멍게의 물렁증 발생에 미치는 용존산소의 영향
Effect of Dissolved Oxygen on Occurrence of Tunic Softness Syndrome in Sea Squirt Halocynthia roretzi, Tongyeong, South Coast of Korea
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초록
This study analyzed the occurrence of tunic softness, survival rate, metabolic rate and histopathologic changes arising from the effect of hypoxic environment in order to find the causes of occurrences of tunic softness, which manifests as the key phenomenon of mass mortality of Halocynthia roretzi. Regarding the survival of H. roretzi with reduction in dissolved oxygen, all the entities died on the 4th day of exposure to the dissolved oxygen concentration of 2 mg L‑¹ while 50% mortality was observed on the 5th day of exposure to the dissolved oxygen concentration of 3mg L‑¹. Therefore the 5 days-LC50 was found to be 3.55 mg L‑¹ (1.86~4.96 mg L‑¹). However,occurrence of tunic softness was not observed during the period of exposure to low oxygen concentration. The oxygen consumption rate significantly decreases at the dissolved oxygen concentration of less than 5 mg L‑¹ in comparison to the control group. Therefore, it is presumed that H. roretzi controls the respiration rate for prescribed period of time when exposed to hypoxic environment. Regarding the histopathologic changes in the gill, digestive gland and cyst of H. roretzi due to hypoxic environment, necrosis of epithelial layer, in filtration of blood cells, and condensation of nucleus that compose each of the organs were observed. Regarding morphological changes, the decrease in volume with shrinking of the tunic, discoloration of the internal organs and necrosis of gill and hepatopancreas were observed.
Abstract
This study analyzed the occurrence of tunic softness, survival rate, metabolic rate and histopathologic changes arising from the effect of hypoxic environment in order to find the causes of occurrences of tunic softness, which manifests as the key phenomenon of mass mortality of Halocynthia roretzi. Regarding the survival of H. roretzi with reduction in dissolved oxygen, all the entities died on the 4th day of exposure to the dissolved oxygen concentration of 2 mg L‑¹ while 50% mortality was observed on the 5th day of exposure to the dissolved oxygen concentration of 3mg L‑¹. Therefore the 5 days-LC50 was found to be 3.55 mg L‑¹ (1.86~4.96 mg L‑¹). However,occurrence of tunic softness was not observed during the period of exposure to low oxygen concentration. The oxygen consumption rate significantly decreases at the dissolved oxygen concentration of less than 5 mg L‑¹ in comparison to the control group. Therefore, it is presumed that H. roretzi controls the respiration rate for prescribed period of time when exposed to hypoxic environment. Regarding the histopathologic changes in the gill, digestive gland and cyst of H. roretzi due to hypoxic environment, necrosis of epithelial layer, in filtration of blood cells, and condensation of nucleus that compose each of the organs were observed. Regarding morphological changes, the decrease in volume with shrinking of the tunic, discoloration of the internal organs and necrosis of gill and hepatopancreas were observed.
참고문헌 (34)
- Aquacop EB, "Effects of dissolved oxygen concentration on survival and growth of Penaeus vannamei and Penaeus stylirostris", J. World Aquacult. Soc, 19, pp.12A~ (1988)
- Armsworthy SL, "Feeding activity, absorption efficiency and suspension feeding processes in the ascidian, Halocynthia pyriformis (Stolodobranchia: Ascidiacea): responses to variations in die quantity and qualitity", J. Exp. Mar. Biol. Ecol, 260, pp.41~69 (2001)
- Bayne BL., "The responses of three species of bivalve mollusc to declining oxygen tension at reduced salinity", Comp. Biochem. Physiol, 45A, pp.793~806 (1973)
- Bayne BL, "Physiological energetics of marine molluscs. In the mollusca, Vol. 4", pp.407~515 (1983), Academic Press
- Bohle B., "Effects of adaptation to reduced salinity on filtration activity and growth of mussels (Mytilus edulis)", J. Exp. Mar. Biol. Ecol, 10, pp.41~49 (1972)
