Bacterial Diversity in the Rhizosphere of Halophyte Phragmites communis at the Western Coastal Mudflats of Korea
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This study investigated the population densities and diversity of heterotrophic bacteria, and the rhizosphere-to-soil ratios (R/S) in the rhizosphere soil of halophyte Phragmites communis at the western coastal mudflats of Korea. The population densities of aerobic heterotrophic bacteria on the rhizosphere soil of P. communis were in the range of 3.3 ± 0.9 × 107~1.2 ± 0.5 × 108 cfu g-1 dry weight (d. wt.). Population densities of amylolytic bacteria ranged from 1.1 ± 0.2 × 106 to 3.0 ± 1.2 × 106 cfu g-1 d. wt., while those of cellulolytic bacteria and proteolytic bacteria ranged from 5.6 ± 2.3 × 106 to 1.5 ± 0.3 × 107 cfu g-1 d. wt. and from 1.4 ± 0.3 × 106 to 3.5 ± 2.3 × 107 cfu g-1 d. wt., respectively. The R/S ratios ranged from 2.26 to 6.89. Genetic (16S DNA) analysis of fifty-one isolates from the roots of P. communis suggested that the dominant species were closely related to the γ-proteobacteria group (18 clones) and the α-proteobacteria group (14 clones). We found that halophyte species and mudflat environment both affected the rhizosphere bacterial communities.
Abstract
This study investigated the population densities and diversity of heterotrophic bacteria, and the rhizosphere-to-soil ratios (R/S) in the rhizosphere soil of halophyte Phragmites communis at the western coastal mudflats of Korea. The population densities of aerobic heterotrophic bacteria on the rhizosphere soil of P. communis were in the range of 3.3 ± 0.9 × 107~1.2 ± 0.5 × 108 cfu g-1 dry weight (d. wt.). Population densities of amylolytic bacteria ranged from 1.1 ± 0.2 × 106 to 3.0 ± 1.2 × 106 cfu g-1 d. wt., while those of cellulolytic bacteria and proteolytic bacteria ranged from 5.6 ± 2.3 × 106 to 1.5 ± 0.3 × 107 cfu g-1 d. wt. and from 1.4 ± 0.3 × 106 to 3.5 ± 2.3 × 107 cfu g-1 d. wt., respectively. The R/S ratios ranged from 2.26 to 6.89. Genetic (16S DNA) analysis of fifty-one isolates from the roots of P. communis suggested that the dominant species were closely related to the γ-proteobacteria group (18 clones) and the α-proteobacteria group (14 clones). We found that halophyte species and mudflat environment both affected the rhizosphere bacterial communities.
참고문헌 (37)
- Altschul SF, "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res, 5, pp.3389~3402 (1997)
- Assigbetse K, "Soil bacterial diversity response to root colonization by an ectomycorrhizal fungus are not root-growth-dependent", Microb Eco, 50, pp.350~359 (2005)
- Atlas RM, "Microbial Ecology: Fundamentals and Application", pp.1992, Benjamin
- Benoit JM, "Geochemical and biological controls over methylmercury production and degradation in aquatic ecosystems", ACS Symp Ser, 835, pp.262~297 (2003)
- Brimecombe MJ, "The effect of root exudates on rhizosphere microbial communities. In: The Rhizosphere: Biochemistry and Organic Substances at Soil-Plant Interface", pp.95~140 (2001), Marcel Dekker
- Choi GK, "Interaction between saprophytic bacterial distribution and their extracellular enzyme activities in the sediment of the Yellow sea near Seocheon", The Microorganisms and Industry, 22, pp.119~126 (1996)
- Degens BP, "Decreases in organic C reserves in soils can reduce the catabolic diversity of soil microbial communities", Soil Biol Biochem, 32, pp.189~196 (2000)
- Duineveld BM, "Analysis of the dynamics of bacterial communities in the rhizosphere of the chrysanthemum via denaturing gradient gel electrophoresis and substrate utilization patterns", Appl Environ Microbiol, 64, pp.4950~4957 (1998)
- Duineveld BM, "Analysis of bacterial communities in the rhizosphere of chrysanthemum via denaturating gradient gel electrophoresis of PCR- amplified 16S rRNA as well as DNA fragments coding for 16S rRNA", Appl Environ Microbiol, 67, pp.172~178 (2001)
- Gelsomino A, "Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis", J Microbiol Methods, 38, pp.1~15 (1999)
