Safety Assessment of Leuconostoc Isolates from Kimchi, a Korean Traditional Fermented Vegetable
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초록
김치로부터 분리된 Leuconostoc carnosum 7 균주, Leu. lactis 2 균주, Leu. pseudomesenteroides 3 균주를 대상으로항생제 내성 및 용혈 활성을 평가하였다. 항생제 내성 평가는 8종의 항생제에 대해 수행하였으며, clindamycin, erythromycin, gentamicin, streptomcyin에 대해서는 모든균주가 감수성을 나타냈다. 반면, ampicillin, chloramphenicoal, kanamycin, tetracycline에 대해서는 각각 7개 균주(AK0M18, AK0M19, AK10T02, CK5T06, CK10T11, PK0M04, Y_C0M12), 2개 균주(PK0M04, Y_C0M12), 8개균주(AK0M18, AK0M19, AK10T02, CK5T02, CK5T06, P_C5T16, P_C5T19, Y_C0M12), 1개 균주(PK0M04)가 내성을 보였다. 항생제 대해 내성을 보인 균주에서 획득형 항 생제 내성 유전자의 존재 여부를 확인하기 위해 관련 유전자에 대해 PCR 증폭을 수행하였으나, 모든 균주에서 해당유전자는 증폭되지 않았다. 또한, 용혈 활성에 대해서는 α 및β 형 용혈 활성을 평가한 결과, 모든 균주가 용혈 활성을 보이지 않았다. 본 연구 결과는 실험에 사용된 균주들이 항생제 감수성에 있어 균주 특이적인 결과를 나타냄을 보여주며, 이는 산업적으로 종균을 사용하기전 균주별 항생제 감수성 평가가 필수적으로 이루어져야 함을 시사한다.
Abstract
The antibiotic resistance and hemolytic activity of 7 Leuconostoc carnosum strains, 2 Leu. lactis strains, and 3 Leu. pseudomesenteroides strains isolated from kimchi were evaluated. Antibiotic resistance was tested against 8 antibiotics, with all strains showing sensitivity to clindamycin, erythromycin, gentamicin, and streptomycin. However, 7 strains (AK0M18, AK0M19, AK10T02, CK5T06, CK10T11, PK0M04, and Y_C0M12), 2 strains (PK0M04 and Y_C0M12), 8 strains (AK0M18, AK0M19, AK10T02, CK5T02, CK5T06, P_C5T16, P_C5T19, and Y_C0M12), and 1 strain (PK0M04) exhibited resistance against ampicillin, chloramphenicol, kanamycin, and tetracycline, respectively. To determine if the resistant strains harbored acquired antibiotic resistance genes, PCR amplification was conducted, but no gene amplification was observed in any of the strains. Regarding hemolytic activity, α- and β-hemolysis were assessed, and none of the strains showed any hemolytic activity. While none of the strains exhibited hemolysis, strain-specific results were observed for antibiotic resistance. Through this study, it was confirmed that the antibiotic susceptibility of the strains tested in this experiment was strain-specific. This suggests that evaluating antibiotic susceptibility for each strain is necessary before using them as starter cultures in industrial applications.