부식토 유래 질산화세균 consortium의 질산화 유전자 거동 특성
Nitrifying-genes Dynamics in the Enriched Bacterial Consortium Inoculated with Humic Soil
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
In this study, the effects of ammonium concentration (117.5−1155.0 mg-N·l-1), nitrite concentration (0-50.0 mg-N·l-1), and temperature (15−35℃) on nitrification performance and its functional genes (amoA-arc, amoA-bac, hao) in an enriched consortium inoculated with humic acid were determined. Notably, the maximum nitrification rate value was observed at 315 mg-N·l-1 of ammonium, but the highest functional gene copy numbers were obtained at 630 mg-N·l-1 of ammonium. No inhibition of the nitrification rate and functional gene copy numbers was observed via the added nitrites. The optimum temperature for maximum nitrification performance was observed to be 30℃. The amoA-bac copy numbers were also greater than those of amoA-arc under all test conditions. Notably, amoA-arc copy numbers and nitrification efficiency showed a positive relationship in network analysis. These results indicate that ammonium-oxidizing archaea and bacteria play important roles in the nitrification process.
Abstract
In this study, the effects of ammonium concentration (117.5−1155.0 mg-N·l-1), nitrite concentration (0-50.0 mg-N·l-1), and temperature (15−35℃) on nitrification performance and its functional genes (amoA-arc, amoA-bac, hao) in an enriched consortium inoculated with humic acid were determined. Notably, the maximum nitrification rate value was observed at 315 mg-N·l-1 of ammonium, but the highest functional gene copy numbers were obtained at 630 mg-N·l-1 of ammonium. No inhibition of the nitrification rate and functional gene copy numbers was observed via the added nitrites. The optimum temperature for maximum nitrification performance was observed to be 30℃. The amoA-bac copy numbers were also greater than those of amoA-arc under all test conditions. Notably, amoA-arc copy numbers and nitrification efficiency showed a positive relationship in network analysis. These results indicate that ammonium-oxidizing archaea and bacteria play important roles in the nitrification process.
참고문헌 (31)
- Levy-Booth DJ, "Microbial functional genes involved in nitrogen fixation, nitrification and denitrification in forest ecosystems", Soil Biol. Biochem, 75, pp.11~25 (2014)
- Bru D, "Determinants of the distribution of nitrogen-cycling microbial communities at the landscape scale", ISME J, 5, pp.532~542 (2011)
- Horz H-P, "Ammoniaoxidizing bacteria respond to multifactorial global change", Proc. Natl. Acad. Sci. USA, 101, pp.15136~15141 (2004)
- Gao J, "Abundance and diversity based on amoA genes of ammonia-oxidizing archaea and bacteria in ten wastewater treatment systems", Appl. Microbiol. Biotechnol, 98, pp.3339~3354 (2014)
- Keluskar R, "Evaluation of hydroxylamine oxidoreductase as a functional and phylogenetic marker to differentiate Nitrosomonas spp", J. Basic Microbiol, 54, pp.261~268 (2014)
- Chen S, "Nitrification kinetics of biofilm as affected by water quality factors", Aquacult. Eng, 34, pp.34179~34197 (2006)
- Shanahan JW, "Alkalinity and pH effects on nitrification in a membrane aerated bioreactor: An experimental and model analysis", Water Res, 74, pp.10~22 (2015)
- Kinyage JP, "Impact of temperature on ammonium and nitrite removal rates in RAS moving bed biofilters", Aquacult. Eng, 75, pp.51~55 (2016)
- Paerl HW, "Techniques in microbial ecology", pp.3~30 (1998), Oxford University Press
- Kim TG, "Effects of water temperature and backwashing on bacterial population and community in a biological activated carbon process at a water treatment plant", Appl. Microbiol. Biotechnol, 98, pp.1417~1427 (2014)
- Song K, "Abundance, transcription levels and phylogeny of bacteria capable of nitrous oxide reduction in a municipal wastewater treatment plant", J. Biosci. Bioeng, 118, pp.289~297 (2014)
- Francis CA, "Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean", Proc. Natl. Acad. Sci. USA, 102, pp.14683~14688 (2005)
- Rotthauwe J, "The Ammonia Monooxygenase structural gene amoA as a functional marker?: molecular finescale analysis of natural ammonia-oxidizing populations", Appl. Environ. Microbiol, 63, pp.4704~4712 (1997)
- Wu B, "Ecological and enzymatic responses to petroleum contamination", Environ. Sci. Process. Impacts, 16, pp.1501~1509 (2014)
- Schmid MC, "Environmental detection of octaheme cytochrome c hydroxylamine", hydrazine oxidoreductase genes of aerobic and anaerobic ammonium-oxidizing bacteria, 2008, pp.10, v3140~3149
- Padhi SK, "Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater", Int. Biodeterior. Biodegrad, 78, pp.67~73 (2013)
- Ki BM, "Extended local similarity analysis (eLSA) reveals unique associations between bacterial community structure and odor emission during pig carcasses decomposition. J. Environ. Sci. Health A Tox", Hazard. Subst. Environ. Eng, 53, pp.718~727 (2018)
- Ruiz G, "Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration", Water Res, 37, pp.1371~1377 (2003)
- Aslan S, "Ammonium oxidation via nitrite accumulation under limited oxygen concentration in sequencing batch reactors", Bioresour. Technol, 100, pp.659~664 (2009)
- Wang LK, "Operation of partial nitrification to nitrite of landfill leachate and its performance with respect to different oxygen conditions", Biochem. Eng. J, 87, pp.62~68 (2014)
- Leininger S, "Archaea predominate among ammonia-oxidizing prokaryotes in soils", Nature, 442, pp.806~809 (2006)
- He JZ, "Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices", Environ. Microbiol, 9, pp.2364~2374 (2007)
- Sterngren AE, "Archaeal ammonia oxidizers dominate in numbers, but bacteria drive gross nitrification in N-amended grassland soil", Front. Microbiol, 6, pp.1350~ (2015)
- Gao J, "Changes of abundance and diversity of ammonia-oxidizing archaea (AOA) and bacteria (AOB) in three nitrifying bioreactors with different ammonia concentrations", Desalin. Water Treat, 57, pp.21463~21475 (2016)
- Van Cleemput O, "Nitrite in soils: accumulation and role in the formation of gaseous N compounds", Fertil. Res, 45, pp.81~89 (1995)
- Verstraete W, "Advances in microbial ecology", pp.135~214 (1997), Springer US
- Jones RD, "Effects of temperature, pH, salinity, and inorganic nitrogen on the rate of ammonium oxidation by nitrifiers isolated from wetland environments", Microb. Ecol, 6, pp.339~347 (1980)
- Antoniou P, "Effect of temperature and pH on the effective maximum specific growth rate of nitrifying bacteria", Water Res, 24, pp.97~101 (1990)
- Park HD, "Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors", Appl. Environ. Microbiol, 72, pp.5643~5647 (2006)
- Limpiyakorn T, "Abundance of amoA genes of ammonia-oxidizing archaea and bacteria in activated sludge of full-scale wastewater treatment plants", Bioresour. Technol, 102, pp.3694~3701 (2011)
- Sinthusith N, "Identification and quantification of bacteria and archaea responsible for ammonia oxidation in different activated sludge of full-scale wastewater treatment plants", J. Environ. Sci. Heal. - Part A Toxic Hazardous Subst. Environ. Eng, 50, pp.169~175 (2015)