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High-Quality Whole Genome Sequence of a Linezolid-Resistant and Vancomycin-Susceptible Enterococcus faecalis Isolate ES-2-1 from a Pig Stool in South Korea

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초록

We report the whole genome sequence of a linezolid-resistant and vancomycin-susceptible Enterococcus faecalis strain, ES-2-1, which was isolated from a pig stool in South Korea. The assembled genome of ES-2-1 consists of a 2,648,168-bp circular chromosome containing the optrA gene (encoding the ABC-F type ribosomal protection protein), an 84,891-bp plasmid containing numerous antimicrobial resistance genes, and an 82,106-bp cryptic plasmid. The ES-2-1 strain belongs to sequence type 1024 (ST1024) and carries multidrug resistant genes including the optrA (oxazolidinone phenicol transferable resistance A) gene, which confers linezolid resistance.

Abstract

We report the whole genome sequence of a linezolid-resistant and vancomycin-susceptible Enterococcus faecalis strain, ES-2-1, which was isolated from a pig stool in South Korea. The assembled genome of ES-2-1 consists of a 2,648,168-bp circular chromosome containing the optrA gene (encoding the ABC-F type ribosomal protection protein), an 84,891-bp plasmid containing numerous antimicrobial resistance genes, and an 82,106-bp cryptic plasmid. The ES-2-1 strain belongs to sequence type 1024 (ST1024) and carries multidrug resistant genes including the optrA (oxazolidinone phenicol transferable resistance A) gene, which confers linezolid resistance.

참고문헌 (7)

  1. Meka VG, "Antimicrobial resistance to linezolid", Clin. Infect. Dis, 39, pp.1010~1015 (2004)
  2. "2019. Critically Important Antimicrobials for Human Medicine 6th Revision" (2018)[URL]
  3. Brenciani A, "Oxazolidinones : mechanisms of resistance and mobile genetic elements involved", J. Antimicrob. Chemother, 77, pp.2596~2621 (2022)
  4. Choi HJ, "Complete genome sequence of Salmonella enterica serotype Enteritidis MFDS1018147 isolated from braised burdock in Korea", Korean J. Microbiol, 59, pp.42~44 (2023)
  5. Clinical and Laboratory Standards Institute(CLSI), "Performance Standards for Antimicrobial Susceptibility Testing M10032th edition", ~ (2022)
  6. Prystowsky J, "Resistance to linezolid : characterization of mutations in rRNA and comparison of their occurrences in vancomycin-resistant enterococci", Antimicrob. Agents Chemother, 45, pp.2154~2156 (2001)
  7. Ni J, "Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses", Front. Sustain. Food Syst, 7, pp.1179078~ (2023)

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