In vitro Selection of Acifluorfen-tolerant Solanum ptycanthum and PhenotypicVariation inRegenerated Plants

Abstract

Aciflúorfen-tolerant callus lines of Solanum ptycanthem were isolated by stepwise selection. Growth of the unselected line was completely inhibited at 0.5 uM. while some selected lines grew at 8 uM acifluorfen. Twenty-two of twenty-five acifluorfen-tolerant callus lines regenerated shoots. Many of the regenerated somaclones were variants, differing in leaf shape, leaf color, number of flower parts, flower color, and fertility. The acifluorfen tolerant S. ptycanthum callus lines differed.