다양한 농도의 에드워드 백신액에 대한 넙치, Paralichthys olivaceus의 침지 투여 효과
Effects of immersion vaccination in different concentration of edwardsiellosis vaccine on olive flounder, Paralichthys olivaceus
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참고문헌
초록
본 연구는 양식 넙치, Paralichthys olivaceus에서 희석 백신의 장시간 처리 효과를 조사하였다. 넙치에 상업용 에드워드 백신 (5㎎/ℓ)을 100, 10-1, 10-2 and 10-3로 희석하여 2분 (short immersion, SI)과 24시간 (prolonged immersion, PI) 침지한 다음 시험어의 비특이적 면역계 (혈청 라이소자임, 살균능)와 특이적 면역계 (응집항체가)의 활성과 Edwardsiella tarda 공격에 대한 방어력의 변화를 조사하였다. 그 결과 에드와드 백신을 10-1과 10-2로 희석하여 장시간 침지 투여 시험구가 고농도 백신의 단시간 침지 시험구에 비하여 혈청 라이소자임 활성과 보체의 살균능이 높았으며 에드워드균 감염에 대한 방어력도 높았다. 따라서 희석 백신을 장시간 침지 처리하는 방법은 백신 효과를 높일 수 있을 것으로 기대된다.
Abstract
This study was performed to investigate the effects of prolonged immersion vaccination in a dilute vaccine on the farmed olive flounder, Pararlichthys olivaceus. Juvenile olive flounders were immersed in the several diluted (100, 10-1, 10-2 and 10-3) commercial edwardsiellosis bacterins (5㎎/ℓ) for 2min (short immersion, SI) and 24 h (prolonged immersion, PI). After immunization, fish were analyzed to investigate on non-specific (serum lysozyme and bactericidal activity), specific immune responses (antibody agglutinin titer) and resistances against Edwardsiella tarda infection. The PI of the commercial vaccine diluted with 10-1 and 10-2 showed better results over the untreated control in terms of serum lysozyme, bactericidal activities and resistance against E. tarda infection. These results suggest that the prolonged treatments of the diluted vaccine might be a new approach improving the vaccination potential.
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