- Grant SB, "Generation of enterococci bacteria in a coastal saltwater marsh and impact on surf zone water quality", Environ Sci Technol, 35, pp.2407~2416 (2001)
- Gray JP, "Phylogenetic analysis of the bacterial communities in marine sediments", Appl Environ Microbiol, 62, pp.4049~4059 (1996)
- Gray TRG, "The Ecology of Soil Bacteria 681" (1968), University of Toronto Press
- Grayston SJ, "Rhizosphere carbon flow in tree, in comparison with annual plants: the importance of root exudation and its impact on microbial activity and nutrient availability", Appl Soil Ecol, 5, pp.29~56 (1996)
- Grayston SJ, "Selective influence of plant species on microbial diversity in the rhizosphere", Soil Biol Biochem, 30, pp.369~378 (1998)
- Holding AJ, "Routine biochemical tests. In: Methods in Microbiology", pp.1~32 (1971), Academic Press Inc Ltd
- Howes BL, "Interception of freshwater and nitrogen transport from uplands to coastal waters: the role of salt marshes. In: Estuarine shores: evolution, environments and human alteration", pp.287~310 (1996), John Wiley & Sons
- KIM, BONG-SOO, "Remarkable Bacterial Diversity in the Tidal Flat Sediment as Revealed by16S rDNA Analysis", Journal of Microbiology and Biotechnology, 14(1), pp.205~211 (2004), 한국미생물·생명공학회
- Bong-Soo Kim, "Archaeal Diversity in Tidal Flat Sedimentas Revealed by 16S rDNA Analysis", The Journal of Microbiology, 43(2), pp.144~151 (2005), 한국미생물학회
- Kim SJ, "Distribution of heterotrophic bacteria and extracellular enzyme activities in the sediment of South Sea, Korea", Kor J Microbiol, 30, pp.383~390 (1992)
- Latour X, "The composition of fluorescent pseudomonad populations associated with roots is influenced by plant and soil type", Appl Environ Microbiol, 62, pp.2449~2456 (1996)
- Lee GH, "Distribution of aerobic", anaerobic saprophytic bacteria in the sediments of the Yellow Sea near Kunsan, Korea, 1996, pp.48, 227~232
- Lee MS, "Bacterial diversity in the mud flat of Suncheon bay, Chunnam Province, by 16SrRNA gene analysis", Kor J Microbiol, 37, pp.137~144 (2001)
- Maloney PE, "Bacterial community structure in relation to the carbon environments in lettuce and tomatorhizospheres and in bulk soils", Microb Eco, 34, pp.109~117 (1997)
- Marshner P, "Soil and plant specific effects on bacterial community composition in the rhizosphere", Soil Biol Biochem, 33, pp.1437~1445 (2001)
- Metting BF, "Soil Microbial ecology" (1993), Marcel Dekker
- Neal JL, "Changes in the rhizosphere microflora of spring wheat induced by disomic substitution of a chromosome", Can J Microbiol, 16, pp.153~158 (1970)
- Neal JL, "Changes in rhizosphere populations of selected groups of physiological groups of bacteria related to substitution of specific pairs of chromosomes in spring wheat", Plant Soil, 39, pp.209~212 (1970)
- Park SH, "Taxonomical study of heterotrophic bacteria in the rhizosphere of halophytes in western and southern coastal area of Korea" (2004), Kunsan National University
- Park SH, "Bacterial diversity in the rhizosphere of halophyte Suaeda japonica in western and Southern mudflats of Korea", J Ecol Biol, 29, pp.399~404 (2006)
- Paul EA, "Soil microbiology and biochemistry" (1988), Academic Press
- Saitou N, "The neighbor-joining method: a new method for reconstruction phylogenetic trees", Mol Biol Evol, 4, pp.406~425 (1987)
- Singh G, "Root exudates as determinant of rhizospheric microbial biodiversity. In: Microbial Activity in the Rhizosphere", pp.39~53 (2006), Springer-Verlag
- Smith SV, "Coastal metabolism and the oceanic organic carbon balance", Rev Geophys, 31, pp.75~89 (1993)
- Teal JM, "Salt marsh values; retrospection from the end of the century. In: Concepts and Controversies in Tidal Marsh Ecology", pp.3~7 (2000), Kluwer Academic Publishers
- Woldendorp JW, "The rhizoshere as part of the plant-soil system. In Structure and Functioning of Plant Population. Verhandeligen der Koninklijke, Nederlandse Akademie van Wetsenschappen, Afdeling Natuukunde, Twede Reeks, deel 70", ~ (1978)
- Wollum AG, "Cultural methods for soil microorganisms. In Method of Soil Analysis, Part 2: Chemical and Microbiological Properties", pp.781~802 (1982), Madison