신규 분리된 담수미세조류 Parachlorella sp.의 지방산 생산성 향상을 위한 배지 조성 연구
Investigation on Media Composition for Cultivation of a Newly Isolated Freshwater Microalga Parachlorella sp. to Enhance Fatty Acid Productivity
2
조회
0
다운로드
2
인용
0
참고문헌
키워드
초록
본 연구에서는 국내 낙동강 수계에서 신규하게 분리된 미세조류인 Parachlorella sp. 종의 바이오매스 및 지방산 생산성에 대한 배지의 영향을 연구하였다. 미세조류 배양에 통상적으로 사용되는 BG-11, TAP, BBM 배지를 사용하여 바이오매스 생산성은 TAP 배지에서, 지방산 축적은 BBM 배지에서 가장 잘 일어나는 것으로 확인되었고, 지방산 생산성을 향상시키기 위해 암모니아와 아세트산을 사용하는 TAP 배지의 조성을 변화하여 BBM 배지처럼 지방산 축적을 유도하며 바이오매스 생산성을 증가시킨 MTAP 배지를 개발하였다. 전체적인 바이오매스와 지방산 생산성을 높이기 위해서는 MTAP-1 배지가 적합하여 바이오매스 생산성과 지방산 생산성은 기존의 TAP 배지 대비 각각 14%, 45% 증가하였다. 생리 활성 효과로 인해 관심도가 높은 오메가-3 지방산의 생산에는 MTAP-4 배지가 가장 적합하여 바이오매스 생산성과 오메가-3 지방산 생산성이 기존 BBM 배지 대비 각각 18%, 39% 증가하여 목표 중점 생산물질(바이오매스, 총 지방산, 또는 오메가-3 지방산)의 생산성을 향상시킬수 있는 신규 배지 2종의 조성을 개발하였다.
Abstract
Parachlorella sp. is an efficient fatty acid producer that can be used in the production of biofuels, feeds, and fertilizers. Microalgae show varying responses to culture conditions, even those within the same species. In this study, growth and fatty acid composition of a newly isolated Parachlorella sp. from the Nakdong river of Korea in different culture media were investigated. The microalga was cultivated in 400 ml bubble column photobioreactors using BG-11, BBM, TAP, and modified TAP (MTAP) media. It was shown that using BBM led to greater fatty acid accumulation (34%), while using TAP medium led to greater biomass productivity (0.34 g/l/day). Composition of the TAP medium was modified to have the N:P ratio of BBM while also varying concentrations of N and P to improve fatty acid productivity. One of the modified TAP media, MTAP-1 (104.8 mgN/l, 135.2 mgP/l, N:P ratio = 0.77), showed the highest fatty acid concentration of 0.69 ± 0.04 g/l, while those from TAP and BBM were 0.48 ± 0.06 g/l and 0.40 ± 0.02 g/l, respectively. The results showed that microalgal fatty acid productivity could be enhanced by changing the N:P ratio and concentrations.
참고문헌 (29)
- Park H, "Theoretical calculations on the feasibility of microalgal biofuels : utilization of marine resources could help realizing the potential of microalgae", Biotechnol. J, 11, pp.1461~1470 (2016)
- 강시온, "미세조류 옥외 배양시스템을 이용한 바이오디젤 생산 및 도시하수 영양 염류 제거", 한국미생물·생명공학회지, 41(2), pp.207~214 (2013), 한국미생물·생명공학회[DOI]
- Kassim MA, "Towards biorefinery production of microalgal biofuels and bioproducts: Production of acetic acid from the fermentation of Chlorella sp. and Tetraselmis suecica hydrolysates", Green Sust. Chem, 7, pp.152~171 (2017)
- 조민호, "Optimization of Phototrophic Growth and Lipid Production of a Newly Isolated Microalga, Desmodesmus sp. KAERI-NJ5", 한국미생물·생명공학회지, 46(4), pp.377~389 (2018), 한국미생물·생명공학회
- Majid Mahdieh, "Characterization of the Growth, Total Lipid and Fatty Acid Profiles in Microalga, Nannochloropsis oceanica under Different Nitrogen Sources", 한국미생물·생명공학회지, 47(1), pp.11~19 (2019), 한국미생물·생명공학회[DOI]
- 박한울, "저온 생장성이 우수한 분리 미세조류 Tetraselmis sp. 5개주의 생장 패턴 및 지방산 조성 분석", 한국해양바이오학회지, 11(1), pp.23~28 (2019), (사)한국해양바이오학회[DOI]
- Baer S, "Optimization of spectral light quality for growth and product formation in different microalgae using a continuous photobioreactor", Algal Res, 14, pp.109~115 (2016)
- Shin DW, "Isolation of new microalga, Tetraselmis sp. KCTC12236BP, and biodiesel production using its biomass", J. Mar. Biosci. Biotechnol, 8, pp.39~44 (2016)
- 홍성주, "Enhanced Production of Fatty Acids in Three Strains of Microalgae using a Combination of Nitrogen Starvation and Chemical Inhibitors of Carbohydrate Synthesis", Biotechnology and Bioprocess Engineering, 22(1), pp.60~67 (2017), 한국생물공학회
