Edwardsiella tarda extracellular products (ECPs)의 인위 투여에 따른 넙치, Paralichthys olivaceus의 생체 반응
Effect of extracellular products (ECPs) of Edwardsiella tarda on olive flounder, Paralichthys olivaceus
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본 연구는 강독성 균주인 E. tarda PoKF-000623가 생산하는 세포외생성물 (ECPs)을 넙치에 투여되었을 때 나타나는 생리학적 및 면역학적 영향을 조사하고자 하였다. E. tarda PoKF-000623 균주가 생성하는 ECPs의 넙치 (평균체중, 5.6g)에 대한 96hr-LD50 은 약 0.714㎍ g fish-1 이었으며, ECPs의 주사에 의한 폐사는 고농도 (40㎍ fish-1) 뿐만 아니라 저농도 (4㎍ fish-1)에서도 지속적으로 발생하였다. 평균 체중 59.1 g 크기의 넙치에 각각 0, 4 and 40㎍ ECPs fish-1 의 농도로 근육 주사한 후, 혈청 중 glucose 농도와 total protein 농도 및 신장 식세포의 활성을 조사하였다. 그 결과 고농도의 ECPs 주사 시험구의 넙치에서 혈중 glucose 농도와 total protein 농도가 대조구에 비하여 유의적으로 높게 나타났다. 그러나 세포성 면역 기구인 신장 식세포의 CL 반응과 세균 살해능은 대조구에 비하여 유의적으로 저하되었다. 따라서 넙치에 고농도의 E. tarda ECPs를 투여하면 생체 지표의 일부에 영향을 미치는 것으로 확인되었고 면역 활성, 특히 신장 식세포 활성의 억제가 edwardsielosis의 발병에 영향을 미치는 것으로 사료된다.
Abstract
The effect of extracellular products (ECPs) prepared from highly virulent Edwardsiella tarda PoKF-000623 on the physiological and the immunological function in the olive flounder, Paralichthys olivaceus was evaluated. The virulence of ECPs on the fish, 5.6g of average body weight was detected 0.714 ㎍ g fish-1 as 96h-LD50 by intraperitonial injection. After intramuscularly injected with 0, 4 and 40 ㎍ ECPs fish-1to fish average sized 59.1 g of body weight, the fish were measured the glucose and total protein concentration in sera, and immune activity of kidney macrophage. The fish injected with 40 ㎍ protein fish-1 showed a significant increase in the glucose and total protein concentration in sera. But, the fish were changed in cellular immune response as lowed chemiluminescence response and bactericidal activity of head kidney macrophage. These results were suggested that ECPs of E. tarda could be effect on the physiological and the immunological factors, especially in the function of kidney macrophage concerning to edwardsiellosis infection.
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