본문 바로가기
KERD
논문KCI 등재

김치에서 분리된 젖산균의 β-glucuronidase 활성 탐색

Exploration of β-Glucuronidase Activity of Lactic Acid Bacteria Isolated from Kimchi

0

조회

0

다운로드

4

인용

0

참고문헌

키워드

주제분류생물학

초록

전통발효식품인 김치에서 분리된 젖산균으로부터 β- glucuronidase 효소활성이 높은 균주를 선발하였다. 김치에서 분리된 156점의 젖산균 중 52점의 균주가 glucuronic acid를 탄소원으로 대사하였으며, 대부분의 젖산균은 세포내β-glucuronidase 활성이 세포외 활성보다 유의적으로 높았다. 순무김치에서 분리된 Leu. mesenteroides KFRI 73007 균주가 0.77 ± 0.01 U/mg protein로서 가장 높은 세포내 β- glucuronidase 효소활성을 나타내었다. 최적 반응조건은 pH 7, 37℃이었으며 1.14 ± 0.01 U/mg protein의 효소활성을 나타냈다. 양이온 금속이온은 β-glucuronidase 효소활성을 약70% 이상 저해하였으며, 균주 배양에 사용한 탄소원 중 β- glucuronidase 생산을 위한 최적의 탄소원은 glucuronic acid이었다.

Abstract

Lactic acid bacteria (LAB) isolated from kimchi were studied for their β-glucuronidase activity. Among the 156 strains tested, 52 strains utilized glucuronic acid as a carbon source and their intracellular β-glucuronidase activities were significantly higher than their extracellular activities. Leuconostoc mesenteroides KFRI 73007 isolated from turnip kimchi exhibited the highest intracellular β-glucuronidase activity of 0.77 ± 0.01 U/mg protein, which was further increased to 1.14 ± 0.01 U/mg protein under optimized reaction conditions (pH 7, 37℃). The activity of β-glucuronidase was notably decreased by the addition of divalent cations, and glucuronic acid was the best carbon source to produce β-glucuronidase in Leu. mesenteroides KFRI 73007.

참고문헌 (36)

