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Molecular Characterization of Protease Producing Idiomarina Species Isolated from Peruvian Saline Environments

Carol N. Flores-Fernández1|Elizabeth Chávez-Hidalgo|Marco Santos|Amparo I. Zavaleta|David R. Arahal
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2019.09|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제47권 제3호|pp. 401-411|DOI 10.4014/mbl.1809.09001

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키워드

proteaseIdiomarinaPeruvian saline environments16S-23S ITStRNA
주제분류생물학

초록

All Idiomarina species are isolated from saline environments; microorganisms in such extreme habitats develop metabolic adaptations and can produce compounds such as proteases with an industrial potential. ARDRA and 16S rRNA gene sequencing are established methods for performing phylogenetic analysis and taxonomic identification. However, 16S-23S ITS is more variable than the 16S rRNA gene within a genus, and is therefore, used as a marker to achieve a more precise identification. In this study, ten protease producing Idiomarina strains isolated from the Peruvian salterns were characterized using biochemical and molecular methods to determine their bacterial diversity and industrial potential. In addition, comparison between the length and nucleotide sequences of a 16S-23S ITS region allowed us to assess the inter and intraspecies variability. Based on the 16S rRNA gene, two species of Idiomarina were identified (I. zobellii and I. fontislapidosi). However, biochemical tests revealed that there were differences between the strains of the same species. Moreover, it was found that the ITS contains two tRNA genes, tRNAIle (GAT) and tRNAAla (TGC), which are separated by an ISR of a variable size between strains of I. zobellii. In one strain of I. zobellii (PM21), we found nonconserved nucleotides that were previously not reported in the tRNAAla gene sequences of Idiomarina spp. Thus, based on the biochemical and molecular characteristics, we can conclude that protease producing Idiomarina strains have industrial potential; only two I. zobellii strains (PM48 and PM72) exhibited the same properties. The differences between the other strains could be explained by the presence of subspecies.

Abstract

All Idiomarina species are isolated from saline environments; microorganisms in such extreme habitats develop metabolic adaptations and can produce compounds such as proteases with an industrial potential. ARDRA and 16S rRNA gene sequencing are established methods for performing phylogenetic analysis and taxonomic identification. However, 16S-23S ITS is more variable than the 16S rRNA gene within a genus, and is therefore, used as a marker to achieve a more precise identification. In this study, ten protease producing Idiomarina strains isolated from the Peruvian salterns were characterized using biochemical and molecular methods to determine their bacterial diversity and industrial potential. In addition, comparison between the length and nucleotide sequences of a 16S-23S ITS region allowed us to assess the inter and intraspecies variability. Based on the 16S rRNA gene, two species of Idiomarina were identified (I. zobellii and I. fontislapidosi). However, biochemical tests revealed that there were differences between the strains of the same species. Moreover, it was found that the ITS contains two tRNA genes, tRNAIle (GAT) and tRNAAla (TGC), which are separated by an ISR of a variable size between strains of I. zobellii. In one strain of I. zobellii (PM21), we found nonconserved nucleotides that were previously not reported in the tRNAAla gene sequences of Idiomarina spp. Thus, based on the biochemical and molecular characteristics, we can conclude that protease producing Idiomarina strains have industrial potential; only two I. zobellii strains (PM48 and PM72) exhibited the same properties. The differences between the other strains could be explained by the presence of subspecies.

참고문헌 (57)

