순천만에 자생하는 염생식물 근권에서 유래한 해양세균의 계통학적 분석 및 다양성
Phylogenetic Analysis and Diversity of Marine Bacteria Isolated from Rhizosphere Soils of Halophyte in Suncheon Bay
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초록
순천만의 염생식물 근권에서 정주하는 세균의 분리를 위해 순천만에서 우점하는 자생식물인 칠면초 군락 3개 지점을 선발하여 샘플링 하였다. 시료는 marine broth, tryptic soy broth 한천배지를 통해 분리되었으며, 형태학적인 구분을 통해 순수분리되었다. 분리주의 16S rDNA를 분석하여총 92 균주가 동정되었다. 이들의 유연관계 확인을 위한 계통수 작성 결과, 각각 firmicutes (56.5%), gamma-proteobacteria (29.3%), alpha-proteobacteria (5.4%), actinobacteria (5.4%), bacteroidetes (3.3%)에 속하였다. Shannon’s Diversity index (H')를 산출하였을 때 각각 1.675, 1.924, 2.04로, 채취 지점별로 종 다양성의 차이를 보였다.
Abstract
The bacterial diversity of the rhizosphere soil of S. japonica native to Suncheon bay was analyzed. Ninety two strains showing different morphological characteristics were isolated from the soils around the community of S. japonica. Bacterial diversity and distributions were studied by phylogenetic analysis of the partial 16S rDNA sequences. Ninety two strains were partially sequenced and analyzed phylogenetically. These strains were composed of 5 phyla firmicutes (56.5%), gamma-proteobacteria (29.3%), alpha-proteobacteria (5.4%), actinobacteria (5.4%), bacteroidetes (3.3%) and Shannon’s diversity index (H') were different from each of sampling sites (1.675, 1.924 and 2.04). Eleven isolates were presumed to be novel species candidates based on similarity analysis of the 16s rRNA gene sequences. Overall, Firmicutes and gamma-proteobacteria of the rhizosphere soil of S. japonica showed a high diversity.
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