볼락(Sebastes inermis)에서 분리된 비브리오속 세균의 동정
Identification of Vibrio species isolated from dark-banded rockfish (Sebastes inermis)
1
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.
Abstract
We experienced mortality of dark-banded rockfish (Sebastes inermis) maintained in the rearing facility located in Gangneung, after transportation. Moribund fish showed various symptoms such as exophthalmia, skin ulcers, tail rots, gill rots, discoloration of liver with petechiae, yellowish fluid in intestines and ascites. Two different colonies were dominantly appeared after spreading the lesions on the agar plates and incubation. One isolate (SI_1) showed swarming movement on TSA, and formed yellow colonies on TCBS agar. The other (SI_2) showed no swarming motility and green colonies on TCBS agar. Both of them were Gram-negative. All of these results are similar with those of Genus Vibrio. They were identified as V. harveyi and V. gigantis by PCR with subsequent sequencing of 3 different genes (16 rDNA, recA, rpoA). V. harveyi is well-known as a serious pathogen of marine fish and invertebrates, while V. gigantis is known to be often isolated from marine invertebrates, but the pathogenicity is still unknown. We suspect V. harveyi as the cause of the mortality of dark-banded rockfish, but challenge experiments with these 2 Vibrio species are thought to be necessary to make a clear conclusion.
참고문헌 (16)
- 최희정, "양식산 볼락, Sebastes inermis Cuvier의 성장 특성", 한국양식학회지, 18(3), pp.147~153 (2005), 한국양식학회
- 최혜승, "2006~2008년 남해안 통영∙거제해역의 양식 조피볼락, Sebastes schlegeli 병원체 감염 현황", 한국어병학회지, 23(1), pp.27~35 (2010), 한국어병학회
- 곽우석, "볼락, Sebastes inermis 자ㆍ치어의 성장에 따른 소화효소 활성 변화", 한국양식학회지, 19(2), pp.125~132 (2006), 한국양식학회
- 강가현, "2018년 경남 양식어류에서 검출된 병원체 모니터링", 한국수산과학회지, 52(5), pp.539~546 (2019), 한국수산과학회[DOI]
- 김석렬, "자연산 및 양식산 볼락, Sebastes inermis 의 장내세균총 및 장내세균의 약제내성 비교", 한국어병학회지, 20(3), pp.221~227 (2007), 한국어병학회
- Le Roux F., "Vibrio gigantis sp. nov., isolated from haemolymph of cultured oysters (Crassostrea gigas)", Int. J. Syst. Evol. Microbiol., 55, pp.2251~2255 (2005)
- Masuda, H., "The fishes of the Japanese Archipelago, Vol. 1", pp.437~ (1984), Tokai University Press
- Noguerola I., "Identification of Vibrio spp. with a set of dichotomous keys", J. Appl. Microbiol., 105, pp.175~185 (2008)
- 오승용, "대형 수조에서 볼락 종묘 생산에 따른 수질 환경의 변화", 한국양식학회지, 19(1), pp.25~32 (2006), 한국양식학회
- Ortigosa, M., "Numerical taxonomy of Vibrionaceae isolated from oysters and seawater along an annual cycle", System. Appl. Microbiol., 17, pp.216~225 (1994)
- 손한창, "Identification of Vibrio species isolated from cultured olive flounder (Paralichthys olivaceus) in Jeju Island, South Korea", Fisheries and Aquatic Sciences, 22(3), pp.1~8 (2019), 한국수산과학회[DOI]
- Thompson F. L., "Phylogeny and molecular identification of Vibrios on the basis of multilocus sequence analysis", Appl. Environ. Microbiol., 71, pp.5107~5115 (2005)
- Weisburg W. G., "16S ribosomal DNA amplification of phylogenetic study", J. Bacteriol., 173, pp.697~703 (1991)
- 원경미, "Vibrio harveyi의 미생물학적 특성", 한국어병학회지, 20(3), pp.237~247 (2007), 한국어병학회
- 원경미, "Characterization of Vibrio harveyi, the Causal Agent of Vibriosis in Cultured Marine Fishes in Korea", Fisheries and Aquatic Sciences, 9(3), pp.123~128 (2006), 한국수산과학회
- Zhang X. H., "Vibrio harveyi:A serious pathogen of fish and invertebrates in mariculture", Mar. Life Sci. Technol., 2, pp.231~245 (2020)