Ethanolic Extract of Pancake Mixture Powder Supplemented with Helianthus tuberosus Enhances Antidiabetic Effects via Inhibiting Inflammatory Mediator NO Production
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.
Abstract
Helianthus tuberosus is perennial plant as Compositae family and is shown various physiological activities such as analgesic, antipyretic, anti-inflammatory, anti-fungal, anti-spasmodic, aperient, cholagogue, diuretic, spermatogenic, stomachic, and tonic effects. In this study, we investigated the antidiabetic and anti-inflammatory effects of pancake mixture powder (PM) supplemented with H. tuberosus (PMH) in rat skeletal muscle L6 cells and murine macrophage RAW 264.7 cells, respectively. PM and PMH inhibited in vitro α-glucosidase activity. Glucose consumption was increased by PM and PMH without cytotoxicity in rat myoblast L6 cells. Western blot analysis revealed that PM and PMH down-regulated glycogen synthase kinase (GSK)-3β activation in L6 cells. PM and PMH inhibited inflammatory mediator, nitric oxide (NO) production without cytotoxicity in LPS-induced RAW 264.7 cells. The anti-diabetic and anti-inflammatory effects of PMH was more stronger than those of PM. Anti-diabetic and anti-inflammatory effects of PMH would be due to functional characteristics of the supplemented H. tuberosus and the presence of garlic and onion used as ingredients of PM. Taken together, our results that addition of functional materials such as H. tuberosus in product has synergic effects and PMH is potential candidate for treatment of diabetes through inhibiting inflammation.
참고문헌 (47)
- Chen F, "Analysis of phenolic acids of jerusalem artichoke (Helianthus Tuberosus L.) responding to salt-stress by liquid chromatography", tandem mass spectrometry, 2014, pp.2014, 568043~
- Chen F, "Phenolics and antifungal activities analysis in industrial crop jerusalem artichoke (Helianthus Tuberosus L.) leaves", Ind. Crops Prod, 47, pp.339~345 (2013)
- Chen F, "Evaluation of antifungal phenolics from Helianthus tuberosus L. leaves against Phytophythora capsici Leonian by chemometric analysis", Molecules, 24, pp.4300~ (2019)
- Gibson GR, "Dietary modulation of the human colonic microbiota : introducing the concept of prebiotics", J. Nutr, 125, pp.1401~1412 (1995)
- Jung YJ, "Jerusalem Artichoke(Helianthus tuberosus)on the inflammatory parkcrine loop between macrophage and adipocytes", J. Agric. Food. Chem, 64, pp.9317~9325 (2016)
- Wang PC, "Anti-diabetic polysaccharides from natural sources : A review", Carbohydr. Polym, 148, pp.86~97 (2016)
- Wang Y, "Nutritional value, bioactivity and application potential of Jerusalem artichoke (Helianthus tuberosus L.) as a neotype feed resource", Anim. Nutr, 6, pp.429~437 (2020)
- Muscogiuri G, "Nutritional guidelines for the management of insulin resistance", Crit. Rev. Food. Sci. Nutr, 2, pp.1~14 (2021)
- Ritz E, "Nephropathy in type 2 diabetes", J. Inter. Med, 245, pp.111~126 (1999)
- Vinik AI, "Diabetic neuropathies", Diabetologia, 43, pp.957~973 (2000)
- Kolb H, "An immune origin of type 2diabetes?", Diabetologia, 48, pp.1038~1050 (2005)
- Koenen M, "Obesity, adipose tissue and vascular dysfunction", Circ. Res, 128, pp.951~968 (2021)
- Maggio CA, "Obesity and type 2 diabetes", Endocrinol. Metab. Clin. North Am, 32, pp.805~822 (2003)
- Mariana M, "Genetic and environmental factors associated with type 2 diabetes and diabetic vascular complications", Rev. Diabet. Rev, 9, pp.6~22 (2012)
- Rask-Madsen C, "Vascular complications of diabetes : Mechanisms of injury and protective factors", Cell. Metab, 1, pp.20~33 (2013)
- Keen H, "Reducing the burden of diabetes : Managing cardiovascular disease", Diabetes Metab. Res. Rev, 15, pp.186~196 (1999)
- Kuzuya T, "Report of the committee on the classification and diagnostic criteria of diabetes mellitus", Diabet. Res. Clin. Pract, 55, pp.65~85 (2002)
- Manson JE, "A prospective study of maturity-onset diabetes mellitus and risk of coronary heart disease and stroke in women", Arch. Inter. Med, 151, pp.1141~1147 (1991)
- Pickup JC, "Is type II diabetes mellitus a disease of the innate immune system?", Diabetologia, 41, pp.1241~1248 (1998)
