Phytochemicals and antioxidant capacity of some tropical edible plants
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Objective: To find biological functions such as antibacterial and antioxidant activities in several tropical plants and to investigate the possibility of antibiotic substitute agents to prevent and treat diseases caused by pathogenic bacteria. Methods: Plants such as Poncirus trifoliata fruit (Makrut), Zingiber officinale Rosc (Khing), Areca catechu L. (Mak), Solanum melongena L. I (Makkhuayao), and Solanum melongena L. II (Makhurapro) were extracted by methanol, n-hexane, chloroform, ethyl acetate, butanol and water. The free radical scavenging activities were measured using 2-diphenyl-2-picryl hydrazyl photometric assay. Antibacterial activities with a minimum inhibitory concentration (MIC) were observed by agar diffusion assay against pathogenic strains of Escherichia coli, Burkholderia sp., Haemopilus somnus, Haemopilus parasuis, Clostridium perfringens, and Pantoea agglomerans. Results: Poncirus trifoliata fruit methanol extract showed antibacterial activities against gram-negative and gram-positive pathogens. Additionally, this showed the strongest antibacterial activity against Burkholderia sp. and Haemopilus somnus with MIC 131 μg/mL, respectively. Areca catechu L. water extract showed antibacterial activities against Burkholderia sp., Haemopilus somnus, and Haemopilus parasuis. The MIC value for Haemopilus parasuis was 105 μg/mL in this. Antioxidant activity of Zingiber officinale Rosc n-hexane extract showed 2.23 mg/mL effective concentration 50% (EC50) value was the highest activity among tropical plants extracts. Total polyphenol content in Zingiber officinale Rosc methanol extract was 48.4 μg/mL and flavonoid content was 22.1 μg/mL showed the highest values among tested plants extracts. Conclusion: Taken together, these results suggest that tropical plants used in this study may have a potential benefit as an alternative antibiotics agent through their antibacterial and antioxidant activities.
Abstract
Objective: To find biological functions such as antibacterial and antioxidant activities in several tropical plants and to investigate the possibility of antibiotic substitute agents to prevent and treat diseases caused by pathogenic bacteria. Methods: Plants such as Poncirus trifoliata fruit (Makrut), Zingiber officinale Rosc (Khing), Areca catechu L. (Mak), Solanum melongena L. I (Makkhuayao), and Solanum melongena L. II (Makhurapro) were extracted by methanol, n-hexane, chloroform, ethyl acetate, butanol and water. The free radical scavenging activities were measured using 2-diphenyl-2-picryl hydrazyl photometric assay. Antibacterial activities with a minimum inhibitory concentration (MIC) were observed by agar diffusion assay against pathogenic strains of Escherichia coli, Burkholderia sp., Haemopilus somnus, Haemopilus parasuis, Clostridium perfringens, and Pantoea agglomerans. Results: Poncirus trifoliata fruit methanol extract showed antibacterial activities against gram-negative and gram-positive pathogens. Additionally, this showed the strongest antibacterial activity against Burkholderia sp. and Haemopilus somnus with MIC 131 μg/mL, respectively. Areca catechu L. water extract showed antibacterial activities against Burkholderia sp., Haemopilus somnus, and Haemopilus parasuis. The MIC value for Haemopilus parasuis was 105 μg/mL in this. Antioxidant activity of Zingiber officinale Rosc n-hexane extract showed 2.23 mg/mL effective concentration 50% (EC50) value was the highest activity among tropical plants extracts. Total polyphenol content in Zingiber officinale Rosc methanol extract was 48.4 μg/mL and flavonoid content was 22.1 μg/mL showed the highest values among tested plants extracts. Conclusion: Taken together, these results suggest that tropical plants used in this study may have a potential benefit as an alternative antibiotics agent through their antibacterial and antioxidant activities.
참고문헌 (41)
- Abimbola KA, "Current status on biotyping antibiogram and plasmid profiles of E. coli isolates", East Afr Med J, 70, pp.207~213 (1993)
- Khulbe K, "Antibacterial activity of Boenninghausenia albiflora Reichb. (Rutaceae)", Afr J Biotechnol, 8, pp.6346~6348 (2009)[DOI]
- Islam K, "Antimicrobial activity of ginger (Zingiber officinale) extracts against food-borne pathogenic bacteria", Int J Sci Environ Technol, 3, pp.867~871 (2014)
- Wegener HC, "Antibiotics in animal feed and their role in resistance development", Curr Opin Microbiol, 6, pp.439~445 (2003)[DOI]
- Cowan MM, "Plant products as antimicrobial agents", Clin Microbiol Rev, 12, pp.564~582 (1999)
- Gautam R, "Indian medicinal plants as a source of antimycobacterial agents", J Ethnopharmacol, 110, pp.200~234 (2007)[DOI]
- Prasad SK, "Antidiarrhoeal evaluation of root extract, its bioactive fraction, and lupinifolin isolated from Eriosema chinense", Planta Med, 79, pp.1620~1627 (2013)[DOI]
- Esquenazi D, "Antimicrobial and antiviral activities of polyphenolics from Cocos nucifera Linn. (Palmae) husk fiber extract", Res Microbiol, 153, pp.647~652 (2002)[DOI]