  1. Ivanova EP, "Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov", Int. J. Syst. Evol. Microbiol, 50, pp.901~907 (2000)
  2. Ivanova EP, "Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov", Int. J. Syst. Evol. Microbiol, 54, pp.1773~1788 (2004)
  3. Jean WD, "Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae", Int. J. Syst. Evol. Microbiol, 56, pp.899~905 (2006)
  4. Hu ZY, "Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China", Int. J. Syst. Evol. Microbiol, 57, pp.2572~2577 (2007)
  5. Wu YH, "Pseudidiomarina donghaiensis sp. nov. and Pseudidiomarina maritima sp. nov., isolated from the East China Sea", Int. J. Syst. Evol. Microbiol, 59, pp.1321~1325 (2009)
  6. Jean WD, "Pseudidiomarina marina sp. nov. and Pseudidiomarina tainanensis sp. nov. and reclassification of Idiomarina homiensis and Idiomarina salinarum as Pseudidiomarina homiensis comb. nov. and Pseudidiomarina salinarum comb. nov., respectively", Int. J. Syst. Evol. Microbiol, 59, pp.53~59 (2009)
  7. Park SC, "Pseudidiomarina aestuarii sp. nov., a marine bacterium isolated from shallow coastal seawater", Int. J. Syst. Evol. Microbiol, 60, pp.2071~2075 (2010)
  8. Taborda M, "Description of Idiomarina insulisalsae sp. nov., isolated from the soil of a sea salt evaporation pond, proposal to transfer the species of the genus Pseudidiomarina to the genus Idiomarina and emended description of the genus Idiomarina", Syst. Appl. Microbiol, 32, pp.371~378 (2009)
  9. Wang L, "Idiomarina xiamenensis sp. nov., isolated from surface seawater, and proposal to transfer Pseudidiomarina aestuarii to the genus Idiomarina as Idiomarina aestuarii comb. nov", Int. J. Syst. Evol. Microbiol, 61, pp.969~973 (2011)
  10. Martínez-Cánovas MJ, "Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain", Int. J. Syst. Evol. Microbiol, 54, pp.1793~1797 (2004)
  11. Chen MH, "Idiomarina aquimaris sp. nov., isolated from the reef-building coral Isopora palifera", Int. J. Syst. Evol. Microbiol, 62, pp.1536~1542 (2012)
  12. Du J, "Idiomarina atlantica sp. nov., a marine bacterium isolated from the deep sea sediment of the North Atlantic Ocean", Antonie Van Leeuwenhoek, 107, pp.393~401 (2014)
  13. Lee JC, "Idiomarina halophila sp. nov., isolated from a solar saltern sediment", Int. J. Syst. Evol. Microbiol, 65, pp.1268~1273 (2015)
  14. Kwon SW, "Idiomarina homiensis sp. nov., isolated from seashore sand in Korea", Int. J. Syst. Evol. Microbiol, 56, pp.2229~2233 (2006)
  15. Poddar A, "Comparative analysis of 16S rRNA signature sequences of the genus Idiomarina and Idiomarina woesei sp. nov., a novel marine bacterium isolated from the Andaman Sea", Res. Microbiol, 165, pp.501~507 (2014)
  16. Rohban R, "Screening and isolation of halophilic bacteria producing extracellular hydrolyses from Howz Soltan Lake, Iran", J. Ind. Microbiol. Biotechnol, 36, pp.333~340 (2009)
  17. Babavalian H, "Isolation and identification of moderately halophilic bacteria producing hydrolytic enzymes from the largest hypersaline playa in Iran", Microbiology, 82, pp.466~474 (2013)
  18. Kumar S, "Screening and isolation of halophilic bacteria producing industrially important enzymes", Braz. J. Microbiol, 43, pp.1595~1603 (2012)
  19. Sánchez-Porro C, "Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes", J. Appl. Microbiol, 94, pp.295~300 (2003)
  20. El Hadj-Ali N, "Biochemical and molecular characterization of a detergent stable alkaline serine-protease from a newly isolated Bacillus licheniformis NH1", Enzyme Microb. Technol, 40, pp.515~523 (2007)
  21. Moreno ML, "Halophilic bacteria as a source of novel hydrolytic enzymes", Life, 3, pp.38~51 (2013)
  22. Contesini FJ, "An overview of Bacillus proteases: from production to application", Crit. Rev. Biotechnol, 38, pp.321~334 (2018)
  23. Yan Xin, "Purification and Characterization of a Novel Extracellular Thermostable Alkaline Protease from Streptomyces sp. M30", Journal of Microbiology and Biotechnology, 25(11), pp.1944~1953 (2015), 한국미생물·생명공학회[DOI]
  24. Selvaraj Indhuja, "Isolation and Characterization of a Feather Degrading Alkalophilic Streptomyces sp. TBG-S13A5 and its Keratinolytic Properties", 한국미생물·생명공학회지, 40(4), pp.303~309 (2012), 한국미생물·생명공학회[DOI]
  25. Sivaprakasam S, "Optimization studies on production of a salt-tolerant protease from Pseudomonas aeruginosa strain BC1 and its application on tannery saline wastewater treatment", Braz. J. Microbiol, 42, pp.1506~1515 (2011)
  26. Tokajian S, "16S-23S rRNA Gene Intergenic Spacer Region Variability Helps Resolve Closely Related Sphingomonads", Front Microbiol, 7, pp.149~ (2016)
  27. Osorio CR, "Variation in 16S-23S rRNA intergenic spacer regions in Photobacterium damselae:a mosaic-like structure", Appl. Environ. Microbiol, 71, pp.636~645 (2005)
  28. Conrads G, "16S-23S rDNA internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus Fusobacterium", Int. J. Syst. Evol. Microbiol, 52, pp.493~499 (2002)
  29. Liguori AP, "Diversity of 16S-23S rDNA internal transcribed spacer (ITS) reveals phylogenetic relationships in Burkholderia pseudomallei and its near-neighbors", PLoS One, 6, pp.e29323~ (2011)
