Cultivable Microbial Diversity in Domestic Bentonites and Their Hydrolytic Enzyme Production
0
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
We have isolated and identified 72 bacterial strains from four bentonite samples collected at the mining areas located in Gyeongsangbuk-do, Republic of Korea, and measured their hydrolytic enzyme (α-amylase, protease, and cellulase) activities to identify the isolates with industrial-use potential. Most of the isolates belonged to the Bacillaceae, with minor portions being from the Paenibacillaceae, Micrococcaceae, and Bacillales Family XII at the family level. Of the strains isolated, 33 had extracellular α-amylase activity, 30 strains produced cellulase, and 35 strains produced protease. Strain MBLB1268, having the highest α- amylase activity, was identified as Bacillus siamensis (0.38 ± 0.06 U/ml). Bacillus tequilensis MBLB1223, isolated from Byi33-b, showed the highest cellulase activity (0.26 ± 0.04 U/ml), whereas Bacillus wiedmannii MBLB1197, isolated from Zdb130-b, exhibited the highest protease activity (54.99 ± 0.78 U/ml). These findings show that diverse bacteria of the Bacillaceae family adhere to and exist in bentonite and are potential sources of industrially useful hydrolytic enzymes.
Abstract
We have isolated and identified 72 bacterial strains from four bentonite samples collected at the mining areas located in Gyeongsangbuk-do, Republic of Korea, and measured their hydrolytic enzyme (α-amylase, protease, and cellulase) activities to identify the isolates with industrial-use potential. Most of the isolates belonged to the Bacillaceae, with minor portions being from the Paenibacillaceae, Micrococcaceae, and Bacillales Family XII at the family level. Of the strains isolated, 33 had extracellular α-amylase activity, 30 strains produced cellulase, and 35 strains produced protease. Strain MBLB1268, having the highest α- amylase activity, was identified as Bacillus siamensis (0.38 ± 0.06 U/ml). Bacillus tequilensis MBLB1223, isolated from Byi33-b, showed the highest cellulase activity (0.26 ± 0.04 U/ml), whereas Bacillus wiedmannii MBLB1197, isolated from Zdb130-b, exhibited the highest protease activity (54.99 ± 0.78 U/ml). These findings show that diverse bacteria of the Bacillaceae family adhere to and exist in bentonite and are potential sources of industrially useful hydrolytic enzymes.
참고문헌 (29)
- Bergaya F, "General introduction: clays, clay minerals, and clay science", Develop. Clay Sci, 1, pp.1~18 (2006)
- Seppälä A, "Effect of layer charge on the crystalline swelling of Na+, K+ and Ca2+ montmorillonites: DFT and molecular dynamics studies", Clay Miner, 51, pp.197~211 (2016)
- González-Pradas E, "Influence of the physical-chemistry properties of an acidactivated bentonite in the bleaching of olive oil", J. Chem. Technol. Biotechnol, 57, pp.213~216 (1993)
- Allo WA, "Mineralogy, chemistry and potential applications of a white bentonite in San Juan province, Argentina", Appl. Clay Sci, 25, pp.237~243 (2004)
- Bereket G, "Removal of Pb(II), Cd(II), Cu(II), and Zn(II) from aqueous solutions by adsorption on bentonite", J. Colloid Interf. Sci, 187, pp.338~343 (1997)
- Oya A, "Antimicrobial and antifungal agents derived from clay minerals", J. Mater. Sci, 29, pp.11~14 (1994)
- Dai Q, "Interaction between bentonite and Bacillus litoralis strain SWU9", Appl. Clay Sci, 100, pp.88~94 (2014)
- Kang J, "Impact of clay minerals on bacterial diversity during the fermentation process of kimchi", Appl. Clay Sci, 154, pp.64~72 (2018)
- Seo SM, "A simple synthesis of nitrate cancrinite from natural bentonite", J. Porous Mat, 25, pp.1561~1565 (2018)
- 노성운, "Arthrobacter soli sp. nov., a Novel Bacterium Isolated from Wastewater Reservoir Sediment", The Journal of Microbiology, 46(1), pp.40~44 (2008), 한국미생물학회
- Pruesse E, "SINA: accurate highthroughput multiple sequence alignment of ribosomal RNA genes", Bioinformatics, 28, pp.1823~1829 (2012)
- Yoon SH, "Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies", Int. J. Syst. Evol. Microbiol, 67, pp.1613~1617 (2017)
- Kimura M, "A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences", J. Mol. Evol, 16, pp.111~120 (1980)
- Kumar S, "MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets", Mol. Biol. Evol, 33, pp.1870~1874 (2016)
- Saitou N, "The neighbour-joining method: a new method for reconstructing phylogenetic trees", Mol. Biol. Evol, 4, pp.406~425 (1987)
- Miller RA, "Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments", Int. J. Syst. Evol. Microbiol, 66, pp.4744~4753 (2016)
- Miller RA, "Intra clade variability in toxin production and cytotoxicity of Bacillus cereus group type strains and dairy-associated isolates", Appl. Environ. Microbiol, 84, pp.2479~2517 (2018)
- Kantas D, "A feed additive containing Bacillus toyonensis (Toyocerin(®)) protects against enteric pathogens in postweaning piglets", J. Appl. Microbiol, 118, pp.727~738 (2015)
- Kim M, "Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes", Int. J. Syst. Evol. Microbiol, 64, pp.346~351 (2014)
- Moreno ML, "Analysis and characterization of cultivable extremophilic hydrolytic bacterial community in heavy-metal-contaminated soils from the Atacama Desert and their biotechnological potentials", J. Appl. Microbiol, 113, pp.550~559 (2012)
- Hendricks CW, "New solid medium for enumerating cellulose-utilizing bacteria in soil", Appl. Environ. Microbiol, 61, pp.2016~2019 (1995)
- Anson ML, "The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin", J. Gen. Physiol, 22, pp.79~89 (1938)
- Das K, "Purification and biochemical characterization of a thermostable, alkaliphilic, extracellular α- amylase from Bacillus subtilis DM-03, a strain isolated from the traditional fermented food of India", Biotechnol. Appl. Biochem, 40, pp.291~298 (2004)
- Hye Hee Jeon, "Screening and Characterization of Potential Bacillus Starter Cultures for Fermenting Low-Salt Soybean Paste (Doenjang)", Journal of Microbiology and Biotechnology, 26(4), pp.666~674 (2016), 한국미생물·생명공학회[DOI]
- Hill J, "Environmental, economic, and energetic costs and benefits of biodiesel and ethanol biofuels", Proc. Natl. Acad. Sci. USA, 314, pp.1598~1600 (2006)
- Ram L, "Screening isolation and characterization of cellulase producing micro-organisms from soil", Int. J. Pharm. Sci. Inv, 3, pp.12~18 (2014)
- Soni SK, "Production of xylanase-cellulase complex by Bacillus subtilis NS7 for the biodegradation of agro waste residues", Lignocellulose J, 1, pp.196~209 (2014)
- Sumantha A, "Microbiology and industrial biotechnology of food-grade proteases: a perspective", Food Technol. Biotechnol, 44, pp.211~220 (2006)
- Ouattara HG, "Regulation of the synthesis of pulp degrading enzymes in Bacillus isolated from cocoa fermentation", Food Microbiol, 63, pp.255~262 (2017)