Synbiotics 첨가 사료 투여가 넙치(Paralichthys olivaceus)의 성장과 비특이적 면역 반응 및 세균성 감염 폐사에 미치는 영향
Effect of Synbiotics-Added Feeds on the Growth, Non-Specific Immune Response, and Disease Resistance of Olive Flounders, Paralichthys olivaceus
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
본 연구는 기존의 probiotics로서의 안전성을 입증한 B. cabrialesii sp. K1과 prebiotics 중 inulin 3%와 혼합하여synbiotics로서의 넙치의 성장과 비특이적 면역 반응 및 세균성 감염에 따른 폐사에 미치는 영향을 검토하였다. 8주 동안 단일 프로바이오틱스 B. cabrialesii sp. K1과 inulin 3% 를 혼합한 신바이오틱스를 급이 한 결과 성장에서는 유의적인 차이를 보이지 않았다. 혈액학적 검사의 경우 AST, total protein 및 cholesterol에서 유의적인 차이를 보였고 ALT와glucose는 유의적인 차이가 없었다. 비특이적 면역반응에서는 유산균 첨가 또는 주사제로 인한 라이소자임 활성이 유익하다고 알려져 있으나 본 연구에서는 8주동안 대조구와 비교시 유의적 차이가 없었다. 또한 B. cabrialesii K1의 경우 단일 프로바이오틱스구보다 신바이오틱스구가 대조구에 비해NBT 활성이 증가된 것을 확인할 수 있었다. 다음 넙치의 사이토카인 발현을 확인한 결과 B. cabrialesii sp. K1를 단일(probiotics구)로 사용했을 경우 대조구와 비교 시 비장에서유의적으로 낮게 발현되었으나 synbiotics구의 경우 비장에서 발현이 유의하게 증가되었다. 그러나 나머지 간과 장, 신장에서는 세 개의 그룹 간의 유의적 차이를 나타내지 않았다. 마지막으로 2주동안의 인위 감염을 검토한 결과 대조구는 E. tarda 95%, S. parauberis 85%, S. iniae 85%로 각각의 폐사율을 나타냈다. Probiotics구는 E. tarda 85%, S. parauberis 80%, S. iniae 80%의 폐사율을 보였고, synbiotics 구는 E. tarda 80%, S. parauberis 80%, S. iniae 85%로 각각 나타났다.
Abstract
In this study, the effect of a synbiotic mixture of probiotics and 3% inulin on the growth, non-specific immune response, and mortality due to disease resistance of Paralichthys olivaceus was investigated. The results revealed that an 8-week diet of synbiotic-added feed, had no significant effect on the growth of P. olivaceus, Analysis of the hematological parameters revealed that there was a significant difference in the content of AST, total protein, and cholesterol among the groups, whereas there was no significant difference in the ALT and glucose contents. Further, there was no significant difference in the lysozyme activity of the synbiotic and control group after 8 weeks' feeding. In addition, when the symbiotic mixture was used rather than probiotics alone, the NBT activity of the synbiotic group increased compared to that of the control group. Moreover, when only probiotics were used, the cytokine expression in the spleen of the samples was significantly lower than that in the control group, whereas the expression was significantly higher in the spleen of the synbiotics group. However, there was no significant difference in the cytokine expression in the liver, intestine, and kidney of the three groups. Lastly, after injection for 2 weeks, the mortality rates of the control group towards Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae were 95, 85 and 85%, respectively; those of the probiotics group were 85, 80, and 80%, respectively; and those of the synbiotics group were 80, 80, and 85%, respectively.
참고문헌 (35)
- The state of world fisheries and aquaculture 2020. Sustainability in action, "FAO", ~ (2020)
- 2021 COFI declaration for sustainable fisheries and aquaculture, "FAO", ~ (2021)
- 조미영, "2005년부터 2006년 사이 우리나라 양식 넙치, Paralichthys olivaceus의 세균성 질병에 대한 역학 조사", 한국어병학회지, 20(1), pp.61~70 (2007), 한국어병학회
- Takano T, "The efficacy of five avirulent Edwardsiella tarda strains in a live vaccine against Edwardsiellosis in Japanese flounder, Paralichthys olivaceus", Fish Shellfish Immunol, 29, pp.687~693 (2010)
- Liu F, "Comparative study of the vaccine potential of six outer membrane proteins of Edwardsiella tarda and the immune responses of flounder(Paralichthys olivaceus)after vaccination", Vet. Immunol. Immunopathol, 185, pp.38~47 (2017)
- Li J, "Generation and evaluation of virulence attenuated mutants of Edwardsiella tarda as vaccine candidates to combat edwardsiellosis in flounder(Paralichthys olivacues)", Fish Shellfish Immunol, 43, pp.175~180 (2015)
- 심재동, "한국 양식 넙치 폐사피해 모니터링", 한국어병학회지, 32(1), pp.29~35 (2019), 한국어병학회[DOI]
- 김경욱, "제주지역 넙치(Paralichthys olivaceus) 연쇄구균병 원인체의 분리특성과 Streptococcus parauberis의 혈청형 변화", 한국어병학회지, 33(2), pp.119~125 (2020), 한국어병학회
- Inglis V, , Proceedings of the meeting on the use of chemicals in aquaculture in Asia, pp.7~22 (1996)
- 장익수, "Probiotics를 양식넙치에 투여시 Streptococcus iniae에 대한 면역반응 및 병저항성", 미생물학회지, 49(2), pp.172~178 (2013), 한국미생물학회[DOI]
