Biotransformation of Ginsenosides by Eoyukjangderived Lactic Acid Bacteria in Mountain-cultivated Ginseng
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Biotransformation of ginsenosides by microorganisms alters the absorption and bioavailability of ginseng as a medicinal herb. In this study, we isolated two kinds of fermenting microorganisms from Eoyukjang, which is a traditional Korean fermented food made from soybean. Next, we identified and detected their ability to convert major ginsenosides to compound K. The two microorganisms, referred to as R2-6 and R2- 15, had 100% similarity with Lactobacillus plantarum subsp. plantarum ATCC 14917T and Lactobacillus rhamnosus JCM 1136T, respectively. The optimal pH and growth temperature of the isolates were determined to be pH 6-7 and 30℃. After fermentation for 30 days, the major ginsenosides in the mountain-cultivated ginseng were transformed to the highly bioactive ginsenoside, compound K, in the final product.
Abstract
Biotransformation of ginsenosides by microorganisms alters the absorption and bioavailability of ginseng as a medicinal herb. In this study, we isolated two kinds of fermenting microorganisms from Eoyukjang, which is a traditional Korean fermented food made from soybean. Next, we identified and detected their ability to convert major ginsenosides to compound K. The two microorganisms, referred to as R2-6 and R2- 15, had 100% similarity with Lactobacillus plantarum subsp. plantarum ATCC 14917T and Lactobacillus rhamnosus JCM 1136T, respectively. The optimal pH and growth temperature of the isolates were determined to be pH 6-7 and 30℃. After fermentation for 30 days, the major ginsenosides in the mountain-cultivated ginseng were transformed to the highly bioactive ginsenoside, compound K, in the final product.
참고문헌 (26)
- Choi KT, "Botanical characteristics, pharmacological effects and medicinal components of Korean Panax ginseng C. A. Meyer", Acta Pharmacol. Sin, 29, pp.1109~1118 (2008)
- 이상명, "Characterization of Korean Red Ginseng (Panax ginseng Meyer): History, preparation method, and chemical composition", Journal of Ginseng Research, 39(4), pp.384~391 (2015), 고려인삼학회
- Hasegawa H, "Proof of the mysterious efficacy of ginseng: basic and clinical trials: metabolic activation of ginsenoside: deglycosylation by intestinal bacteria and esterification with fatty acid", J. Pharmacol. Sci, 95, pp.153~157 (2004)
- Padmanaban Mohanan, "Molecular signaling of ginsenosides Rb1, Rg1, and Rg3 and their mode of actions", Journal of Ginseng Research, 42(2), pp.123~132 (2018), 고려인삼학회[DOI]
- 이창호, "A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases", Journal of Ginseng Research, 38(3), pp.161~166 (2014), 고려인삼학회
- Wang W, "In vitro anti-cancer activity and structure-activity relationships of natural products isolated from fruits of Panax ginseng", Cancer Chemother. Pharmacol, 59, pp.589~601 (2007)
- Hwang YP, "Ginsenoside Rb1 protects against 6- hydroxydopamine-induced oxidative stress by increasing heme oxygenase-1 expression through an estrogen receptor-related PI3K", Akt, Toxicol. Appl. Pharmacol, pp.2010, 242[DOI]
- 양우석, "Nuclear factor kappa-B- and activator protein-1-mediated immunostimulatory activity of compound K in monocytes and macrophages", Journal of Ginseng Research, 41(3), pp.298~306 (2017), 고려인삼학회[DOI]
- Nag SA, "Ginsenosides as anticancer agents: In vitro and in vivo activities, structure-activity relationships, and molecular mechanisms of action", Front. Pharmacol, 3, pp.25~ (2012)
- Choonsig Kim, "Soil properties of cultivation sites for mountain-cultivated ginseng at local level", Journal of Ginseng Research, 39(1), pp.76~80 (2015), 고려인삼학회
- Kim EO, "Bioavailability of ginsenosides from white and red ginsengs in the simulated digestion model", J. Agric. Food Chem, 62, pp.10055~10063 (2014)
- Bae EA, "Metabolism of 20(S)- and 20(R)-ginsenoside Rg3 by human intestinal bacteria and its relation to in vitro biological activities", Biol. Pharm. Bull, 25, pp.58~63 (2002)
- 배은아, "Transformation of Ginseng Saponins to Ginsenoside Rh2 by Acids and Human Intestinal Bacteria and Biological Activities of Their Transformants", Archives of Pharmacal Research, 27(1), pp.61~67 (2004), 대한약학회
- 김세화, "Enzymatic Transformation of Ginsenoside Rb1 by Lactobacillus pentosus Strain 6105 from Kimchi", Journal of Ginseng Research, 36(3), pp.291~297 (2012), 고려인삼학회
- 박보연, "Evaluation of ginsenoside bioconversion of lactic acid bacteria isolated from kimchi", Journal of Ginseng Research, 41(4), pp.524~530 (2017), 고려인삼학회[DOI]
- 오유진, "어육장의 미생물학적 특성", 한국식품과학회지, 40(6), pp.656~660 (2008), 한국식품과학회
- Peralta RM, "A highly thermostable β-glucosidase activity from the thermophilic fungus Humicola grisea var. thermoidea: purification and biochemical characterization", FEMS Microbiol. Lett, 146, pp.291~295 (1997)
- Lee SH, "Viability in artificial gastric and bile juice and antimicrobial activity of some lactic acid bacteria isolated from Kimchi", J. Microbiol. Biotechnol, 6, pp.617~622 (1997)
- Kyung Hoon Chang, "Evaluation of glucosidases of Aspergillus niger strain comparing with other glucosidases in transformation of ginsenoside Rb1 to ginsenosides Rg3", Journal of Ginseng Research, 38(1), pp.47~51 (2014), 고려인삼학회[DOI]
- Helms S, "Cancer prevention and therapeutics: Panax ginseng", Altern. Med. Rev, 9, pp.259~274 (2004)
- Succi M, "Bile salt and acid tolerance of Lactobacillus rhamnosus strains isolated from Parmigiano Reggiano cheese", FEMS Microbiol. Lett, 244, pp.129~137 (2005)
- Hassanzadazar H, "Investigation of antibacterial, acid and bile tolerance properties of lactobacilli isolated from Koozeh cheese", Vet. Res. Forum, 3, pp.181~185 (2012)
- Jieun Jung, "Fermentation of red ginseng extract by the probiotic Lactobacillus plantarum KCCM 11613P: ginsenoside conversion and antioxidant effects", Journal of Ginseng Research, 43(1), pp.20~26 (2019), 고려인삼학회[DOI]
- Kim BG, "Fermentation of Korean red ginseng by Lactobacillus plantarum M-2 and its immunological activities", Appl. Biochem. Biotechnol, 165, pp.1107~1119 (2011)
- Seockmo Ku, "Whole-Cell Biocatalysis for Producing Ginsenoside Rd from Rb1 Using Lactobacillus rhamnosus GG", Journal of Microbiology and Biotechnology, 26(7), pp.1206~1215 (2016), 한국미생물·생명공학회[DOI]
- Wan JY, "Biotransformation and metabolic profile of American ginseng saponins with human intestinal microflora by liquid chromatography quadrupole time-of-flight mass spectrometry", J. Chromatogr. A, 1286, pp.83~92 (2013)
