Ampicillin의 경구, 주사 및 약욕 투약에 따른 넙치 혈액에서의 잔류량 변화

Abstract

The residue levels of ampicillin (AM) were studied after oral administration, intramuscular injection and dipping to cultured olive flounder, Paralichthys olivaceus (average 300 g) at 20±1.0℃. The concentrations of AM in the plasma were determined by HPLC-UV detector. The average recoveries of AM in spiked samples between 0.01∼10 ppm were ranging from 84.45% to 91.26% for plasma. The Limit of detection for AM was 0.05 ppm by using this method. Plasma concentrations of AM were determined after oral dosage (10, 20 and 40 ㎎/㎏ body weight), intramuscular injection (5, 10 and 20 ㎎/㎏ body weight) and dipping (10, 20 and 40 ppm; 1 h). Samples were taken at 1, 3, 5, 10, 15, 24, 30, 48, 96, 144, 216, 264 and 360 h post-dose. In oral dosage of 10, 20 and 40 ㎎/㎏, it's peak plasma concentrations, which attained at 10 h post-dose in all doses, was 3.62±0.97, 5.20±0.70 and 11.18±0.87 ㎍/㎖, respectively. Those of AM were not measurable at 144, 360 and 360 h post-dose, respectively. In intramuscular injection of 5, 10 and 20 ㎎/㎏, it's peak plasma concentrations, which attained at 5 h post-dose in all doses, was 6.92±1.29 ㎍/㎖, 9.89±2.22 ㎍/㎖ and 19.85±2.97 ㎍/㎖, respectively. Those of AM were not measurable at 216, 264 and 264 h post-dose, respectively. In dipping of 10, 20 and 40 ppm, it's peak plasma concentrations, which attained at 3 h post-dose in all doses, were 4.39±1.10, 9.57±1.51 and 11.61±1.92 ㎍/㎖, respectively. Those of AM were not measurable at 264, 264 and 360 h post-dose, respectively. Therefore, the plasma distribution and elimination levels of AM in olive flounder were dosage-dependant manner in all administration routes.