Identification of a Newly Isolated Protease-producing Bacterium, Bacillus subtilis FBL-1, from Soil
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초록
A novel proteolytic bacterium was isolated from soil at Yeungnam University, South Korea. The strain, named FBL-1, was rod-shaped with a smooth surface. Biolog and API 50CHB test results revealed that strain FBL-1 was a Bacillus species. Based on 16S rDNA sequencing and chemotaxonomic characterization, the strain was identified as Bacillus subtilis because it had the highest homology with Bacillus subtilis subsp. subtilis NCIB 3610 (99.5%). In liquid culture at 37°C with shaking at 200 rpm, fructose and yeast extract were found to be the best carbon and nitrogen sources, respectively, for cell growth and protease production. The highest protease activity (451.640 U/ml) was obtained when the strain was cultured in medium containing 20 g/l of fructose and 5 g/l of yeast extract. Although further studies are needed to characterize the protease and enhance its activity, the newly isolated protein-degrading B. subtilis FBL-1 can be applicable for the production of peptides and for the degradation of proteins in various industries.
Abstract
A novel proteolytic bacterium was isolated from soil at Yeungnam University, South Korea. The strain, named FBL-1, was rod-shaped with a smooth surface. Biolog and API 50CHB test results revealed that strain FBL-1 was a Bacillus species. Based on 16S rDNA sequencing and chemotaxonomic characterization, the strain was identified as Bacillus subtilis because it had the highest homology with Bacillus subtilis subsp. subtilis NCIB 3610 (99.5%). In liquid culture at 37°C with shaking at 200 rpm, fructose and yeast extract were found to be the best carbon and nitrogen sources, respectively, for cell growth and protease production. The highest protease activity (451.640 U/ml) was obtained when the strain was cultured in medium containing 20 g/l of fructose and 5 g/l of yeast extract. Although further studies are needed to characterize the protease and enhance its activity, the newly isolated protein-degrading B. subtilis FBL-1 can be applicable for the production of peptides and for the degradation of proteins in various industries.
참고문헌 (18)
- Adinarayana K, "Purification and partial characterization of thermostable serine alkaline protease from a newly isolated Bacillus subtilis PE-11", AAPS Pharm. Sci. Tech, 4, pp.1~9 (2003)
- Anissa H, "Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone", J. Biosci. Bioeng, 110, pp.288~294 (2010)
- Anson ML, "The estimation of pepsin, trypsin, papain and cathepsin with hemoglobin", J. Gen. Physiol, 22, pp.79~89 (1938)
- Banerjee UC, "Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive", Process Biochem, 35, pp.213~219 (1999)
- Folin O, "On tyrosine and tryptophan determinations in proteins", J. Biol. Chem, 73, pp.627~650 (1927)
- Gupta R, "An overview on fermentation, downstream processing and properties of microbial alkaline proteases", Appl. Microbiol. Biotechnol, 60, pp.381~395 (2002)
- Horikoshi K, "Production of alkaline enzymes by alkalophilic microorganisms", Arg. Biol. Chem, 35, pp.1407~1414 (1973)
- Josephine FS, "Isolation, production and characterization of protease from Bacillus sp. isolated from soil sample", J. Microbiol. Biotechnol. Res, 2, pp.163~168 (2012)
- Kageyama K, "Studies on Aspergillus oryzae strain for sake brewing", J. Ferment. Technol, 33, pp.53~57 (1955)
- Kim OS, "Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species", Int. J. Syst. Evol. Microbiol, 62, pp.716~721 (2012)
- Masaaki Y, "Purification and properties of acid protease from Monascus sp. No. 3405", Agric. Biol. Chem, 48, pp.1637~1645 (1984)
- Nunokawa Y, "A study of the Koji protease", J. Soc. Brew, 53, pp.930~933 (1961)
- Oberoi R, "Characterization and wash performance analysis of a SDS-stable alkaline protease from Bacillus sp", World J. Microbiol. Biotechnol, 17, pp.493~497 (2001)
- Oguntoyinbo FA, "Phenotypic diversity and technological properties of Bacillus subtilis species isolated from okpehe, a traditional fermented condiment", World J. Microbiol. Biotechnol, 23, pp.401~410 (2007)
- 박형수, "Protease 생성균 Aeromonas hydrophila PB16의 분리 및 합성폐수처리능", 미생물학회지, 38(4), pp.235~240 (2002), 한국미생물학회
- Rao MB, "Molecular and biotechnological aspects of microbial proteases", Microbiol. Mol. Biol. Rev, 62, pp.597~635 (1998)
- Reddy LVA, "Purification and characterization of an organic solvent and detergent-tolerant novel protease produced by Bacillus sp. RKY3", J. Chem. Technol. Biotechnol, 83, pp.1526~1533 (2008)
- Reddy LVA, "Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3through Plackett-Burman and response surface methodological approaches", Bioresour. Technol, 99, pp.2242~2249 (2008)