Over-expression of Cu/ZnSOD Increases Cadmium Tolerance in Arabidopsis thaliana
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Abstract
Over-expresion of a coper/zinc superoxide dismutase (Cu /ZnSOD) resulted in substantialyincreased tolerance to cadmium exposure in Arabidopsis thaliana . Lower lipid peroxidation and H 2O2 acu-mulation and the higher activities of H2O2 scavenging enzymes, including catalase (CAT) and ascorbateperoxidase (APX) in transformants (CuZnSOD-tr) compared to untransformed controls (wt) indicated thatoxidative stres was the key factor in cadmium tolerance. Although progresive reductions in the dark-adaptedphotochemical eficiency (Fv/Fm) and quantum eficiency ield were observed with increasing cadmium levels,the chlorophyl fluorescence parameters were les marked in CuZnSOD-tr than in wt. These observationsindicate that oxidative stres in the photosynthetic aparatus is a principal cause of Cd-induced phytotoxicity,and that Cu/ZnSOD plays a critical role in protection against Cd-induced oxidative stres.
참고문헌 (51)
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- "Received July 9 Accepted August 2" (2007)
