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유산균을 활용한 미역발효추출물이 3T3-L1에서 지방세포 분화에 미치는 영향

Effects of Undaria pinnatifida and Leuconostoc mesenteroides Fermented Extracts on Adipocyte Differentiation in 3T3-L1 Cells

Moon Min Woo|Ra Chae Hun
한국미생물·생명공학회 (The Korean Society For Microbiology And Biotechnology)|2024.09|한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)|제52권 제3호|pp. 298-303|DOI 10.48022/mbl.2405.05002

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키워드

ObesityfermentationUndaria pinnatifidaLeuconostoc mesenteroidesadipogenesis
주제분류생물학

초록

유산균 L. mesenteroides SGL152를 이용한 미역발효 추출물로부터 3T3-L1 지방세포 분화에 미치는 영향을 살펴보기 위해 MTT assay, Oil Red O staining, RT-qPCR 실험을 진행하였다. 유산균의 미역발효 추출물(UFM)의 농도별세포 독성 실험 결과, 200 μg/ml 농도 이하에서는 비세포독성으로 판단된다. 또한 3T3-L1 지방세포 분화와 동시에 추출물을 농도별로 처리하여 분화를 진행한 결과, 대조군과 비교하여 유의적으로 지방구 형성 및 지방분화를 억제하였다. 이러한 연구결과는 유산균을 이용한 해조류 발효과정 및 복합추출물에 있어서 유용한 정보를 제공할 것으로 판단된다.

Abstract

This study aimed to examine the potential inhibitory effect of Undaria pinnatfida fermented by Leuconostoc mesenteroides (UFM) on preadipocyte differentiation in the 3T3-L1 cell line. Ethanol extracts from UFM were prepared and tested for cell viability, Oil Red O staining, and quantitative reverse transcription-PCR (RT-qPCR) in 3T3-L1 adipocytes. Treatment of preadipocytes with UFM at different concentrations (50, 100, and 200 μg/ml) found that it inhibited lipid accumulation by 66.80%, 61.59%, and 55.94%, respectively. Furthermore, RT-qPCR showed that UFM extract reduced the gene expressions of CCAAT/enhancer-binding protein α (C/EBP α), peroxisome proliferator-activated receptor γ (PPAR γ), adipocyte-specific lipid binding protein (aP2), fatty acid synthase (FAS), and ATP citrate lyase (ACL), respectively. These results suggest that UFM can be a beneficial functional ingredient to prevent obesity in the food industr

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