Safety and Technological Characterization of Staphylococcus xylosus and Staphylococcus pseudoxylosus Isolates from Fermented Soybean Foods of Korea
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We evaluated the antibiotic susceptibilities, hemolytic activities, and technological properties of 36 Staphylococcus xylosus strains and 49 S. pseudoxylosus strains predominantly isolated from fermented soybean foods from Korea. Most of the strains were sensitive to chloramphenicol, erythromycin, gentamycin, kanamycin, lincomycin, oxacillin, tetracycline, and trimethoprim. However, 23 strains exhibited potential phenotypic acquired resistance to erythromycin, lincomycin, and tetracycline. Based on breakpoint values for staphylococci from the Clinical and Laboratory Standards Institute, >30% of the isolates were resistant to ampicillin and penicillin G, but the population distributions in minimum inhibitory concentration tests were clearly different from those expected for acquired resistance. None of the strains exhibited clear α- or β-hemolytic activity. S. xylosus and S. pseudoxylosus exhibited salt tolerance on agar medium containing 20% and 22% (w/v) NaCl, respectively. S. xylosus and S. pseudoxylosus strains possessed protease and lipase activities, which were affected by the NaCl concentration. Protease activity of S. pseudoxylosus was strain-specific, but lipase activity might be a characteristic of both species. This study confirms the potential of both species for use in high-salt soybean fermentation, but the safety and technological properties of strains must be determined to select suitable starter candidates.
Abstract
We evaluated the antibiotic susceptibilities, hemolytic activities, and technological properties of 36 Staphylococcus xylosus strains and 49 S. pseudoxylosus strains predominantly isolated from fermented soybean foods from Korea. Most of the strains were sensitive to chloramphenicol, erythromycin, gentamycin, kanamycin, lincomycin, oxacillin, tetracycline, and trimethoprim. However, 23 strains exhibited potential phenotypic acquired resistance to erythromycin, lincomycin, and tetracycline. Based on breakpoint values for staphylococci from the Clinical and Laboratory Standards Institute, >30% of the isolates were resistant to ampicillin and penicillin G, but the population distributions in minimum inhibitory concentration tests were clearly different from those expected for acquired resistance. None of the strains exhibited clear α- or β-hemolytic activity. S. xylosus and S. pseudoxylosus exhibited salt tolerance on agar medium containing 20% and 22% (w/v) NaCl, respectively. S. xylosus and S. pseudoxylosus strains possessed protease and lipase activities, which were affected by the NaCl concentration. Protease activity of S. pseudoxylosus was strain-specific, but lipase activity might be a characteristic of both species. This study confirms the potential of both species for use in high-salt soybean fermentation, but the safety and technological properties of strains must be determined to select suitable starter candidates.
참고문헌 (46)
- Coton E, "Biodiversity of coagulase-negative staphylococci in French cheeses, dry fermented sausages, processing environments and clinical samples", Int. J. Food Microbiol, 137, pp.221~229 (2010)
- Irlinger F, "Safety assessment of dairy microorganisms : coagulase-negative staphylococci", Int. J. Food Microbiol, 126, pp.302~310 (2008)
- Leroy S, "Encyclopedia of Food and Health", pp.140~145 (2016), Academic Press
- Nobuko Hongu, "Korean kimchi: promoting healthy meals through cultural tradition", Journal of Ethnic Foods, 4(3), pp.172~180 (2017), 한국식품연구원
- Donghwa Shin, "Korean traditional fermented soybean products: Jang", Journal of Ethnic Foods, 2(1), pp.2~7 (2015), 한국식품연구원
- 최경근, "메주발효에 관여하는 우량균주의 분리, 동정 및 생육특성", 한국식품영양과학회지, 32(6), pp.818~824 (2003), 한국식품영양과학회
- Kang MJ, "Isolation and identification of microorganisms producing the soy proteinhydrolyzing enzyme from traditional mejus", J. Korean Soc. Agric. Chem. Biotechnol, 43, pp.86~94 (2000)
- Kwon OJ, "The characteristics of bacteria isolated from ordinary Korean soy sauce and soybean paste", Appl. Biol. Chem, 29, pp.422~428 (1986)
- Yoo SK, "Isolation and identification of microorganisms in Korean traditional soybean paste and soybean sauce", Korean J. Appl. Microbiol. Biotechnol, 27, pp.113~117 (1999)
- Jung JY, "Microbial community dynamics during fermentation of doenjang-meju, traditional Korean fermented soybean", Int. J. Food Microbiol, 185, pp.112~120 (2014)
- Jung WY, "Functional characterization of bacterial communities responsible for fermentation of doenjang : a traditional Korean fermented soybean paste", Front. Microbiol, 7, pp.827~ (2016)
- Yi-Seul Kim, "Analyses of Bacterial Communities in Meju, a Korean Traditional Fermented Soybean Bricks, by Cultivation-Based and Pyrosequencing Methods", The Journal of Microbiology, 49(3), pp.340~348 (2011), 한국미생물학회
- Su Yun Lee, "Mass Spectrometry-Based Metabolite Profiling and Bacterial Diversity Characterization of Korean Traditional Meju During Fermentation", Journal of Microbiology and Biotechnology, 22(11), pp.1523~1531 (2012), 한국미생물·생명공학회[DOI]
