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단수수 착즙액으로부터 에탄올 생산을 위한 반응표면분석법을 이용한 효모 발효조건 최적화

Optimization of Fermentation Conditions for the Ethanol Production from Sweet Sorghum Juice by Saccharomyces cerevisiae using Response Surface Methodolgy

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초록

Optimization of initial total sugar concentration of sweet sorghum juice, aeration time and aeration rate on ethanol production was performed by response surface methodology (RSM). The optimum conditions for ethanol production from concentrated sweet sorghum juice were determined as follows: initial total sugar concentration, 21.2 Brix; aeration time, 7.66h;aeration rate, 1.22 vvm. At the optimum conditions, the maximum ethanol yield was predicted to be 91.65% by model prediction. Similarly, 92.98% of ethanol yield was obtained by verification experiment using optimum conditions after 48 h of fermentation. This result was in agreement with the model prediction.

Abstract

Optimization of initial total sugar concentration of sweet sorghum juice, aeration time and aeration rate on ethanol production was performed by response surface methodology (RSM). The optimum conditions for ethanol production from concentrated sweet sorghum juice were determined as follows: initial total sugar concentration, 21.2 Brix; aeration time, 7.66h;aeration rate, 1.22 vvm. At the optimum conditions, the maximum ethanol yield was predicted to be 91.65% by model prediction. Similarly, 92.98% of ethanol yield was obtained by verification experiment using optimum conditions after 48 h of fermentation. This result was in agreement with the model prediction.

참고문헌 (11)

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  2. ghou, "Refining bioethanol from stalk juice of sweet sorghum by immobilized yeast fermentation", Renewable Energy, 33, pp.1130~1135 (2008)
  3. Billa Eilla, "Structure and compositoin of sweet sorghum stalk components", Industrial Crops & Productions, 6, pp.297~302 (1997)
  4. Jianliang Yu, "Ethanol production by solid state fermentation of sweet sorghum using thermotolerant strain", Fuel Processing Technology, 89, pp.1056~1059 (2008)
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  6. S. Alfenore, "Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process", Appl. Microbiol Biotechnol, 63, pp.537~542 (2003)
  7. Ezhumalai Sasikumar, "Optimization of Process Conditions Using Response Surface Methodology(RSM) for Ethanol Production from Pretreated Sugarcane Bagasse: Kinetic and Modeling", Bioenergy Research, 1, pp.239~247 (2008)
  8. Ramesh Balusu, "Optimization of critical medium components using response surface methodology for ethanol production from cellulosic bioimass by Clostridium thermocellum SS19", Process Biochemistry, 40(9), pp.3025~3030 (2005)
  9. hilkumar SR, "Optimization of medium composition for alkai-stable xylanase production by Aspergillus fischeri Fxn 1 in solid-state fermentation using central composite rotary design", Bioresource Technology, 96, pp.1380~1386 (2005)
  10. Altaf MD, "Single step fermentation of starch to L(+) lactic acid by Lactobacillus amylophilus GV6 in SSF using inexpensive nitrogen source to relace peptoneand yeast extract optimization by RSM", Biochemistry, 41, pp.465~472 (2006)
  11. Petros Katapodis, "Opitimization of xylanase production by Chaetomium thermophilum in wheat straw using response surface methodology", Biochemical Engineering Journal, 35, pp.136~141 (2007)

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