동시당화발효공정을 위한 바이오캡슐 형성
Bio-capsule Formation for Synchronous Saccharification and Fermentation Process
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본 연구에서는 동시당화발효공정으로 바이오에탄올을 생산하기 위하여 바이오캡슐 형성을 시도하였다. 다수의 당화곰팡이 균주들과 발효 효모 균주들이 먼저 탐색되었다. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201 및 P. chrysogenum KACC 44363이 α-amylase와 glucoamylase 와 같은 당화 효소를 우수하게 생산하는 균주이었으며, Saccharomyces cerevisiae IFO-M-07이 조사된 균주 중에서가장 에탄올 생산능이 높았다. 다음으로 pellet 형성 및 바이오 캡슐 형성을 위한 최적 조건을 평가하였다. 모든 조사된곰팡이 모두 pellet을 형성하였으며, 바이오캡슐의 최적조건은 28℃, 120 rpm이었다. 최종적으로 형성된 바이오캡슐을 이용하여 동시당화발효를 수행하여, Aspergillus sp. BCNU 6200의 바이오캡슐(Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07)이 10일간 발효시 30℃, 120 rpm에서3.9%의 에탄올을 생산함을 확인하였다. 본 실험 결과는 동시당화발효 공정으로 바이오에탄올을 생산하는데 있어서 바이오캡슐을 활용함에 관한 유용한 정보를 제공하고 있다.
Abstract
For the production of bioethanol by the synchronous saccharification and fermentation (SSF) process, biocapsule formation was attempted. Many saccharifying fungal strains and fermentative yeast strains were first screened. Aspergillus sp. BCNU 6200, Penicillium sp. BCNU 6201, and P. chrysogenum KACC 44363 were found to be excellent producers of saccharifying enzymes such as α-amylase and glucoamylase. Saccharomyces cerevisiae IFO-M-07 showed the highest ethanol productivity among the tested strains. Secondly, we determined the optimal conditions for pellet formation, and those for bio-capsule formation. All the tested fungal strains formed pellets, and the optimal conditions for bio-capsule formation were 28℃ and 120 rpm. Lastly, SSF process was performed using a bio-capsule. An ethanol yield of 3.9% was achieved by using the Aspergillus sp. BCNU 6200 bio-capsule (Aspergillus sp. BCNU 6200 + S. cerevisiae IFO-M-07) at 30℃ with shaking at 120 rpm during the 10 days of incubation. The results provide useful information on the application of a bio-capsule in bioethanol production under the SSF process.
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