- Nam YD, "Microbial community analysis of Korean soybean pastes by next-generation sequencing", Int. J. Food Microbiol, 155, pp.36~42 (2012)
- Jeong DW, "Effects of Enterococcus faecium and Staphylococcus succinus starters on the production of volatile compounds during doenjang fermentation", LWT-Food Sci. Technol, 122, pp.108996~ (2020)
- 정도원, "Bacterial Community Migration in the Ripening of Doenjang, a Traditional Korean Fermented Soybean Food", Journal of Microbiology and Biotechnology, 24(5), pp.648~660 (2014), 한국미생물·생명공학회[DOI]
- 장미현, "메주 발효 관련 Bacillus, Enterococcus, Staphylococcus 속 우점종 확인", 한국미생물·생명공학회지, 47(3), pp.359~363 (2019), 한국미생물·생명공학회[DOI]
- MacFadyen AC, "Staphylococcus pseudoxylosus sp. nov., isolated from bovine mastitis", Int. J. Syst. Evol. Microbiol, 69, pp.2208~2213 (2019)
- 홍지수, "Complete genome sequence of biofilm-producing strain Staphylococcus xylosus S170", 미생물학회지, 54(2), pp.167~168 (2018), 한국미생물학회[DOI]
- Simon J. Labrie, "First Complete Genome Sequence of Staphylococcus xylosus, a Meat Starter Culture and a Host to Propagate Staphylococcus aureus Phages", Genome Announcements, 2(4) (2014), American Society for Microbiology[DOI]
- Clinical and Laboratory Standards Institute, "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard—ninth edition M07-A9" (2012), CLSI
- Jeong DW, "Safety and technological characterization of Staphylococcus equorum isolates from jeotgal, a Korean high-salt-fermented seafood, for starter development", Int. J. Food Microbiol, 188, pp.108~115 (2014)
- Jeong DW, "Safety and technological characterization of coagulase-negative staphylococci isolates from traditional Korean fermented soybean foods for starter development", Int. J. Food Microbiol, 236, pp.9~16 (2016)
- 정도원, "젓갈 유래 Coagulase-Negative Staphylococci의 안전성 평가", 한국미생물·생명공학회지, 43(1), pp.84~90 (2015), 한국미생물·생명공학회[DOI]
- Wang YT, "Distribution of antibiotic resistance genes among Staphylococcus species isolated from ready-to-eat foods", J. Food Drug Anal, 27, pp.841~848 (2019)
- "Performance standards for antimicrobial susceptibility testing, 31st edition" (2021)[URL]
- "Breakpoint tables for interpretation of MICs and zone diameter" (2021)[URL]
- "Breakpoint tables for interpretation of MICs and zone diameters, Version 7.0" (2021)[URL]
- "Recommendations 2021" (2021)[URL]
- Clinical and Laboratory Standards Institute, "Performance standards for antimicrobial susceptibility testing; twentieth informational supplement" (2010), CLSI
- Clinical and Laboratory Standards Institute., "Performance standards for antimicrobial susceptibility testing, 27th edition" (2017), CLSI
- Herbert S, "Repair of global regulators in Staphylococcus aureus 8325 and comparative analysis with other clinical isolates", Infect. Immun, 78, pp.2877~2889 (2010)
- Wiegand I, "Agar and broth dilution methods to determine the minimal inhibitory concentration(MIC)of antimicrobial substances", Nat. Protoc, 3, pp.163~175 (2008)
- Yue X, "Microbial diversity and function of soybean paste in East Asia : what we know and what we don’t", Curr. Opin. Food Sci, 37, pp.145~152 (2021)
- Blaiotta G, "Diversity and dynamics of communities of coagulase-negative staphylococci in traditional fermented sausages", J. Appl. Microbiol, 97, pp.271~284 (2004)
- Corbiere Morot-Bizot S, "Monitoring of staphylococcal starters in two French processing plants manufacturing dry fermented sausages", J. Appl. Microbiol, 102, pp.238~244 (2007)
- Leroy S, "Biodiversity of indigenous staphylococci of naturally fermented dry sausages and manufacturing environments of small-scale processing units", Food Microbiol, 27, pp.294~301 (2010)
- Rantsiou K, "Ecology and characterization by molecular methods of Staphylococcus species isolated from fresh sausages", Int. J. Food Microbiol, 97, pp.277~284 (2005)
- Ghebremedhin B, "Genetic classification and distinguishing of Staphylococcus species based on different partial gap, 16S rRNA, hsp60, rpoB, sodA, and tuf gene sequences", J. Clin. Microbiol, 46, pp.1019~1025 (2008)
- Blaiotta G, "Design and evaluation of specific PCR primers for rapid and reliable identification of Staphylococcus xylosus strains isolated from dry fermented sausages", System. Appl. Microbiol, 26, pp.601~610 (2003)
- Poyart C, "Rapid and accurate species-level identification of coagulase-negative staphylococci by using the sodA gene as a target", J. Clin. Microbiol, 39, pp.4296~4301 (2001)
- Marty E, "Prevalence of antibiotic resistance in coagulase-negative staphylococci from spontaneously fermented meat products and safety assessment for new starters", Int. J. Food Microbiol, 159, pp.74~83 (2012)
- 공하람, "Complete genome sequence of Staphylococcus pseudoxylosus 14AME19 exhibiting a weak hemolytic activity", 미생물학회지, 57(2), pp.109~111 (2021), 한국미생물학회[DOI]
- Jeong DW, "Genomic insights into the virulence and salt tolerance of Staphylococcus equorum", Sci. Rep, 7, pp.5383~ (2017)
- Leroy F, "Functional meat starter cultures for improved sausage fermentation", Int. J. Food Microbiol, 106, pp.270~285 (2006)
- EFSA Panel on Biological Hazards, "Update of the list of QPS-recommended biological agents intentionally added to food or feed as notified to EFSA 13 : suitability of taxonomic units notified to EFSA until September 2020", EFSA J, 19, pp.e06377~ (2021)
