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폐수를 이용한 겨울철 경제적 미세조류배양 시스템의 개발

Development of Economic Culture System Using Wastewater for Microalgae in Winter Season

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초록

The outdoor mass cultivation is not possible for microalgae in Korea all year round,due to cold winter season. It is not easy to maintain proper level of productivity of microalgae evenin winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were coveredadditionally, inside the original plastic layer of the greenhouse. The middle layer was made up ofplastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructedto get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triplycovered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivationof microalgae to minimize the production cost. Water temperature in the bioreactors remainedabove 10°C on average, even without any external heating system, proving that the triple-layeredgreenhouse is effective in keeping heat. Algal biomass reached to 0.37g L-1 with the highest temperature,in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorusremoval rate of 92% and 99%, respectively. When fatty acid composition was analyzed usinggas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the allexperiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation,but rather increased. Although the algal biomass productivity was not comparable to warmseasons, it was possible to maintain water temperature for algae cultivation even in the coldestseason, at the minimum cost.

Abstract

The outdoor mass cultivation is not possible for microalgae in Korea all year round,due to cold winter season. It is not easy to maintain proper level of productivity of microalgae evenin winter. To prevent a drastic decrease of temperature in a greenhouse, two layers were coveredadditionally, inside the original plastic layer of the greenhouse. The middle layer was made up ofplastic and the inner layer, of non-woven fabric. Acrylic transparent bioreactors were constructedto get more sunlight, not only from the upper side but also from the lateral and bottom directions. In winter at freezing temperatures, six different culture conditions were compared in the triplycovered, insulated greenhouse. Wastewater after anaerobic digestion was used for the cultivationof microalgae to minimize the production cost. Water temperature in the bioreactors remainedabove 10°C on average, even without any external heating system, proving that the triple-layeredgreenhouse is effective in keeping heat. Algal biomass reached to 0.37g L-1 with the highest temperature,in the experimental group of light-reflection board at the bottom, with nitrogen and phosphorusremoval rate of 92% and 99%, respectively. When fatty acid composition was analyzed usinggas-chromatography, linoleate (C18 : 3n3) occupied the highest proportion up to 61%, in the allexperiment groups. Chemical oxygen demand (COD), however, did not decrease during the cultivation,but rather increased. Although the algal biomass productivity was not comparable to warmseasons, it was possible to maintain water temperature for algae cultivation even in the coldestseason, at the minimum cost.

참고문헌 (19)

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