재조합 Corynebacterium glutamicum으로부터 헴첼 생산에 미치는 프로모터의 효과
Effect of Promoters on the Heme Production in a Recombinant Corynebacterium glutamicum
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We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase (hemA+) under control of a constitutive promoter (P180) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the hemA+ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The hemA+ expression by PgapA (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by PH36 (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control (hemA+ expression by P180 constitutive promoter). The hemA+ expression by a late log-phase activating Psod (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The hemA+ expression led by a heat-shock responding chaperone promoter (PdnaK) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter stren
Abstract
We published that bacterial heme was over-produced in a recombinant Corynebacterium glutamicum expressing 5-aminolevulinic acid synthase (hemA+) under control of a constitutive promoter (P180) and the heme-producing C. glutamicum had commercial potentials; as an iron feed additive for swine and as a preservative for lactic acid bacteria. To enhance the heme production, the hemA+ gene was expressed under controls of various promoters in the recombinant C. glutamicum. The hemA+ expression by PgapA (a constitutive glycolytic promoter of glyceraldehyde-3-phosphate dehydrogenase) led 75% increase of heme production while the expression by PH36 (a constitutive, very strong synthetic promoter) resulted in 50% decrease compared with the control (hemA+ expression by P180 constitutive promoter). The hemA+ expression by a late log-phase activating Psod (an oxidative-stress responding promoter of superoxide dismutase) led 50% greater heme production than the control. The hemA+ expression led by a heat-shock responding chaperone promoter (PdnaK) resulted in 121% increase of heme production at the optimized heat-shock conditions. The promoter stren
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