- Choi DL, "First report on histology and ultrastructure of an intrahemocytic paramyxean parasite (IPP) from tunicate Halocynthia roretzi in Korea", Dis. Aquat. Organ, 72, pp.65~69 (2006)
- deFur PL, "Respiratory responses if the blue crab Callinects sapidus in long term hypoxia", Biol. Bull, 178, pp.46~54 (1990)
- DeZwaan A, "Anaerobic metabolism in bivalvia (Mollusca). Characteristics of anaerobic metabolism", Comp. Biochem. Physiol, 54B, pp.313~317 (1976)
- Finney DJ., "Probit analysis", pp.333~ (1971), Cambridge University Press
- Herreid CF., "Hypoxia in invertebrates", Comp. Biochem. Physiol, 67A, pp.311~320 (1980)
- Hirai E., "An outline of the development of Halocynthia roretzi Drasche", Sci. Rep. Tohoku Imp. Univ. Biol, 16, pp.217~232 (1941)
- Hirai E., "On the changes of the adhesive papillae of the larvae of an ascidian Halocynthia roretzi", Bull. Mar. Biol. St. Asamushi, 12, pp.9~11 (1965)
- Jang YJ, "Studies on the early growth of the sea squirt, Halocynthia roretzi (Drasche)", Bull. Fish. Res. Dev. Agency, 21, pp.69~76 (1979)
- Jiang AL, "Physiological energetics of the ascidian Styela clava in relation to body size and temperature", Comp. Biochem. Physiol. part A, 149, pp.129~136 (2008)
- Kim BS, "Gametogenesis, Gonadal Development and Maturation of the Sea Squirt, Halocynthia roretzi", Dev. Reprod, 5, pp.137~144 (2001)
- Kim SH, "Hasllibacter halocynthiae gen.nov., sp. nov., a nutriacholic acid-producing bacterium isolated from the marine ascidian Halocynthia roretzi", Internal J. System. Evo. Micro, 62, pp.624~631 (2012)
- Kumagai A, "Soft tunic syndrome in the edible ascidian Halocynthia roretzi is caused by a kinetoplastid protist", Dis. Aquat. Org, 95, pp.153~161 (2010)
- Kumagai, A, "Mass mortality of cultured ascidians Halocynthia roretzi associated with softening of the tunic and flagellate-like cells", Dis. Aquat. Org, 90, pp.223~234 (2010)
- Maruo NA, "The effect of hypoxia on blood pH and lactate levels in Macrobrachiium rosenbergii (de Man)", Comp. Biochem. Physiol, 77A, pp.627~630 (1984)
- McMahon BR, "Acid-base changes during recovery from disturbance and during long term hypoxic exposure in the lobster, Homarus vulgaris", J. Exp. Zool, 205, pp.361~370 (1978)
- Morris S, "Hemolymph respiratory gas, acid and ion status of the amphibious purple shore crab Leptograpsus variegates (Fabricus) during immersion and environmental hypoxia", J. Crust. Biol, 16, pp.253~266 (1996)
- Pamatmat MM, "Faculaive anaerobiosis of benthos. In, marine benthic dymanics", pp.69~90 (1980), University of south Carolina
- Ryland JS., "A circadian rhythm in the tropical ascidian Diplosoma virens (Ascidiacea : Didemnidae)", J. Exp. Mar. Biol. Ecol, 138, pp.217~225 (1990)
- 신윤경, "Physiological Responses of the Ark Shell Scapharca broughtonii (Bivalvia: Arcidae) to Decreases in Salinity", Fisheries and Aquatic Sciences, 9(4), pp.153~159 (2006), 한국수산과학회
- 신윤경, "통영산 물렁증 멍게 피막의 편모충 감염", 한국어병학회지, 24(3), pp.197~204 (2011), 한국어병학회
- 신윤경, "멍게(Halocynthia roretzi)의 계절별 생리적 변화 및 에너지 수지", 한국수산과학회지, 44(4), pp.366~371 (2011), 한국수산과학회
- 신윤경, "꼬막, Tegillarca granosa (Linnaeus)의 용존산소 변화에 따른 생리적 반응", 한국수산과학회지, 35(5), pp.485~489 (2002), 한국수산과학회
- Shin YK, "Effects of the dissolved oxygen concentration on the physiology of the manila clam, Ruditapes philippinarum", J. Korean Fish. Soc, 34, pp.190~193 (2001)
- Solorzano L., "Determination of ammonia in natural waters by the phenolhypochlorite method", Limnol. Oceanogr, 14, pp.799~801 (1969)
- Truchot JP., ". Changements de l’état acide-base du sang en fonction de l’oxygénation de l’eau chez le crabe Carcinus maenas (L.)", J. Physiol, 70, pp.583~592 (1975)
- Yoo SK, "On the growth of the sea squirt (Halocynthia roretzi) from artificial seed", Korean J. Aquacult, 1, pp.75~84 (1988)
- Yoo SK, "Influence of water temperature on spawning induction, egg development and sead collection of sea squirt, Halocynthia roretzi", Korean J. Aquacult, 3, pp.79~88 (1990)
- Wilbur KM, "Physiology of mollusca", pp.201~203 (1966), Academic press
- Winddows J, "Physiological procedures. In: The Effects of Stress and Pollution on Marine Animals", pp.161~178 (1985), Academic Press