Draft Genome Sequence and Antimicrobial Activity of Amycolatopsis japonica SB7-3 Isolated from Soil in Thailand
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This study explores genome analysis and antimicrobial activity of actinobacterium strain SB7-3, isolated from soil. Based on genome sequence analysis, strain SB7-3 was found to be closely related to Amycolatopsis japonica DSM 44213T. The draft genome of strain SB7-3 was 8463214 bp in 19 contigs with a G+C content of 69.10%. The average nucleotide identity-Blast (ANIb) and average nucleotide identity-MUMmer (ANIm) values of strain SB7-3 and A. japonica DSM 44213T were 96.18% and 96.93%, and the digital DNA-DNA hybridization (dDDH) values were 72.90%, respectively. Genomic analysis of strain SB7-3 indicated that the gene encodes enzymes and gene clusters associated with the biosynthesis of bioactive compounds. This strain demonstrated antimicrobial activity against Kocuria rhizophila ATCC 9341, Bacillus subtilis ATCC 6633, and Staphylococcus aureus ATCC 6538.
Abstract
This study explores genome analysis and antimicrobial activity of actinobacterium strain SB7-3, isolated from soil. Based on genome sequence analysis, strain SB7-3 was found to be closely related to Amycolatopsis japonica DSM 44213T. The draft genome of strain SB7-3 was 8463214 bp in 19 contigs with a G+C content of 69.10%. The average nucleotide identity-Blast (ANIb) and average nucleotide identity-MUMmer (ANIm) values of strain SB7-3 and A. japonica DSM 44213T were 96.18% and 96.93%, and the digital DNA-DNA hybridization (dDDH) values were 72.90%, respectively. Genomic analysis of strain SB7-3 indicated that the gene encodes enzymes and gene clusters associated with the biosynthesis of bioactive compounds. This strain demonstrated antimicrobial activity against Kocuria rhizophila ATCC 9341, Bacillus subtilis ATCC 6633, and Staphylococcus aureus ATCC 6538.