- Kolb H, "An immune origin of type 2diabetes?", Diabetologia, 48, pp.1038~1050 (2005)
- Ferrero-Miliani L, "Chronic inflammation : importance of NOD2 and NALP3 in interleukin-1 β generation", Clin. Exp. Immunol, 147, pp.227~235 (2007)
- Choy EH, "Cytokine pathways and joint inflammation in rheumatoid arthritis", N. Engl. J. Med, 344, pp.907~916 (2001)
- Hamza N, "Prevention of type 2 diabetes induced by high fat diet in the C57BL", 6J mouse by two medicinal plants used in traditional treatment of diabetes in the east of Algeria, 2010, pp.128, 513~518
- Ibeh BO, "Preliminary study of antidiabetic activity of the methanolic leaf extract of Axonopus compressus (P. Beauv) in alloxan-induced diabetic rats", J. Ethnopharmacol, 3, pp.713~716 (2011)
- Ferris FLD, "Diabetic retinopathy", Diabet. Care, 16, pp.322~325 (1993)
- Li T, "A microplate-based screening method for α-glucosidase inhibitors", Chin. J. Clin. Pharmacol. Ther, 10, pp.1128~1134 (2005)
- Estrada DE, "Stimulation of glucose uptake by the natural coenzyme alpha-lipoic acid", thioctic acid : Participation of elements of the insulin signaling pathway, 1996, pp.45, 1798~1804
- Yin J, "Shilianhua extract inhibits GSK-3 and promotes glucose metabolism", Am. J. Physiol. Endocrinol. Metab, 296, pp.E1275~E1280 (2009)
- Zhang S, "Antioxidant and antidiabetic activities of vanadium-binding protein and trifuhalol A", J. Food Biochem, 44, pp.e13540~ (2020)
- 정현주, "열처리에 따른 돼지감자 Methanol 추출물의 항산화 및 α-glucosidase 저해 효과", 한국약용작물학회지, 19(4), pp.257~263 (2011), 한국약용작물학회
- Melmer A, "The role of physical exercise in obesity and diabetes", Praxis, 107, pp.971~976 (2018)
- Pulgaron ER, "Obesity and type 2 diabetes in children : epidemiology and treatment", Curr. Diab. Rep, 14, pp.508~ (2014)
- Sawicka B, "Jerusalem artichoke (Helianthus tuberosus L.) as a medicinal plant and its natural products", Cell. Mol. Biol, 66, pp.160~777 (2020)
- Defrono RA, "Skeletal muscle insulin resistance is the primary defect in type-2 diabetes", Diabet. Care, 32, pp.S157~S163 (2009)
- Cantley LC, "The phosphoinositide 3-kinase pathway", Science, 296, pp.1655~1657 (2002)
- Xu P, "Piperlongumine attenuates oxidative stress, inflammatory, and apoptosis through modulating the GLUT-2", 4 and AKT signaling pathway in streptozotocin-induced diabetic rats, 2021, pp.35, 1~12
- Postic C, "Evidence for a transient inhibitory effect of insulin on GLUT2 expression in the liver : studies in vivo and in vitro", Biochem. J, 293, pp.119~124 (1993)
- Henriksen EJ, "Modulation of muscle insulin resistance by selective inhibition of GSK-3 in Zucker diabetic fatty rats", Am. J. Physiol. Endocrinol. Metab, 284, pp.E892~E900 (2003)
- Hoeflich KP, "Requirement for glycogen synthase kinase-3β in cell survival and NF-κB activation", Nature, 406, pp.86~90 (2000)
- Ilouz R, "Identification of novel glycogen synthase kinase-3β substrateinteracting residues suggests a common mechanism for substrate recognition", J. Biol. Chem, 281, pp.30621~30630 (2006)
- Chen ZJ, "Glycine improves ischemic stroke through miR-19a-3p", AMPK, HO-1 pathway, pp.Drug Des. Dev. Ther, 2020[DOI]
- Cross DA, "The inhibition of glycogen synthase kinase-3 by insulin or insulin-like growth factor 1 in the rat skeletal muscle cell line L6 is blocked by wortmannin, but not by rapamycin : evidence that wortmannin blocks activation of the mitogen-activated protein kinase pathway in L6 cells between Ras and Raf", Biochem. J, 303, pp.21~26 (1994)
- Martin M, "Toll-like receptor mediated cytokine production is differentially regulated by glycogen synthase kinase 3", Nat. Immunol, 6, pp.777~784 (2005)
- Shoelson SE, "Inflammation and insulin resistance", J. Clin. Investig, 116, pp.1793~1801 (2006)
- Gautam S, "Ethanolic extract of Allium cepa stimulates glucose transporter typ 4-mediated glucose uptake by the activation of insulin signaling", Planta Med, 81, pp.208~214 (2015)
- Motloung DM, "Synthesis, characterization, antidiabetic and antioxidative evaluation of a novel Zn(II)-gallic acid complex with multi-facet activity", J. Pharm. Pharmacol, 72, pp.1412~1426 (2020)
- Noipha K, "Enhancement of glucose transport by selected plant foods in muscle cell line L6", Diabetes Res. Clin. Pract, 89, pp.22~26 (2010)