Pentachlorophenol 노출에 따른 넙치, Paralichthys olivaceus의 혈액성분의 변화
Changes of hematological parameters in olive flounder Paralichthys olivaceus exposed to pentachlorophenol
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초록
PCP 노출에 따른 넙치의 혈액성상, 혈청의 유기성분, 무기성분 및 효소활성의 변화를 검토하였다. 넙치의 적혈구 수 및 Ht는 0.5 mg/kg이상의 농도에서2주 이후, Hb는 0.5 mg/kg 이상의 농도에서 4주 이후부터 유의한 감소를 나타냈다. 넙치의 혈청 chloride 및 phosphate 농도의 변화는 관찰되지 않았지만,magnesium 농도는 0.5 mg/kg 이상의 농도에서 4주째이후부터 유의하게 증가하였고, 혈청 calcium농도는0.5 mg/kg 이상의 농도에서 6주째 유의한 감소가 관찰되었다. 혈청 total protein 농도는 6주째 0.5 mg/kg 이상의 농도에서 유의하게 감소하였으며, glucose 농도는 0.5 mg/kg에서 2주 후부터 유의한 증가가 관찰되었지만, total cholesterol 농도의 유의한 차이는 관찰되지 않았다. 혈청 GOT 활성은 0.5 mg/kg의 농도에서2주 이후, 혈청 GPT활성은 0.5 mg/kg 농도에서 4주이후부터 유의한 증가가 관찰되었다. 또한 혈청 LDH 활성은 1.0 mg/kg의 농도에서 4주 이후, 0.5 mg/kg의농도에서 6주에서 유의한 증가가 관찰되었으나, 혈청 AlP활성의 변화는 관찰되지 않았다
Abstract
Experiments were carried out to determine the hematological changes in olive flounder, Paralichthys olivaceus, after sub-chronic dietary pentachlorophenol (PCP) concentrations (0, 0.1, 0.5 and 1.0 mg/kg) exposure 6 weeks. The RBC count, Ht and Hb were significantly decreased in PCP 0.5 mg/kg exposed flounder compared to control group. The flounder exposed to PCP concentration 0.5 mg/kg started to increase significantly in serum magnesium concentrations after 4 weeks. The low concentration of serum calcium concentration was observed at 6 weeks of the experiment in PCP concentration 0.5 mg/kg. Although serum total protein concentrations was significantly reduced compared to the control group at PCP concentration 0.5 mg/kg, serum glucose concentration was found to be significantly increased over the control group. A significant increment of GOT, GPT and LDH activities in the flounder serum was noticed after 4 weeks at PCP concentrations over 0.5 mg/kg without typical changes of Al-P. These results indicate that flounder can be affected by PCP in terms of inorganic matter, organic matter and enzyme activity in serum when they were exposed to the PCP concentrations 0.5 mg/kg or higher.
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