Comparison of lymphocystis disease virus proteins between marine and freshwater fish
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.
Abstract
Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.
참고문헌 (18)
- Anderson, I.G., "Iridovirus-like virions in imported dwarf gourami (Colisa lalia) with systemic amoebiasis", Aust. Vet. J., 70, pp.66~67 (1993)
- Cano, I., "Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head sea bream (Sparus aurata, L.) using an immunoblot technique", Vet. Microbiol., 113, pp.137~141 (2006)
- Darai, G., "Analysis of the genome of fish lymphocystis disease virus isolated directly from epidermal tumours of pleuronectes", Virology, 126, pp.466~479 (1983)
- Flugel, R.M., "Viral proteins and adenosine triphosphate phosphohydrolase activity of fish lymphocystis disease virus", Virology, 122, pp.48~55 (1982)
- Garcia-Rosado, E., "Protein and glycoprotein content of lymphocystis disease virus (LCDV)", Int. Microb., 7, pp.121~126 (2004)
- Iwamoto, R., "Isolation and characterization of the Japanese flounder (Paralichthys olivaceus) lymphocystis disease virus", J. Aquat. Animal Health, 14, pp.114~123 (2002)
- Kitamura, S.I., "A new genotype of lymphocystivirus, LCDV-RK, from lymphocystis diseased rockfish", Arch. Virol., 151, pp.607~615 (2006)
- Shin-Ichi Kitamura, "Differentiation of Lymphocystis Disease Virus Genotype by Multiplex PCR", The Journal of Microbiology, 44(2), pp.248~253 (2006), 한국미생물학회
- Laemmli, U.K., "Cleavage of structural proteins during the assembly of the head of bacteriophage T4", Nature, 227, pp.680~685 (1970)
- Samalecos, C.P., "Analysis of the structure of fish lymphocystis disease virions from skin tumors of Pleuronectes", Arch. Virol., 91, pp.1~10 (1986)
- Schnitzler, P., "Identification of the gene encoding the major capsid protein from fish lymphocystis disease virus", J. Gen. Virol., 74, pp.2143~2150 (1993)
- Tidona, C.A., "The complete DNA sequence of lymphocystis disease virus", Virology, 230, pp.353~362 (1997)
- Tidona, C.A., "Is the major capsid protein of iridoviruses a suitable target for the study of viral evolution?", Virus Genes, 16, pp.59~66 (1998)
- Walker, R., "Fine structure of lymphocystis disease virus of fish", Virology, 18, pp.503~505 (1962)
- Webby, R., "Sequence comparison of the major capsid protein gene from 18 diverse iridoviruses", Arch. Virol., 143, pp.1949~1966 (1998)
- Wolf, K., "Lymphocystis disease. In: Fish viruses and fish viral diseases", pp.268~291 (1988), Cornell University Press
- Zhang, Q.Y., "Complete genome sequence of lymphocystis disease virus isolated from China", J. Virol., 78, pp.6982~6994 (2004)
- Zwillenberg, L.D., "Ultrastructure of lymphocystis virus", J. Virol., 2, pp.393~399 (1968)