유해균 억제능을 지닌 복합종균을 접종하여 제조한 된장에서 Bacillus cereus 억제
Inhibition of Bacillus cereus in Doenjang Fermented with Multiple Starters Showing Inhibitory Activity against Pathogens
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초록
Doenjang samples were prepared by inoculation of multiple starters consisting of two Bacillus spp., one yeast, and one fungus. Doenjang A was fermented with Bacillus amyloliquefaciens EMD17, B. amyloliquefaciens MJ1-4, Pichia farinosa SY80, and Rhizopus oryzae. Doenjang B and C were fermented with the same yeast and fungus but different Bacillus strains; namely, B. amyloliquefaciens EMD17 and B. subtilis CH3-5 for doenjang B, and B. amyloliquefaciens MJ1-4 and B. subtilis CH3-5 for doenjang C. Doenjang D was fermented with microorganisms present in rice straw (control). The doenjang samples were spiked with B. cereus ATCC14579 at two different levels, 104 CFU/g doenjang (I) and 107 CFU/g doenjang (II). All eight doenjang samples were fermented for 70 days at 25°C. Growth of B. cereus was inhibited in doenjang A, B, and C, with the bacterial cell count after 70 days being less than the initial 104 CFU/g added, whereas B. cereus was not inhibited in doenjang D. Doenjang B showed the strongest inhibitory activity against B. cereus, with a cell count of less than 103 CFU/g after 42 days, even when B. cereus was initially added at 107 CFU/g. Some properties of the doenjang samples, such as pH, TA, and amino-type nitrogen content, were similar to those of doenjang fermented with starters only. The results indicate that carefully selected starters can effectively prevent the growth of B. cereus during doenjang fermentation.
Abstract
Doenjang samples were prepared by inoculation of multiple starters consisting of two Bacillus spp., one yeast, and one fungus. Doenjang A was fermented with Bacillus amyloliquefaciens EMD17, B. amyloliquefaciens MJ1-4, Pichia farinosa SY80, and Rhizopus oryzae. Doenjang B and C were fermented with the same yeast and fungus but different Bacillus strains; namely, B. amyloliquefaciens EMD17 and B. subtilis CH3-5 for doenjang B, and B. amyloliquefaciens MJ1-4 and B. subtilis CH3-5 for doenjang C. Doenjang D was fermented with microorganisms present in rice straw (control). The doenjang samples were spiked with B. cereus ATCC14579 at two different levels, 104 CFU/g doenjang (I) and 107 CFU/g doenjang (II). All eight doenjang samples were fermented for 70 days at 25°C. Growth of B. cereus was inhibited in doenjang A, B, and C, with the bacterial cell count after 70 days being less than the initial 104 CFU/g added, whereas B. cereus was not inhibited in doenjang D. Doenjang B showed the strongest inhibitory activity against B. cereus, with a cell count of less than 103 CFU/g after 42 days, even when B. cereus was initially added at 107 CFU/g. Some properties of the doenjang samples, such as pH, TA, and amino-type nitrogen content, were similar to those of doenjang fermented with starters only. The results indicate that carefully selected starters can effectively prevent the growth of B. cereus during doenjang fermentation.
참고문헌 (18)
- Bae SI, "Survey of aflatoxin B1 in domestic doenjang and kochujang determined by enzyme linked-immunosorbent assay", J. Fd. Hyg. Safety, 18, pp.95~100 (2003)
- 차연수, "Doenjang, a Korean Fermented Soy Food, Exerts Antiobesity and Antioxidative Activities in Overweight Subjects with the PPAR-c2 C1431T Polymorphism: 12-Week, Double-Blind Randomized Clinical Trial", Journal of Medicinal Food, 17(1), pp.119~127 (2014), 한국식품영양과학회[DOI]
- 장미, "Bacillus subtilis DJI을 이용하여 제조된 세균형 코지와 속성된장의 특성", 한국미생물·생명공학회지, 35(4), pp.325~333 (2007), 한국미생물·생명공학회
- 조민정, "Microbial and Physiochemical Properties of Cheonggukjang Fermented Using Bacillus Strains with Antibacterial or Antifungal Activities", Food Science and Biotechnology, 23(5), pp.1525~1532 (2014), 한국식품과학회[DOI]
- 조민정, "Properties of Doenjang (Soybean Paste) Fermented with Multiple Starters", 농업생명과학연구, 48(6), pp.291~300 (2014), 농업생명과학연구원[DOI]
- 조민정, "Properties of Meju Fermented with Multiple Starters", 한국미생물·생명공학회지, 44(2), pp.109~116 (2016), 한국미생물·생명공학회[DOI]
- Choi SY, "Growth suppression effect of traditional fermented soybean paste (doenjang) on the various tumor cells", J. Korean Soc. Food Sci. Nutr, 28, pp.458~463 (1999)
- Hwang JH, "Angiotension I converting enzyme inhibitory effect of doenjang fermented by B. subtilis SCB-3 isolated from meju, Korean traditional food", J. Korean Soc. Food Sci. Nutr, 26, pp.775~783 (1997)
- 정선주, "Cloning of Fibrinolytic Enzyme Gene from Bacillus subtilis Isolated from Cheonggukjang and Its Expression in Protease-deficient Bacillus subtilis Strains", Journal of Microbiology and Biotechnology, 17(6), pp.1018~1023 (2007), 한국미생물·생명공학회
- Korea Food and Drug Administration, "Food standards codex I", KFDA, 2-VI-6~ (2011)
- Ko BK, "Metabolomic assessment of fermentative capability of soybean starter treated with high pressure", J. Agric. Food Chem, 58, pp.8738~8747 (2010)
- 이은실, "Bacillus cereus에 대한 길항적 저해 작용과 biogenic amines 분해 능력을지닌 Bacillus licheniformis SCK A08 균의 특성", 한국식품위생안전성학회지, 29(1), pp.40~46 (2014), 한국식품위생안전성학회
- Hwang A Lee, "Isolation of Bacillus amyloliquefaciens Strains with Antifungal Activities from Meju", Preventive Nutrition and Food Science, 17(1), pp.64~70 (2012), 한국식품영양과학회[DOI]
- 이학태, "재래 된장과 시판 된장의 미생물 오염 및 바이오제닉 아민 함량 분석", 한국식품위생안전성학회지, 24(1), pp.102~109 (2009), 한국식품위생안전성학회
- 이주연, "저염 된장 발효 중 이화학적 특성 변화", 산업식품공학, 14(2), pp.153~158 (2010), 한국산업식품공학회
- 이재용, "Antimicrobial Activity of Bacillus amyloliquefaciens EMD17 Isolated from Cheonggukjang and Potential Use as a Starter for Fermented Soy Foods", Food Science and Biotechnology, 25(2), pp.525~532 (2016), 한국식품과학회[DOI]
- Park SK, "Quality characteristics of home- made doenjang, a traditional Korean soybean paste", Korean J. Soc. Food Sci, 16, pp.121~127 (2000)
- Shin DH, "Globalization of Korean fermented soybean products", Food Ind. Nutr, 11, pp.19~24 (2008)