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된장 제조를 위한 바로 사용 종균의 개발

Development of Ready-to-use Starters for the Production of doenjang

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초록

In Korea, traditional doenjang is manufactured using the conventional method at home and by small-scale enterprises. Because this age-old process depends on natural inoculation of various microorganisms, it is difficult to reproduce or maintain consistency in the final product quality across batches. Moreover, doenjang occasionally prepared by this method raises safety concerns related to aflatoxin, biogenic amine, and Bacillus cereus contamination. To develop starters that can be conveniently used at home or in small industry settings for the manufacturing of safe and flavor-improved doenjang, autochthonous microbe starters were developed in dried forms as ready-to-use starters. Each starter powder prepared by heat- or freeze-drying methods remained stable even after 24-week storage. These ready- to-use starter powders were successfully applied to lab-scale fermentation for the production of safe and flavor-improved doenjang. We believe that these ready-to-use starter powders will benefit small-scale enterprises in the manufacturing of doenjang of good reproducible quality.

Abstract

In Korea, traditional doenjang is manufactured using the conventional method at home and by small-scale enterprises. Because this age-old process depends on natural inoculation of various microorganisms, it is difficult to reproduce or maintain consistency in the final product quality across batches. Moreover, doenjang occasionally prepared by this method raises safety concerns related to aflatoxin, biogenic amine, and Bacillus cereus contamination. To develop starters that can be conveniently used at home or in small industry settings for the manufacturing of safe and flavor-improved doenjang, autochthonous microbe starters were developed in dried forms as ready-to-use starters. Each starter powder prepared by heat- or freeze-drying methods remained stable even after 24-week storage. These ready- to-use starter powders were successfully applied to lab-scale fermentation for the production of safe and flavor-improved doenjang. We believe that these ready-to-use starter powders will benefit small-scale enterprises in the manufacturing of doenjang of good reproducible quality.

참고문헌 (29)

  1. Chai C, "Determination of bioactive compounds in fermented soybean products using GC", MS and further investigation of correlation of their bioactivities, 2012, pp.880, 42~49
  2. 김병수, "Bacillus sp. SP-KSW3를 이용하여 제조한 된장 발효 과정중의 효소 활성 및 기능성의 변화", Food Science and Preservation, 14(5), pp.545~551 (2007), 한국식품저장유통학회
  3. Kim SH, "Isolation of angiotensin converting enzyme inhibitor from doenjang", Korean J. Food Sci. Technol, 31, pp.848~854 (1999)
  4. Lee S, "Comparative evaluation of microbial diversity and metabolite profiles in doenjang, a fermented soybean paste, during the two different industrial manufacturing processes", Food Chem, 221, pp.1578~1586 (2017)
  5. Lee SY, "Primary and secondary metabolite profiling of doenjang, a fermented soybean paste during industrial processing", Food Chem, 165, pp.157~166 (2014)
  6. Shukla S, "Effect of novel starter culture on reduction of biogenic amines, quality improvement, and sensory properties of doenjang, a traditional Korean soybean fermented sauce variety", J. Food Sci, 80, pp.M1794~M1803 (2015)
  7. Singh BP, "Functional significance of bioactive peptides derived from soybean", Peptides, 54, pp.171~179 (2014)
  8. 정도원, "Bacterial Community Migration in the Ripening of Doenjang, a Traditional Korean Fermented Soybean Food", Journal of Microbiology and Biotechnology, 24(5), pp.648~660 (2014), 한국미생물·생명공학회[DOI]
  9. Jung JY, "Microbial community dynamics during fermentation of doenjang-meju, traditional Korean fermented soybean", Int. J. Food Microbiol, 185, pp.112~120 (2014)
  10. 김대호, "Mycoflora of Soybeans Used for Meju Fermentation", Mycobiology, 41(2), pp.100~107 (2013), 한국균학회
  11. Lee JH, "Determination of microbial diversity in meju, fermented cooked soya beans, using nested PCR-denaturing gradient gel electrophoresis", Lett. Appl. Microbiol, 51, pp.388~394 (2010)
  12. Nam YD, "Microbial community analysis of Korean soybean pastes by next-generation sequencing", Int. J. Food Microbiol, 155, pp.36~42 (2012)
  13. Lee EJ, "Development of safe and flavor-rich doenjang (Korean fermented soybean paste) using autochthonous mixed starters at the pilot plant scale", J. Food Sci, 83, pp.1723~1732 (2018)
  14. 이진희, "Safety Evaluation of Filamentous Fungi Isolated from Industrial Doenjang Koji", Journal of Microbiology and Biotechnology, 24(10), pp.1397~1404 (2014), 한국미생물·생명공학회[DOI]
  15. Seong Eun Kang, "Distribution of poly-γ-glutamate (γ-PGA) producers in Koreanfermented foods, Cheongkukjang, Doenjang, and Kochujang", Food Science and Biotechnology, 14(5), pp.704~708 (2005), 한국식품과학회
  16. Jeong DW, "Safety assessment of Tetragenococcus halophilus isolates from doenjang, a Korean highsalt- fermented soybean paste", Food Microbiol, 62, pp.92~98 (2017)
  17. Kum SJ, "Effects of Aspergillus species inoculation and their enzymatic activities on the formation of volatile components in fermented soybean paste (doenjang)", J. Agric. Food Chem, 63, pp.1401~1418 (2015)
  18. Choi IS, "Method for increasing viability, storage stability, acid tolerance or oxgall tolerance of lactic acid bacteria. Republic of Korea patent no. 1016055160000", ~ (2016)
  19. Chung M-J, "Manufacturing method of protein-coated lactic acid bacteria powder. Republic of Korea patent no. 1004294940000", ~ (2004)
  20. Intrexon Actobiotics NV, "Cryoprotectants for freeze drying of lactic acid bacteria. Republic of Korea patent no. 1018214170000", ~ (2018)
  21. Beak NS, "Manufacturing method of rice Kefir by using Lactobacillus plantarum and Saccharomyces cerevisiae. Republic of Korea patent no. 1013131200000", ~ (2013)
  22. Blanquet S, "Effects of cryoprotectants on the viability and activity of freeze dried recombinant yeasts as novel oral drug delivery systems assessed by an artificial digestive system", Eur. J. Pharm. Biopharm, 61, pp.32~39 (2005)
  23. Coutinho C, "Trehalose as cryoprotectant for preservation of yeast strains", J. Biotechnol, 7, pp.23~32 (1988)
  24. Krishna C, "Banana waste as substrate for α-amylase by Bacillus subtilis (CBTK 106) under solid-state fermentation", Appl. Microbiol. Biotechnol, 46, pp.106~111 (1996)
  25. Rhee CH, "Changes of microorganisms, enzyme activity and physiological functionality in the traditional deonjang with various concentrations of Lentinus edodes during fermentation", Appl. Biol. Chem, 43, pp.277~284 (2000)
  26. Kappes RM, "Response of Bacillus subtilis to high osmolarity: uptake of carnitine, crotonobetaine and gamma-butyrobetaine via the ABC transport system OpuC", Microbiology, 144, pp.83~90 (1998)
  27. Jung WY, "Functional characterization of bacterial communities responsible for fermentation of doenjang: A traditional Korean fermented soybean paste", Front. Microbiol, 7, pp.827~ (2016)
  28. Kim HG, "Sensory characteristics and consumer acceptability of fermented soybean paste (Doenjang)", J. Food Sci, 75, pp.S375~S383 (2010)
  29. Nelson G, "An amino-acid taste receptor", Nature, 416, pp.199~202 (2002)

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