- Wannissorn B, "Antibacterial properties of essential oils from Thai medicinal plants", Fitoterapia, 76, pp.233~236 (2005)[DOI]
- J.H. Lee, "Investigation on Antibacterial and Antioxidant Activities, Phenolic and Flavonoid Contents of Some Thai Edible Plants as an Alternative for Antibiotics", Animal Bioscience, 27(10), pp.1461~1468 (2014), 아세아·태평양축산학회[DOI]
- Yi JM, "Poncirus trifoliata fruit induces apoptosis in human promyelocytic leukemia cells", Clin Chim Acta, 340, pp.179~185 (2004)[DOI]
- Chen IN, "Antioxidant and antimicrobial activity of Zingiberaceous plants in Taiwan", Plants Foods Hum Nutr, 63, pp.15~20 (2008)[DOI]
- 이강파, "The anti-inflammatory effect of Indonesian Areca catechu leaf extract in vitro and in vivo", Nutrition Research and Practice, 8(3), pp.267~271 (2014), 한국영양학회
- Bernardo JS, "Eggplant (Solanum melongena L.) peel as a potential functional ingredient in pan de sal", J Nutr Food Sci, 6(6(Suppl)) (2016)
- 홍희옥, "Characteristics of Chinese chives (Allium tuberosum) fermented by Leuconostoc mesenteroides", Applied Biological Chemistry, 59(3), pp.349~357 (2016), 한국응용생명화학회
- Wiegand I, "Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances", Nat Protoc, 3, pp.163~175 (2008)[DOI]
- Alviano WS, "In vitro antioxidant potential of medicinal plant extracts and their activities against oral bacteria based on Brazilian folk medicine", Arch Oral Biol, 53, pp.545~552 (2008)[DOI]
- Tadic VM, "Anti-inflammatory, gastroprotective, free-radical-scavenging, and antimicrobial activities of hawthorn berries ethanol extract", J Agric Food Chem, 56, pp.7700~7709 (2008)[DOI]
- Wolfe K, "Antioxidant activity of apple peels", J Agric Food Chem, 51, pp.609~614 (2003)[DOI]
- Kiselova Y, "Correlation between the in vitro antioxidant activity and polyphenol content of aqueous extracts from Bulgarian herbs", Phytother Res, 20, pp.961~965 (2006)[DOI]
- Ordon AAL, "Antioxidant activities of Sechium edule (Jacq.) Swart extracts", Food Chem, 97, pp.452~458 (2006)[DOI]
- Harris FW, "The Haemophilus somnus disease complex (Hemophilosis): a review", Can Vet J, 30, pp.816~822 (1989)
- Sandal I, "A genomic window into the virulence of Histophilus somni", Trends Microbiol, 18, pp.90~99 (2010)[DOI]
- Niilo L, "Clostridium perfringens in animal disease: a review of current knowledge", Can Vet J, 21, pp.141~148 (1980)
- Cruz AT, "Pantoea agglomerans, a plant pathogen causing human disease", J Clin Microbiol, 45, pp.1989~1992 (2007)[DOI]
- Al-Bayati NJM, "In-vitro antibacterial and antifungal effect of areca nut extract", Res J Pharm Biol Chem Sci, 7, pp.282~286 (2016)
- Li R, "Genotypic and phenotypic characterization of antimicrobial-resistant Escherichia coli from farmraised diarrheic sika deer in Northeastern China", Plos One, 8, pp.e73342~ (2013)[DOI]
- Rahman A, "In vitro control of plant pathogenic Xanthomonas spp. Using Poncirus trifoliata rafin", EXCLI J, 13, pp.1104~1110 (2014)
- Danciu C, "Evaluation of phenolic profile, antioxidant and anticancer potential of two main representants of Zingiberaceae family against B164A5 murine melanoma cells", Biol Res, 48, pp.1~ (2015)[DOI]
- Ghasemzadeh A, "Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe)", Molecules, 15, pp.4324~4333 (2010)[DOI]
- Sasidharan S, "Extraction, isolation and characterization of bioactive compounds from plants' extracts", Afr J Tradit Complement Altern Med, 8, pp.1~10 (2011)
- Kim DH, "Anti-Helicobacter pylori activity of the metabolites of poncirin from Poncirus trifoliata by human intestinal bacteria", Biol Pharm Bull, 22, pp.422~424 (1999)[DOI]
- Ali BH, "Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research", Food Chem Toxicol, 46, pp.409~420 (2008)[DOI]
- Siddaraju MN, "Inhibition of gastric H+,K+-ATPase and Helicobacter pylori growth by phenolic antioxidants of Zingiber officinale", Mol Nutr Food Res, 51, pp.324~332 (2007)[DOI]
- Tohma H, "Antioxidant activity and phenolic compounds of ginger (Zingiber officinale Rosc.) determined by HPLC-MS", MS, 2017, pp.11, 556~566
- Rackova L, "Redox properties of ginger extracts: Perspectives of use of Zingiber officinale Rosc. as antidiabetic agent", Interdiscip Toxicol, 6, pp.26~33 (2013)[DOI]
- 유동준, "The relaxing effect of Poncirus fructus and its flavonoid content on porcine coronary artery", Laboratory Animal Research, 31(1), pp.33~39 (2015), 한국실험동물학회
- Hamsar MN, "Antioxidant activity and the effect of different parts of areca catechu extracts on Glutathione-S-Transferase activity in vitro", Free Radic Antioxid, 1, pp.28~33 (2011)[DOI]
- Chavan YV, "Separation of polyphenols and arecoline from areca nut (Areca catechu L.) by solvent extraction, its antioxidant activity, and identification of polyphenols", J Sci Food Agric, 93, pp.2580~2589 (2013)[DOI]
- Akanitapicha P, "Antioxidant and hepatoprotective activity of five eggplant varieties", Food Chem Toxicol, 48, pp.3017~3021 (2010)[DOI]
- Azuma K, "Structures and antioxidant activity of anthocyanins in many accessions of eggplant and its related species", J Agric Food Chem, 56, pp.10154~10159 (2008)[DOI]