content of sulforaphane of broccoli", Korean J. Soc. Food Sci, 13, pp.422~426 (1997)
- Kim SM, "The antioxidant ability and nitrite scavenging ability of plant extracts", Korean J. Food Sci. Technol, 33, pp.626~632 (2001)
- 손호용, "재배마 (Dioscorea batatas Decne)의 주아로부터 분리된 phenanthrene 유도체의 항진균 활성", 생명과학회지, 16(4), pp.647~652 (2006), 한국생명과학회
- 박양원, "고온에서의 브로콜리 부위별 추출물의 항산화성 및 항균성", 한국식품영양과학회지, 34(6), pp.759~764 (2005), 한국식품영양과학회
- 이준구, "국내 브로콜리( Brassica oleracea L. var. italica) 유전자원 내 Glucosinolate 함량 변이", 원예과학기술지, 30(6), pp.743~750 (2012), 한국원예학회[DOI]
- 이재준, "브로콜리 싹 에탄올 추출물이 고지방식이를 급여한 흰쥐의 콜레스테롤 저하 및 비만 억제효과에 미치는 영향", 한국식품영양과학회지, 38(3), pp.309~318 (2009), 한국식품영양과학회
- 이재준, "브로콜리 싹의 이화학적 성분", 생명과학회지, 19(2), pp.192~197 (2009), 한국생명과학회
- 이성진, "Sodium selenate의 토양관주 처리방법에 따른 서양채소의 생육과 무기성분, ascorbic acid, nitrate 및 세레늄 함량에 미치는 영향", 시설원예‧식물공장, 17(1), pp.43~50 (2008), 한국생물환경조절학회
- 이원길, "Sulforaphane에 의한 p53 및 caspase-3 의존 신호전달계를 통한 인간 연골암 세포주 HTB-94에서의 세포사멸 기전 연구", 생명과학회지, 21(6), pp.851~857 (2011), 한국생명과학회
- Mi L, "The role of protein binding in induction of apoptosis by phenethyl isothiocyanate and sulforaphane in human non-small lung cancer cells", Cancer Res, 67, pp.6409~6416 (2007)
- Moon DO, "Sulforaphane suppresses TNF-alpha-mediated activation of NFkB and induces apoptosis through activation of reactive oxygen species-dependent caspase-3", Cancer Lett, 274, pp.132~142 (2009)
- 오재복, "브로콜리 줄기 분말을 혼합한 파운드 케익의 항산화 활성과 품질 특성에 미치는 영향", 한국식품영양학회지, 24(4), pp.567~576 (2011), 한국식품영양학회
- 오유성, "브로콜리잎 분말 첨가 재래식 고추장의 이화학적 및 기능적 특성", 한국식품영양과학회지, 42(5), pp.675~681 (2013), 한국식품영양과학회
- Jun Ho Park, "Sulforaphane inhibits the Th2 immune response in ovalbumin-induced asthma", BMB Reports, 45(5), pp.311~316 (2012), 생화학분자생물학회[DOI]
- 박성영, "Sulforaphane에 의한 HeLa 인체자궁경부암세포의 증식 억제 기전 연구", 생명과학회지, 15(3), pp.397~405 (2005), 한국생명과학회
- 석대은, "브로콜리 용매추출물의 Bioactive Organosulfur 화합물의 분리 및 동정", 한국식품영양과학회지, 32(3), pp.315~319 (2003), 한국식품영양과학회
- 손호용, "장수상황버섯 균사체를 이용한 고체 발효한약재의 대장암 세포성장 억제 활성", 생명과학회지, 20(8), pp.1268~1275 (2010), 한국생명과학회
- Valentina U, "Sugars, organic acids, phenolic composition and antioxidant activity of sweet cherry (Prunus avium L.)", Food Chem, 107, pp.185~192 (2007)
- 유선미, "인간 유방암세포 MDA-MB-231에서 Sulforaphane (SFN)과 Sodium Nitroprusside (SNP)에 의한 C-Jun N-terminal Kinase (JNK) 경로를 통한 증식 억제 및 세포사멸 유도", 대한암예방학회지, 16(4), pp.318~325 (2011), 대한암예방학회