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In vitro에서 Photobacterium damselae subsp. damselae와 Vibrio 속 세균의 병원성 비교

Comparative study of Photobacterium damselae subsp. damselae and Vibrio on pathogenicity in vitro

김진우|김명석|박수일|권문경|조병열
수산생명의학회 (Aquatic Life Medical Science)|2007.04|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제20권 제1호|pp. 71-81

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초록

넙치, Paralichthys olivaceus 병어에서 분리된 Photobacterium damselae subsp. damselae와 Vibrio spp.(V. anguillarum, V. splendidus, V. harveyi and V. ordalii)의 병원성을 in vitro에서 조사하였다. Extracellular products (ECPs)의 성상은 효소 활성, 양과 넙치 적혈구에 대한 용혈능과 cell-line에 대한 세포 독성을 조사하였다. 그리고 세균의 internalization에서 signal transduction pathways를 signal transduction inhibitor를 이용하여 조사하였다.P. damselae는 phospholipase 활성, 넙치 적혈구에 대한 용혈능, 세포독성이 높게 나타났으며, 분리된 vibrios 균주에 비하여 internalization에서 다양한 경로를 나타내었다. 따라서 이러한 활성은 P. damselae의 병원성과 관련이 있을 것으로 생각된다.

Abstract

Photobacterium damselae subsp. damselae and 4 Vibrio spp.(V. anguillarum, V. splendidus, V. harveyi and V. ordalii) were isolated from the diseased olive flounders, Paralichthys olivaceus. The isolates were tested on the pathogenicity in vitro. The properties of extracellular products(ECPs) were investigated with enzymatic activities, hemolytic activities toward the sheep and olive flounder erythrocytes, and cytotoxicity activities on the cell-line. And potential signal transduction pathways of the bacterial internalization were detected by using signal transduction inhibitors. P. damselae was high in phospholipase activity, hemolytic activity to olive flounder erythrocytes and cytotoxicity activity. And P. damselae had diversified internalizing pathways as compared to isolated vibrios. Therefore, these activities may be related with pathogenicity of P. damselae.

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관련 논문AI추천

In vivo에서 Photobacterium damselae subsp. damselae와 Vibrio 속 세균의 병원성 비교권문경, 박수일, 조병열수산생명의학회 · 한국어병학회지2009 · 제22권 제2호넙치, Paralichthys olivaceus에서 병원성 Photobacterium damselae subsp. damselae의 분리권문경, 박상언, 박수일, 방종득수산생명의학회 · 한국어병학회지2005 · 제18권 제3호Vibrio harveyi에서 분리한 extracellular products (ECPs)의 특성과 독성김이청, 박수일, 변순규, 원경미, 이배익, 이종하, 최정현, 허민도수산생명의학회 · 한국어병학회지2008 · 제21권 제2호배양 조건이 Photobacterium damselae subsp. damselae의 미생물학적 성상 및 병원성에 미치는 영향권문경, 박명애, 조병열수산생명의학회 · 한국어병학회지2009 · 제22권 제3호우리나라 양식 강도다리, Platichthys stellatus에서 분리된 Photobacterium damselae subsp. piscicida의 특성김진우, 문희은, 박명애, 정승희, 조영아, 한현자수산생명의학회 · 한국어병학회지2013 · 제26권 제2호질병의 증상을 보이는 해수 양식 어류에서 분리한 비브리오속 세균이화, 김명석, 김수미, 김은전, 박수일, 우승호, 원경미, 조미영, 최광진수산생명의학회 · 한국어병학회지2005 · 제18권 제2호

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