  1. Cheigh HS, "Biochemical, microbiological, and nutritional aspects of kimchi (Korean fermented vegetable products)", Crit. Rev. Food Sci. Nutr., 34, pp.175~203 (1994)
  2. Son KH, "Types and usage of kimchi", J. Korean Soc. Food Cult, 6, pp.503~520 (1991)
  3. 이은화, "김치냉장고의 숙성 후 저장 및 저온 저장 모드에서 6개월간 저장한 김장 김치의 발효특성 비교", 한국식품영양과학회지, 41(11), pp.1619~1625 (2012), 한국식품영양과학회
  4. Lee CH, "Kimchi; Korean fermented vegetable foods", Korean J. Diet Cult, 1, pp.395~402 (1986)
  5. 이상원, "젖산균과 청국균으로 발효시킨 용안육의 항산화, 항암효과 및 일산화질소생성", Food Science and Preservation, 14(5), pp.531~537 (2007), 한국식품저장유통학회
  6. 김미정, "김치 젖산균의 in vitro에서 콜레스테롤 저하 효과", Culinary Science & Hospitality Research, 12(4), pp.259~268 (2006), (사)한국조리학회
  7. 이하연, "추출방법에 따른 용아초의 육가공 식품 변패 젖산균에 대한 생육저해 효과", 산업식품공학, 16(2), pp.180~184 (2012), 한국산업식품공학회
  8. 박보연, "Evaluation of ginsenoside bioconversion of lactic acid bacteria isolated from kimchi", Journal of Ginseng Research, 41(4), pp.524~530 (2017), 고려인삼학회[DOI]
  9. 박은진, "팽이버섯(Flammulina velutipes) 분말의 젖산발효를 통한 고농도 γ-aminobutyric acid 함유 천연 발효조미료 개발", Food Science and Preservation, 24(2), pp.237~245 (2017), 한국식품저장유통학회
  10. 진효상, "김치 미생물 조성을 바탕으로 한 김치 스타터의 선정 및 효과", 한국식품영양과학회지, 37(5), pp.671~675 (2008), 한국식품영양과학회
  11. Jung BH, "Research status of biotransformation technology and industrial utilization technology", Korean Soc. Microbiol. Biotechnol, 8, pp.4057~4067 (1995)
  12. 이진희, "Safety Evaluation of Filamentous Fungi Isolated from Industrial Doenjang Koji", Journal of Microbiology and Biotechnology, 24(10), pp.1397~1404 (2014), 한국미생물·생명공학회[DOI]
  13. 조미나, "Bioconversion of Ginsenoside Rb1 to the Pharmaceutical Ginsenoside Compound K using Aspergillus usamii KCTC 6954", 한국미생물·생명공학회지, 42(4), pp.347~353 (2014), 한국미생물·생명공학회[DOI]
  14. Han MH, "Studies on hemicellulase system in Aspergillus niger", Korean J. Appl. Microbiol. Bioeng, 11, pp.193~199 (1983)
  15. 장미희, "김치에서 분리된 Leuconostoc 속 젖산균의 β-1,4-xylosidase 효소생산 특성", 한국식품과학회지, 43(2), pp.169~175 (2011), 한국식품과학회
  16. 최찬영, "Increase of Epigallocatechin in Green Tea Extract by Lactic Acid Bacteria Fermentation", 한국미생물·생명공학회지, 44(1), pp.62~67 (2016), 한국미생물·생명공학회[DOI]
  17. Marcolongo L, "The effect of Pleurotus ostreatus arabinofuranosidase and its evolved variant in lignocellulosic biomasses conversion", Fungal Genet. Biol, 72, pp.162~167 (2014)
  18. 장미희, "김치에서 분리된 젖산균의 β-glucosidase 활성 탐색", 산업식품공학, 14(3), pp.243~248 (2010), 한국산업식품공학회
  19. 오은희, "β-Glucuronidase 저해 활성이 우수한 결명자를 첨가한 상황 균사체 배양액의 생리활성", 한국식품영양학회지, 25(3), pp.620~628 (2012), 한국식품영양학회
  20. Lee KE, "Effect of kimchi intake on the composition of human large intestinal bacteria", Korean J. Food Sci. Technol, 28, pp.981~986 (1996)
  21. Nanno M, "Mutagenic activation of biliary metabolites of benzo(a)pyrene by β-glucuronidase-positive bacteria in human faeces", J. Med. Microbiol, 22, pp.351~355 (1986)
  22. 명대성, "대장암에 대한 장내 미생물 무리의 영향과 프로바이오틱스", 대한소화기학회지, 60(5), pp.275~284 (2012), 대한소화기학회
  23. Tranoy-Opalinski I, "β-Glucuronidase-responsive prodrugs for selective cancer chemotherapy: an update", Eur. J. Med. Chem, 74, pp.302~313 (2014)
  24. Kuroyama H, "Purification and characterization of a β-glucuronidase from Aspergillus niger", Carbohydr. Res, 333, pp.27~39 (2001)
  25. Russell WM, "Identification and cloning of gusA, encoding a new β-glucuronidase from Lactobacillus gasseri ADH", Appl. Environ. Microbiol, 67, pp.1253~1261 (2001)
  26. Tryland I, "Enzyme characteristics of β-D-galactosidase-and β-D-glucuronidase-positive bacteria and their interference in rapid methods for detection of waterborne coliforms and Escherichia coli", Appl. Environ. Microbiol, 64, pp.1018~1023 (1998)
  27. Rhee YK, "Inhibitory effect of Zizyphi frucus on β-glucuronidase and tryptophanase of human intestinal bacteria", Korean J. Food Sci. Technol, 30, pp.199~205 (1998)
  28. Falkenbach A, "Cyclosporin treatment in rheumatoid arthritis is associated with an increased serum activity of β-glucuronidase", Scand. J. Rheumatol, 22, pp.83~85 (1993)
  29. Baharudin MS, "Synthesis of indole analogs as potent β-glucuronidase inhibitors", Bioorg. Chem, 72, pp.323~332 (2017)
  30. Jeffrey SC, "Development and properties of β-glucuronide linkers for monoclonal antibody-drug conjugates", Bioconjug. Chem, 17, pp.831~840 (2006)
  31. 김현성, "Cloning and Expression of β-Glucuronidase from Lactobacillus brevis in E. coli and Application in Bioconversion of Baicalin and Wogonoside", Journal of Microbiology and Biotechnology, 19(12), pp.1650~1655 (2009), 한국미생물·생명공학회
  32. Callanan MJ, "Modification of Lactobacillus β-glucuronidase activity by random mutagenesis", Gene, 389, pp.122~127 (2007)
  33. Konishi T, "Properties of family 79 β-glucuronidases that hydrolyze β-glucuronosyl and 4-O-methyl-β-glucuronosyl residues of arabinogalactan-protein", Carbohydr. Res, 343, pp.1191~1201 (2008)
  34. Krahulec J, "Characterization of the new β-glucuronidase from Streptococcus equi subsp. zooepidemicus", Appl. Microbiol. Biotechnol, 74, pp.1016~1022 (2007)
  35. Kim DH, "Purification and characterization of β-glucuronidase from Escherichia coli HGU-3, a human intestinal bacterium", Biol. Pharm. Bull, 18, pp.1184~1188 (1995)
  36. Xu Y, "Sequence editing strategy for improving performance of β-glucuronidase from Aspergillus terreus", Chem. Eng. Sci, 167, pp.145~153 (2017)

관련 논문AI추천

이 간행물의 다른 논문 (18건)