- Recht L, "Patterns of carbohydrate and fatty acid changes under nitrogen starvation in the microalgae Haematococcus pluvialis and Nannochloropsis sp", App. Microbiol. Biotechnol, 94, pp.1495~1503 (2012)
- 김동건, "Transcriptomic Analysis of Haematococcus lacustris during Astaxanthin Accumulation under High Irradiance and Nutrient Starvation", Biotechnology and Bioprocess Engineering, 16(4), pp.698~705 (2011), 한국생물공학회
- 박승혜, "Comparison of the responses of two Dunaliella strains, Dunaliella salina CCAP 19", 18 and Dunaliella bardawil to light intensity with special emphasis on carotenogenesis, 2013, pp.28(2), 203~211[DOI]
- Lamers PP, "Carotenoid and fatty acid metabolism in nitrogen-starved Dunaliella salina, a unicellular green microalga", J. Biotechnol, 162, pp.21~27 (2012)
- Li X, "The microalga Parachlorella kessleri––A novel highly efficient lipid producer", Biotechnol. Bioeng, 110, pp.97~107 (2013)
- Heo J, "PhotoBiobox : A tablet sized, low-cost, high throughput photobioreactor for microalgal screening and culture optimization for growth, lipid content and CO2 sequestration", Biochem. Eng. J, 103, pp.193~197 (2015)
- 이승훈, "Increased Microalgae Growth and Nutrient Removal Using Balanced N:P Ratio in Wastewater", Journal of Microbiology and Biotechnology, 23(1), pp.92~98 (2013), 한국미생물·생명공학회[DOI]
- Karapinar Kapdan I, "Application of the Stover–Kincannon kinetic model to nitrogen removal by Chlorella vulgaris in a continuously operated immobilized photobioreactor system", J. Chem. Technol. Biot, 83, pp.998~1005 (2008)
- Mandalam RK, "Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures", Biotechnol. Bioeng, 59, pp.605~611 (1998)
- Zhu S, "Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalga Chlorella zofingiensis", Bioresour. Technol, 152, pp.292~298 (2014)
- Lee HS, "Specific light uptake rates can enhance astaxanthin productivity in Haematococcus lacustris", Bioprocess Biosyst. Eng, 39, pp.815~823 (2016)
- Sanz-Luque E, "Understanding nitrate assimilation and its regulation in microalgae", Front. Plant Sci, 6, pp.899~ (2015)
- Chalima A, "Utilization of volatile fatty acids from microalgae for the production of high added value compounds", Fermentation, 3, pp.54~ (2017)
- Bourre J, "Where to find omega-3 fatty acids and how feeding animals with diet enriched in omega-3 fatty acids to increase nutritional value of derived products for human : what is actually useful", J. Nutr. Health. Aging, 9, pp.232~242 (2005)
- omega-3 essential fatty acid ratio: The scientific evidence, "Omega-6", pp.2003, Simopoulos AP
- Ponnampalam E, "Effect of feeding systems on omega-3 fatty acids, conjugated linoleic acid and trans fatty acids in Australian beef cuts : potential impact on humnan health", Asia Pac. J. Clin. Nutr, 15, pp.21~29 (2006)
- Sayanova O, "Modulation of lipid biosynthesis by stress in diatoms", Philos. Trans. R. Soc. B. Biol. Sci, 372, pp.20160407~ (2017)
- Pereira SL, "Recent advances in the study of fatty acid desaturases from animals and lower eukaryotes", Prostaglandins Leukot. Essent. Fatty Acids, 68, pp.97~106 (2003)
- Sasaki M, "Coproduction of lipids and extracellular polysaccharides from the novel green alga Parachlorella sp. BX1. 5 depending on cultivation conditions", Biotechnol. Rept, 25, pp.e00392~ (2020)
- Taleb A, "Investigation of lipid production by nitrogen-starved Parachlorella kessleri under continuous illumination and day", night cycles for biodiesel application, 2018, pp.30, 761~772