제주 연안에서 분리된 해양방선균의 이화학적 특성 및 다양성
Physico-chemical Characteristics and Diversity of Marine Actinomycetes Isolated from the Coast of Jeju Island
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제주도 연안해역 4개 지역(한림, 애월, 신촌, 함덕) 해수의 온도, 염분농도, 용존산소량(DO), 화학적 산소요구량(COD), 부유물질(SS), 암모니아성 질소(NH3-N), 질산성 질소(NO3-N) 및 아질산성 질소(NO2-N)와 같이 다양한 이화학적 특성을 확인하였다. 해수의 평균 온도는26.23~28.6˚C, 염분농도는 31.4~32.88‰, pH는 8.15~8.35, COD는 0.48~0.91 mg L-1, DO는 6.78~6.87 mg L-1로 나타났다. 해수에서 분리된 해양방선균은 총 52종으로 제주시동부지역(A, B)에서는 24균주, 서부지역(C, D)은 28균주가 분리되었다. 분리된 해양방선균은 16S rRNA 염기서열 분석을 이용하여 최종적으로 동정되었으며, 염기서열정보를 기초로하여 유사도(similarity)를 조사하였다. 분리된 방선균의 16S rRNA 염기서열 분석을 통하여계통 분류학적으로 어떤 division에 속하는지를 확인하여 본 결과 제주도 제주시 동부지역(Site A, B) 해양에서분리된 방선균 24균주는 Gram positive bacteria (division)/Actinobacteria (class)/Actinomycetales (order)/Streptomy-
Abstract
To investigate the variations of physico-chemical factors in four stations (Hanlim,Aewol, Sinchon, Hamdeok) at Jeju coastal area, Water temperature, Salinity, dissolved oxygen (DO), pH, chemical oxygen demand (COD), suspended solid (SS), ammonia-nitrogen (NH4-N),nitrite-nitrogen (NO3-N), nitrate-nitrogen (NO2-N) were analysed. The ranges of water temperature were from 26.23 to 28.6˚C, the salinity were from 31.4 to 32.88‰, the pH were from 8.15 to 8.35, the chemical oxygen were from 0.48 to 0.91 mg L-1. A total of 52 strains of marine actinomycetes was isolated from Jeju Island coastal area. They were characterized by determining morphological and physio-biochemical properties, the API kit and confirmed by molecular methods including partial sequencing of 16S rRNA. A neighbor-joining tree of partial 16S rRNA sequences divided the 52 isolates in 2 major groups, 22 strains of Gram positive bacteria/Actinobacteria (division)/Actinomycetales (order)/Streptomycineae (suborder)/Streptomycetaceae (family)/Streptomyces (93.1%) and 2 strains of Streptospotangineae (suborder)/Nocardiopsaceae (family)/Nocardiopsis (6.9%).
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