  30. Bauer AW, "Antibiotic susceptibility testing by a standardized single diffusion method", Am. J. Clin. Pathol, 45, pp.493~496 (1966)
  31. Hou S, "Genome sequence of the deep-sea γ-proteobacterium Idiomarina loihiensis reveals amino acid fermentation as a source of carbon and energy", Proc. Natl. Acad. Sci. USA, 101, pp.18036~18041 (2004)
  32. Dodia MS, "Characterization and stability of extracellular alkaline proteases from halophilic and alkaliphilic bacteria isolated from saline habitat of coastal Gujarat, India. Braz", J. Microbiol, 37, pp.276~282 (2006)
  33. Pant G, "Production, optimization and partial purification of protease from Bacillus subtilis", J. Taibah. Univ. Sci, 9, pp.50~55 (2015)
  34. Singh SK, "An antibiotic, heavy metal resistant and halotolerant Bacillus cereus SIU1 and its thermoalkaline protease", Microb. Cell Fact, 59, pp.1~7 (2010)
  35. Suganthi C, "Screening and optimization of protease production from a halotolerant Bacillus licheniformis isolated from saltern sediments", Genet. Eng. Biotechnol. J, 11, pp.47~52 (2013)
  36. Zhou C, "A novel alkaline protease from alkaliphilic Idiomarina sp. C9-1 with potential application for eco-friendly enzymatic dehairing in the leather industry", Sci. Rep, 8, pp.16467~ (2018)
  37. Stewart FJ, "Intragenomic variation and evolution of the internal transcribed spacer of the rRNA operon in bacteria", J. Mol. Evol, 65, pp.44~67 (2007)
  38. Singh V, "Molecular assessment of 16S-23S rDNA internal transcribed spacer length polymorphism of Aeromonas hydrophila", Adv. Microbiol, 2, pp.72~78 (2012)
  39. Brettar I, "Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea", Int. J. Syst. Evol. Microbiol, 53, pp.407~413 (2003)
  40. Donachie SP, "Idiomarina loihiensis sp. nov., a halophilic γ-Proteobacterium from the Lō ‘ihi submarine volcano, Hawai‘i", Int. J. Syst. Evol. Microbiol, 53, pp.1873~1879 (2003)
  41. Choi DH, "Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea", Int. J. Syst. Evol. Microbiol, 55, pp.379~383 (2005)
  42. Yoon JH, "Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea", Int. J. Syst. Evol. Microbiol, 57, pp.2503~2506 (2007)
  43. Zhang YJ, "Idiomarina maris sp. nov., a marine bacterium isolated from sediment", Int. J. Syst. Evol Microbiol, 62, pp.370~375 (2012)
  44. Sitdhipol J, "Idiomarina piscisalsi sp. nov., from fermented fish (pla-ra)in Thailand", J. Gen. Appl. Microbiol, 59, pp.385~391 (2013)
  45. Song L, "Idiomarina indica sp. nov., isolated from seawater", Int. J. Syst. Evol. Microbiol, 63, pp.2497~2500 (2013)
  46. Zhong ZP, "Idiomarina planktonica sp. nov., isolated from a saline lake", Int. J. Syst. Evol. Microbiol, 64, pp.3411~3416 (2014)
  47. León MJ, "Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns", Int. J. Syst. Evol. Microbiol, 65, pp.4595~4600 (2015)
  48. Hameed A, "Idiomarina tyrosinivorans sp. nov., isolated from estuarine surface water", Int. J. Syst. Evol. Microbiol, 66, pp.5384~5391 (2016)
  49. Marathe SK, "Isolation, partial purification, biochemical characterization and detergent compatibility of alkaline protease produced by Bacillus subtilis, Alcaligenes faecalis and Pseudomonas aeruginosa obtained from sea water samples", J. Genet. Eng. Biotechnol, 16, pp.39~46 (2018)
  50. Ali N, "Molecular characterization and growth optimization of halo-tolerant protease producing Bacillus subtilis Strain BLK-1. 5isolated from salt mines of Karak, Pakistan", Extremophiles, 20, pp.395~402 (2016)
  51. Ibrahim AS, "Production of extracellular alkaline protease by new halotolerant alkaliphilic Bacillus sp. NPST-AK15 isolated from hyper saline soda lakes", Electron. J. Biotechnol, 18, pp.236~243 (2015)
  52. Rathod MG, "Wealth from waste: Optimized alkaline protease production from agro-industrial residues by Bacillus alcalophilus LW8 and its biotechnological applications", J. Taibah. Univ. Sci, 8, pp.307~314 (2014)
  53. Pathak AP, "Alkaline protease production, extraction and characterization from alkaliphilic Bacillus licheniformis KBDL4: a Lonar soda lake isolate", Indian J. Exp. Biol, 50, pp.569~576 (2012)
  54. Jellouli K, "Alkaline-protease from Bacillus licheniformis MP1 : purification, characterization and potential application as a detergent additive and for shrimp waste deproteinization", Process Biochem, 46, pp.1248~1256 (2011)
  55. Essghaier B, "High salt-tolerant protease from a potential biocontrol agent Bacillus pumilus M3-16", Ann. Microbiol, 59, pp.553~ (2009)
  56. Jian Yang, "Characterization of a Thermophilic Hemoglobin-degrading Protease from Streptomyces rutgersensis SCSIO 11720 and Its Application in Antibacterial Peptides Production", Biotechnology and Bioprocess Engineering, 20(1), pp.79~90 (2015), 한국생물공학회
  57. Manivasagan P, "Production, characterization and antioxidant potential of protease from Streptomyces sp. MAB18 using poultry wastes", Biomed. Res. Int, pp.1~12 (2013)

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