- Kim J, "Oral administration effect of yacon, ginger and blueberry on the growth, body composition and plasma chemistry of juvenile olive flounder (Paralichthys olivaceus) and immunity test against Streptococcus iniae compared to a commercial probiotc, Lacto bacillus fermentum", Aquac. Rep, 15, pp.100~212 (2019)
- Pandy KR, "Probiotics, prebiotics and synbiotics-a review", J. Food Sci. Technol, 52, pp.7577~7587 (2015)
- 문채윤, "한국전통발효식품에서 분리한 Probiotics의 특징 및 Synbiotics 항균활성 효과", 한국미생물·생명공학회지, 49(4), pp.552~558 (2021), 한국미생물·생명공학회[DOI]
- Anderson DP, "Duration of protection against Aeromonas salmonicida in brook trout immunostimulated with glucan or chitosan by injection or immersion", Progressive Fish-Culturist, 56, pp.258~261 (1994)
- Ellis AE, "Techniques in Fish Immunology", pp.101~103 (1990), SOS publications
- Li Y, "Effects of dietary supplementation of Lactobacillus plantarum and Bacillus subtilis on growth performance, survival, immune response, antioxidant capacity and digestive enzyme activity in olive flounder", Aquac. Fish, 6, pp.283~288 (2021)
- Alvarellos SS, "Time course of osmoregulatory and metabolic changes during osmotic acclimation in Sparus auratus", J. Exp. Biol, 208, pp.4291~4304 (2005)
- 박인석, "Hematological Responses, Survival, and Respiratory Exchange in the Olive Flounder, Paralichthys olivaceus, during Starvation", Animal Bioscience, 25(9), pp.1276~1284 (2012), 아세아·태평양축산학회[DOI]
- 조성환, "Effect of Fasting and Refeeding on Growth and Blood Chemistry in Juvenile Olive Flounder Paralichthys olivaceus L.", 한국양식학회지, 22(1), pp.11~15 (2009), 한국양식학회
- 박상현, "생균제 첨가사료가 조피볼락 Sebastes schlegeli의 성장 및혈액성분에 미치는 영향", 한국양식학회지, 21(1), pp.1~6 (2008), 한국양식학회
- Khaled HB, "Effect of protein hydrolysates from sardinelle(Sardinella aurita)on the oxidative status and blood lipid profile of cholesterol-fed rats", Food Res. Int, 45, pp.60~68 (2012)
- Walker DK, "Relationships among bile tolerance, bile salt deconjugation, and assimilataion of cholesterol by lactobacillus acidophilus", J. Dairy Sci, 76, pp.956~961 (1993)
- Klaver, "The assumed assimilation of cholesterol by Lactobacilli and Bifidobacterium bifidum is due to their bile salt-deconjugationg activity", Appl. Environ. Microbiol, 59, pp.1120~1124 (1993)
- 정승희, "생약재 첨가 사료를 투여한 넙치(Paralichthys olivaceus)의 비특이적 면역반응, 혈액성분 및 항병력 효과", 한국어병학회지, 15(1), pp.25~35 (2002), 한국어병학회
- Kim, Yu-Ri, "Effect of a Probiotic Strain, Enterococcus faecium, on the Immune Responses of Olive Flounder (Paralichthys olivaceus)", Journal of Microbiology and Biotechnology, 22(4), pp.526~529 (2012), 한국미생물·생명공학회[DOI]
- Taoka Y, "Use of live and dead probiotic cells in tilapia Oreochromis niloticus", Fish. Sci, 72, pp.755~766 (2006)
- Sharifuzzaman SM, "Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout", Fish Shellfish Immunol, 27, pp.440~445 (2009)
- Harikrishnan R, "Immunomodulatory effect of probiotics enriched diets on Uronema marinum infected live flounder", Fish Shellifh Immunol, 30, pp.964~971 (2011)
- Sorimachi K, "Secretion of TNF-α, IL-8 and nitric oxide by macrophages activated with polyanions, and involvement of interferon-γ in the regulation of cytokine secretion", Cell Struct. Funct, 26, pp.103~108 (1999)
- Standen BT, "Probiotic Pediococcus acidilactici modulates both localized intestinal-and peripheral-immunity in tilapia(Oreochromis niloticus)", Fish Shellfish Immunol, 35, pp.1097~1104 (2013)
- Alejo A, "Chemokines in teleost fish species", Dev. Comp. Immunol, 35, pp.1215~1222 (2011)
- Scheller J, "The pro-and antiinflammatory properties of the cytokine interleukin-6", Biochim. Biophys. Acto-Mol. Cell Res, 1813, pp.878~888 (2011)
- Jang WJ, "Effect of dietary differences on changes of intestinal microbiota and immune-related gene expression in juvenile olive flounder(Paralichthys olivaceus)", Aquaculture, 527, pp.735442~ (2020)
- Cha JH, "Evaluations of Bacillus spp. As dietary additives on growth performance, innate immunity and disease resistance of live flounder (Paralichthys olivaceus) against Streptococcus iniae and as water additives", Aquaculture, 402-403, pp.50~57 (2013)
- Kim D, "Lactococcus lactis BFE920 activates the innate immune system of olive flounder(Paralichthys olivaceus), resulting in protection against Streptococcus iniae infection and enhancing feed efficiency and weight gain in large-scale field studies", Fish Shellifh Immunol, 35, pp.1585~1590 (2013)