Pre-adaptation to Cu during Plant Tissue Culture Enhances Cu Tolerance and Accumulation in Begonia (Begonia evansiana Andr.)
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Abstract
A simple and eficient protocol was developed for culturingCu-tolerant and Cu-acumulating plantsvia pre-adaptation to Cu during plant isue culture. We ind uced multiple shots from begonia ( Begoniaevansiana Andr.) leaf explants on MS medium suplemented with naphtaieneacetic acid and benzyladenine.After 3 months, smal plantlets were transfered to MS medium suplemented with 10 . M CuCl 2 for pre-adap-tation to Cu and cultured for 5 months. Then, these plantletswere individualy planted in pots containing artificialsoil. An aditional 50 mg of Cu disolved in 1/4 strength MS s olution was aplied to each pot during irigationover the course of 2 months. We planted pre-adapted and control begonias in soil from the Il-Kwang Mine, anabandoned Cu mine in Pusan, Korea, to examine their ability to tolerate and acumulate Cu for phytore-mediation. Pre-adapted begonias acumulated 1,20 . g Cu/g dry rot isue over the course of 45 days. Onthe other hand, non-Cu-adapted controls acumulated only 85 . g Cu/g dry rot isue. To enhance Cuextraction, chelating agents, ethylenediamine tetracetic acid (EDTA)-dipotasiun and pyridine-2,6-dicarboxylicacid (PDA), were aplied. While the chelating agents did notenhance acumulation of Cu in the rots of controlbegonias, EDTA aplication increased the level of Cu in the rots of pre-adapted begonias twofold (to 2,50. g Cu/g dry rot isue). Because pre-adapted begonias acum ulated a large amount of Cu, mainly in theirrots, they could be used for phytostabilization of Cu-contaminated soils. In adition, as a flowering plant,begonias can be used to create aestheticaly pleasing remed iation sites.
참고문헌 (24)
- "Heavy metals in soils", pp.151~176 (1990), John Wiley and Sons Inc
- "Relationships between extractable copper soil properties and copper uptake by wild plants in vineyard soils", 102, pp.151~161 (1998)
- "Phytoremediation of soil metals", 8, pp.279~284 (1997)
- "Phytochelatins and methallothioneins:Roles in heavy metal detoxification and homeostasis", 53, pp.159~182 (2002)
- "Methods of soil analysis American Society of Agronomy", pp.545~567 (1965)
- "Role of uranium speciation in the uptake and translocation of uranium by plants", 49, pp.1183~1190 (1998)
- "Phytoextraction of zinc by Oat barley Hordeum vulgare and Indian mustard Brassica juncea", 32, pp.802~806 (1998)
- "Genetic improvement of heavy metal tolerance in plants by transfer of the yeast metallothionein gene", 196, pp.277~281 (1997)
- "Lead phytoextraction:species variation in lead uptake and translocation", 134, pp.75~84 (1996)
- "Phytoremediation:novel approaches to cleaning up polluted soils", 16, pp.133~141 (2005)
- "Accumulation of copper by roots cotyledons and leaves of sunflower", 86, pp.151~155 (2003)
- "A revised medium for rapid growth and bio-assays with tobacco tissue cultures", 15, pp.473~497 (1962)
- "Responses of maize plants to copper stress mineral content and ultrastructure of roots", 35, pp.167~176 (1995)
- "Acclimation of Salix to metal stress", 137, pp.303~314 (1997)
- "Plant genetic engineering may help with environmental cleanup", 93, pp.3164~3166 (1996)
- "Sources and forms of potentially toxic metals in soil- plant systems Toxic metals in soil-plant systems", pp.3~25 (1994)
- "Mercuric ion reduction and resistance in transgenic Arabidopsis thaliana plants expressing a modified bacterial merA gene", 93, pp.3182~3187 (1996)
- "Phytoremediation", 49, pp.643~668 (1998)
- "Ex situ remediation of a metal contaminated superfund soil using selective extractants", 124, pp.639~645 (1998)
- "Phytoremediation A novel and promising approach for environmental clean up", 24, pp.97~124 (2004)
- "The role of EDTA in lead transport and accumulation by Indian Mustard", 117, pp.447~453 (1998)
- "transport and release by wetland plants implication for phytoremediation and restoration", 30, pp.685~700 (2004)
- "Ecological restoration of mine degraded soils with emphasis on metal contaminated soils", 50, pp.775~780 (2003)
- "Received July 27 Accepted August 16" (2007)